null Mon 24 Dec 07:36:25 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir Job ID is set to 2ooj-1.8-parrot-noncs X-ray data file /users/emra500/scratch/Dataset/noncsChltofom/2ooj-1.8-parrot-noncs.mtz Sequence file /users/emra500/scratch/Dataset/noncsChltofom/2ooj-1.8-parrot-noncs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/2ooj-1.8-parrot-noncs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/2ooj-1.8-parrot-noncs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/2ooj-1.8-parrot-noncs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Mon 24 Dec 07:36:30 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/2ooj-1.8-parrot-noncs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/2ooj-1.8-parrot-noncs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 250 and 0 Target number of residues in the AU: 250 Target solvent content: 0.5111 Checking the provided sequence file Detected sequence length: 141 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 2 Adjusted target number of residues: 282 Adjusted target solvent content: 0.45 Input MTZ file: 2ooj-1.8-parrot-noncs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 19 Cell parameters: 47.211 55.254 106.086 90.000 90.000 90.000 Input sequence file: 2ooj-1.8-parrot-noncs.fasta_lf Building free atoms model in initial map for 2256 target number of atoms Had to go as low as 0.45 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 1 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 53.043 1.840 Wilson plot Bfac: 25.62 24751 reflections ( 99.86 % complete ) and 0 restraints for refining 2516 atoms. Observations/parameters ratio is 2.46 ------------------------------------------------------ Starting model: R = 0.3895 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.3537 (Rfree = 0.000) for 2516 atoms. Found 48 (109 requested) and removed 70 (54 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 2.44 2.61 NCS extension: 0 residues added, 2494 seeds are put forward Round 1: 133 peptides, 27 chains. Longest chain 9 peptides. Score 0.326 Round 2: 147 peptides, 23 chains. Longest chain 13 peptides. Score 0.498 Round 3: 152 peptides, 21 chains. Longest chain 17 peptides. Score 0.564 Round 4: 150 peptides, 19 chains. Longest chain 18 peptides. Score 0.595 Round 5: 152 peptides, 19 chains. Longest chain 18 peptides. Score 0.604 Taking the results from Round 5 Chains 21, Residues 133, Estimated correctness of the model 90.1 % 2 chains (18 residues) have been docked in sequence ------------------------------------------------------ 24751 reflections ( 99.86 % complete ) and 4690 restraints for refining 2219 atoms. 4117 conditional restraints added. Observations/parameters ratio is 2.79 ------------------------------------------------------ Cycle 1: After refmac, R = 0.3812 (Rfree = 0.000) for 2219 atoms. Found 63 (96 requested) and removed 54 (48 requested) atoms. Cycle 2: After refmac, R = 0.3720 (Rfree = 0.000) for 2223 atoms. Found 55 (96 requested) and removed 54 (48 requested) atoms. Cycle 3: After refmac, R = 0.3512 (Rfree = 0.000) for 2218 atoms. Found 38 (96 requested) and removed 49 (48 requested) atoms. Cycle 4: After refmac, R = 0.3476 (Rfree = 0.000) for 2202 atoms. Found 42 (95 requested) and removed 48 (47 requested) atoms. Cycle 5: After refmac, R = 0.3331 (Rfree = 0.000) for 2192 atoms. Found 42 (95 requested) and removed 47 (47 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 2.31 2.47 NCS extension: 10 residues added (2 deleted due to clashes), 2225 seeds are put forward Round 1: 165 peptides, 21 chains. Longest chain 16 peptides. Score 0.621 Round 2: 175 peptides, 17 chains. Longest chain 22 peptides. Score 0.723 Round 3: 166 peptides, 14 chains. Longest chain 22 peptides. Score 0.740 Round 4: 169 peptides, 18 chains. Longest chain 17 peptides. Score 0.688 Round 5: 174 peptides, 17 chains. Longest chain 22 peptides. Score 0.720 Taking the results from Round 3 Chains 15, Residues 152, Estimated correctness of the model 95.4 % 2 chains (21 residues) have been docked in sequence Building loops using Loopy2018 15 chains (152 residues) following loop building 2 chains (21 residues) in sequence following loop building ------------------------------------------------------ 24751 reflections ( 99.86 % complete ) and 4521 restraints for refining 2219 atoms. 