null Mon 24 Dec 07:57:24 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir Job ID is set to 2avn-3.2-parrot-noncs X-ray data file /users/emra500/scratch/Dataset/noncsChltofom/2avn-3.2-parrot-noncs.mtz Sequence file /users/emra500/scratch/Dataset/noncsChltofom/2avn-3.2-parrot-noncs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/2avn-3.2-parrot-noncs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/2avn-3.2-parrot-noncs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/2avn-3.2-parrot-noncs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Mon 24 Dec 07:57:29 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/2avn-3.2-parrot-noncs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/2avn-3.2-parrot-noncs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 678 and 0 Target number of residues in the AU: 678 Target solvent content: 0.6234 Checking the provided sequence file Detected sequence length: 260 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 2 Adjusted target number of residues: 520 Adjusted target solvent content: 0.71 Input MTZ file: 2avn-3.2-parrot-noncs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 181 Cell parameters: 133.767 133.767 188.622 90.000 90.000 120.000 Input sequence file: 2avn-3.2-parrot-noncs.fasta_lf Building free atoms model in initial map for 4160 target number of atoms Had to go as low as 1.15 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 3 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 115.845 3.200 Wilson plot Bfac: 63.12 17047 reflections ( 99.73 % complete ) and 0 restraints for refining 4662 atoms. Observations/parameters ratio is 0.91 ------------------------------------------------------ Starting model: R = 0.3542 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.3460 (Rfree = 0.000) for 4662 atoms. Found 41 (41 requested) and removed 28 (20 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 3.32 2.46 Search for helices and strands: 0 residues in 0 chains, 4776 seeds are put forward NCS extension: 0 residues added, 4776 seeds are put forward Round 1: 331 peptides, 44 chains. Longest chain 16 peptides. Score 0.464 Round 2: 367 peptides, 40 chains. Longest chain 25 peptides. Score 0.546 Round 3: 397 peptides, 40 chains. Longest chain 27 peptides. Score 0.586 Round 4: 394 peptides, 37 chains. Longest chain 36 peptides. Score 0.601 Round 5: 403 peptides, 35 chains. Longest chain 35 peptides. Score 0.624 Taking the results from Round 5 Chains 42, Residues 368, Estimated correctness of the model 47.4 % 4 chains (63 residues) have been docked in sequence ------------------------------------------------------ 17047 reflections ( 99.73 % complete ) and 7558 restraints for refining 3987 atoms. 5871 conditional restraints added. Observations/parameters ratio is 1.07 ------------------------------------------------------ Cycle 1: After refmac, R = 0.2979 (Rfree = 0.000) for 3987 atoms. Found 35 (35 requested) and removed 25 (17 requested) atoms. Cycle 2: After refmac, R = 0.2908 (Rfree = 0.000) for 3921 atoms. Found 35 (35 requested) and removed 20 (17 requested) atoms. Cycle 3: After refmac, R = 0.2810 (Rfree = 0.000) for 3910 atoms. Found 29 (35 requested) and removed 22 (17 requested) atoms. Cycle 4: After refmac, R = 0.2727 (Rfree = 0.000) for 3907 atoms. Found 30 (35 requested) and removed 23 (17 requested) atoms. Cycle 5: After refmac, R = 0.2611 (Rfree = 0.000) for 3905 atoms. Found 17 (35 requested) and removed 22 (17 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 3.24 2.40 Search for helices and strands: 0 residues in 0 chains, 4055 seeds are put forward NCS extension: 16 residues added (4 deleted due to clashes), 4071 seeds are put forward Round 1: 406 peptides, 34 chains. Longest chain 31 peptides. Score 0.634 Round 2: 410 peptides, 28 chains. Longest chain 39 peptides. Score 0.672 Round 3: 421 peptides, 34 chains. Longest chain 50 peptides. Score 0.651 Round 4: 427 peptides, 32 chains. Longest chain 48 peptides. Score 0.669 Round 5: 438 peptides, 28 chains. Longest chain 39 peptides. Score 0.702 Taking the results from Round 5 Chains 36, Residues 410, Estimated correctness of the model 