3849 conditional restraints added. Observations/parameters ratio is 2.79 ------------------------------------------------------ Cycle 6: After refmac, R = 0.3511 (Rfree = 0.000) for 2219 atoms. Found 60 (96 requested) and removed 49 (48 requested) atoms. Cycle 7: After refmac, R = 0.3324 (Rfree = 0.000) for 2227 atoms. Found 34 (94 requested) and removed 48 (48 requested) atoms. Cycle 8: After refmac, R = 0.3249 (Rfree = 0.000) for 2210 atoms. Found 42 (92 requested) and removed 48 (48 requested) atoms. Cycle 9: After refmac, R = 0.3127 (Rfree = 0.000) for 2201 atoms. Found 46 (89 requested) and removed 30 (47 requested) atoms. Cycle 10: After refmac, R = 0.3048 (Rfree = 0.000) for 2211 atoms. Found 44 (88 requested) and removed 20 (48 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 2.19 2.34 NCS extension: 16 residues added (9 deleted due to clashes), 2257 seeds are put forward Round 1: 172 peptides, 21 chains. Longest chain 14 peptides. Score 0.648 Round 2: 186 peptides, 17 chains. Longest chain 24 peptides. Score 0.756 Round 3: 202 peptides, 15 chains. Longest chain 35 peptides. Score 0.819 Round 4: 206 peptides, 15 chains. Longest chain 29 peptides. Score 0.827 Round 5: 200 peptides, 15 chains. Longest chain 33 peptides. Score 0.814 Taking the results from Round 4 Chains 16, Residues 191, Estimated correctness of the model 97.8 % 4 chains (77 residues) have been docked in sequence Building loops using Loopy2018 16 chains (191 residues) following loop building 4 chains (77 residues) in sequence following loop building ------------------------------------------------------ 24751 reflections ( 99.86 % complete ) and 3546 restraints for refining 2219 atoms. 2498 conditional restraints added. Observations/parameters ratio is 2.79 ------------------------------------------------------ Cycle 11: After refmac, R = 0.3335 (Rfree = 0.000) for 2219 atoms. Found 73 (88 requested) and removed 48 (48 requested) atoms. Cycle 12: After refmac, R = 0.3119 (Rfree = 0.000) for 2240 atoms. Found 40 (87 requested) and removed 24 (48 requested) atoms. Cycle 13: After refmac, R = 0.3047 (Rfree = 0.000) for 2247 atoms. Found 38 (86 requested) and removed 11 (49 requested) atoms. Cycle 14: After refmac, R = 0.2962 (Rfree = 0.000) for 2261 atoms. Found 32 (86 requested) and removed 16 (49 requested) atoms. Cycle 15: After refmac, R = 0.2890 (Rfree = 0.000) for 2271 atoms. Found 31 (86 requested) and removed 11 (49 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 2.08 2.22 NCS extension: 33 residues added (6 deleted due to clashes), 2330 seeds are put forward Round 1: 209 peptides, 14 chains. Longest chain 48 peptides. Score 0.843 Round 2: 211 peptides, 17 chains. Longest chain 29 peptides. Score 0.816 Round 3: 219 peptides, 13 chains. Longest chain 57 peptides. Score 0.868 Round 4: 214 peptides, 17 chains. Longest chain 29 peptides. Score 0.822 Round 5: 224 peptides, 12 chains. Longest chain 107 peptides. Score 0.883 Taking the results from Round 5 Chains 14, Residues 212, Estimated correctness of the model 98.9 % 2 chains (116 residues) have been docked in sequence Building loops using Loopy2018 14 chains (212 residues) following loop building 2 chains (116 residues) in sequence following loop building ------------------------------------------------------ 24751 reflections ( 99.86 % complete ) and 3155 restraints for refining 2244 atoms. 1888 conditional restraints added. Observations/parameters ratio is 2.76 ------------------------------------------------------ Cycle 16: After refmac, R = 0.3055 (Rfree = 0.000) for 2244 atoms. Found 47 (85 requested) and removed 51 (48 requested) atoms. Cycle 17: After refmac, R = 0.2905 (Rfree = 0.000) for 2239 atoms. Found 50 (83 requested) and removed 47 (48 requested) atoms. Cycle 18: After refmac, R = 0.2843 (Rfree = 0.000) for 2240 atoms. Found 38 (81 requested) and removed 24 (48 requested) atoms. Cycle 19: After refmac, R = 0.2754 (Rfree = 0.000) for 2251 atoms. Found 49 (80 requested) and removed 25 (48 requested) atoms. Cycle 20: After refmac, R = 0.2679 (Rfree = 0.000) for 2270 atoms. Found 52 (78 requested) and removed 19 (49 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 2.05 2.19 NCS extension: 25 residues added (33 deleted due to clashes), 2334 seeds are put forward Round 1: 219 peptides, 13 chains. Longest chain 44 peptides. Score 0.868 Round 2: 228 peptides, 12 chains. Longest chain 54 peptides. Score 0.889 Round 3: 228 peptides, 16 chains. Longest chain 48 peptides. Score 0.857 Round 4: 237 peptides, 12 chains. Longest chain 58 peptides. Score 0.899 Round 5: 228 peptides, 13 chains. Longest chain 48 peptides. Score 0.881 Taking the results from Round 4 Chains 13, Residues 225, Estimated correctness of the model 99.2 % 8 chains (190 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 23 A and 30 A Built loop between residues 47 A and 51 A Built loop between residues 59 A and 63 A Built loop between residues 43 B and 46 B Built loop between residues 75 B and 78 B 7 chains (240 residues) following loop building 3 chains (206 residues) in sequence following loop building ------------------------------------------------------ 24751 reflections ( 99.86 % complete ) and 2525 restraints for refining 2320 atoms. 813 conditional restraints added. Observations/parameters ratio is 2.67 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2898 (Rfree = 0.000) for 2320 atoms. Found 71 (80 requested) and removed 53 (50 requested) atoms. Cycle 22: After refmac, R = 0.2733 (Rfree = 0.000) for 2335 atoms. Found 46 (79 requested) and removed 50 (50 requested) atoms. Cycle 23: After refmac, R = 0.2648 (Rfree = 0.000) for 2325 atoms. Found 58 (77 requested) and removed 32 (50 requested) atoms. Cycle 24: After refmac, R = 0.2583 (Rfree = 0.000) for 2348 atoms. Found 49 (75 requested) and removed 31 (51 requested) atoms. Cycle 25: After refmac, R = 0.2520 (Rfree = 0.000) for 2365 atoms. Found 39 (73 requested) and removed 34 (51 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 2.03 2.17 NCS extension: 59 residues added (94 deleted due to clashes), 2433 seeds are put forward Round 1: 245 peptides, 10 chains. Longest chain 43 peptides. Score 0.920 Round 2: 251 peptides, 10 chains. Longest chain 76 peptides. Score 0.925 Round 3: 250 peptides, 6 chains. Longest chain 89 peptides. Score 0.946 Round 4: 252 peptides, 9 chains. Longest chain 56 peptides. Score 0.932 Round 5: 253 peptides, 8 chains. Longest chain 90 peptides. Score 0.937 Taking the results from Round 3 Chains 6, Residues 244, Estimated correctness of the model 99.7 % 6 chains (244 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 40 A and 43 A Built loop between residues 58 A and 62 A Built loop between residues 89 A and 95 A Built loop between residues 39 B and 46 B 2 chains (260 residues) following loop building 2 chains (260 residues) in sequence following loop building ------------------------------------------------------ 24751 reflections ( 99.86 % complete ) and 2246 restraints for refining 2375 atoms. 252 conditional restraints added. Observations/parameters ratio is 2.61 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2611 (Rfree = 0.000) for 2375 atoms. Found 72 (72 requested) and removed 56 (51 requested) atoms. Cycle 27: After refmac, R = 0.2477 (Rfree = 0.000) for 2385 atoms. Found 59 (70 requested) and removed 51 (51 requested) atoms. Cycle 28: After refmac, R = 0.2338 (Rfree = 0.000) for 2391 atoms. Found 60 (68 requested) and removed 51 (51 requested) atoms. Cycle 29: After refmac, R = 0.2267 (Rfree = 0.000) for 2395 atoms. Found 55 (67 requested) and removed 52 (52 requested) atoms. Cycle 30: After refmac, R = 0.2243 (Rfree = 0.000) for 2397 atoms. Found 50 (64 requested) and removed 52 (52 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 2.03 2.17 NCS extension: 38 residues added (94 deleted due to clashes), 2439 seeds are put forward Round 1: 250 peptides, 8 chains. Longest chain 88 peptides. Score 0.935 Round 2: 257 peptides, 3 chains. Longest chain 130 peptides. Score 0.963 Round 3: 253 peptides, 7 chains. Longest chain 54 peptides. Score 0.943 Round 4: 257 peptides, 4 chains. Longest chain 131 peptides. Score 0.959 Round 5: 255 peptides, 7 chains. Longest chain 92 peptides. Score 0.944 Taking the results from Round 2 Chains 3, Residues 254, Estimated correctness of the model 99.9 % 3 chains (254 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 89 B and 95 B 2 chains (259 residues) following loop building 2 chains (259 residues) in sequence following loop building ------------------------------------------------------ 24751 reflections ( 99.86 % complete ) and 2178 restraints for refining 2254 atoms. 196 conditional restraints added. Observations/parameters ratio is 2.75 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2271 (Rfree = 0.000) for 2254 atoms. Found 58 (58 requested) and removed 50 (48 requested) atoms. Cycle 32: After refmac, R = 0.2161 (Rfree = 0.000) for 2262 atoms. Found 57 (57 requested) and removed 49 (49 requested) atoms. Cycle 33: After refmac, R = 0.2108 (Rfree = 0.000) for 2269 atoms. Found 55 (55 requested) and removed 46 (49 requested) atoms. Cycle 34: After refmac, R = 0.2065 (Rfree = 0.000) for 2274 atoms. Found 52 (53 requested) and removed 43 (49 requested) atoms. Cycle 35: After refmac, R = 0.2023 (Rfree = 0.000) for 2280 atoms. Found 51 (51 requested) and removed 42 (49 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 2.02 2.16 NCS extension: 42 residues added (89 deleted due to clashes), 2333 seeds are put forward Round 1: 253 peptides, 5 chains. Longest chain 87 peptides. Score 0.952 Round 2: 256 peptides, 5 chains. Longest chain 94 peptides. Score 0.954 Round 3: 256 peptides, 4 chains. Longest chain 130 peptides. Score 0.959 Round 4: 252 peptides, 6 chains. Longest chain 55 peptides. Score 0.947 Round 5: 256 peptides, 4 chains. Longest chain 130 peptides. Score 0.959 Taking the results from Round 5 Chains 4, Residues 252, Estimated correctness of the model 99.8 % 4 chains (252 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 89 B and 95 B Built loop between residues 113 B and 116 B 2 chains (259 residues) following loop building 2 chains (259 residues) in sequence following loop building ------------------------------------------------------ 24751 reflections ( 99.86 % complete ) and 2198 restraints for refining 2224 atoms. 210 conditional restraints added. Observations/parameters ratio is 2.78 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2131 (Rfree = 0.000) for 2224 atoms. Found 48 (48 requested) and removed 49 (48 requested) atoms. Cycle 37: After refmac, R = 0.2058 (Rfree = 0.000) for 2220 atoms. Found 48 (48 requested) and removed 22 (48 requested) atoms. Cycle 38: After refmac, R = 0.1994 (Rfree = 0.000) for 2245 atoms. Found 48 (48 requested) and removed 18 (48 requested) atoms. Cycle 39: After refmac, R = 0.1983 (Rfree = 0.000) for 2273 atoms. Found 48 (49 requested) and removed 35 (49 requested) atoms. Cycle 40: After refmac, R = 0.1960 (Rfree = 0.000) for 2283 atoms. Found 49 (49 requested) and removed 33 (49 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 2.02 2.16 NCS extension: 0 residues added, 2300 seeds are put forward Round 1: 256 peptides, 5 chains. Longest chain 90 peptides. Score 0.954 Round 2: 257 peptides, 4 chains. Longest chain 131 peptides. Score 0.959 Round 3: 256 peptides, 6 chains. Longest chain 87 peptides. Score 0.949 Round 4: 258 peptides, 5 chains. Longest chain 87 peptides. Score 0.955 Round 5: 256 peptides, 7 chains. Longest chain 91 peptides. Score 0.945 Taking the results from Round 2 Chains 4, Residues 253, Estimated correctness of the model 99.8 % 4 chains (253 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 43 B and 46 B Built loop between residues 89 B and 95 B 2 chains (260 residues) following loop building 2 chains (260 residues) in sequence following loop building ------------------------------------------------------ 24751 reflections ( 99.86 % complete ) and 2187 restraints for refining 2229 atoms. 191 conditional restraints added. Observations/parameters ratio is 2.78 ------------------------------------------------------ Cycle 41: After refmac, R = 0.2119 (Rfree = 0.000) for 2229 atoms. Found 48 (48 requested) and removed 48 (48 requested) atoms. Cycle 42: After refmac, R = 0.2002 (Rfree = 0.000) for 2228 atoms. Found 48 (48 requested) and removed 19 (48 requested) atoms. Cycle 43: After refmac, R = 0.1969 (Rfree = 0.000) for 2254 atoms. Found 49 (49 requested) and removed 29 (49 requested) atoms. Cycle 44: After refmac, R = 0.1929 (Rfree = 0.000) for 2273 atoms. Found 49 (49 requested) and removed 33 (49 requested) atoms. Cycle 45: After refmac, R = 0.1968 (Rfree = 0.000) for 2286 atoms. Found 49 (49 requested) and removed 41 (49 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 2.02 2.16 NCS extension: 38 residues added (94 deleted due to clashes), 2334 seeds are put forward Round 1: 254 peptides, 7 chains. Longest chain 87 peptides. Score 0.943 Round 2: 255 peptides, 5 chains. Longest chain 131 peptides. Score 0.953 Round 3: 255 peptides, 7 chains. Longest chain 87 peptides. Score 0.944 Round 4: 256 peptides, 6 chains. Longest chain 87 peptides. Score 0.949 Round 5: 254 peptides, 7 chains. Longest chain 91 peptides. Score 0.943 Taking the results from Round 2 Last building cycle: Chain fragments will be rearranged Chains 5, Residues 250, Estimated correctness of the model 99.8 % 5 chains (250 residues) have been docked in sequence Sequence coverage is 99 % All DUM atoms will be removed Building loops using Loopy2018 Built loop between residues 43 B and 46 B Built loop between residues 89 B and 95 B Built loop between residues 121 B and 125 B 2 chains (260 residues) following loop building 2 chains (260 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. Update only waters. 24751 reflections ( 99.86 % complete ) and 1996 restraints for refining 1959 atoms. Observations/parameters ratio is 3.16 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2561 (Rfree = 0.000) for 1959 atoms. Found 36 (42 requested) and removed 0 (42 requested) atoms. Cycle 47: After refmac, R = 0.2361 (Rfree = 0.000) for 1959 atoms. Found 19 (43 requested) and removed 1 (43 requested) atoms. Cycle 48: After refmac, R = 0.2236 (Rfree = 0.000) for 1959 atoms. Found 10 (43 requested) and removed 1 (43 requested) atoms. Cycle 49: After refmac, R = 0.2167 (Rfree = 0.000) for 1959 atoms. Found 7 (43 requested) and removed 2 (43 requested) atoms. Writing output files ... Normal termination of warpNtrace Mon 24 Dec 08:11:20 GMT 2018 Job finished. TimeTaking 34.91 Used memory is bytes: 13622376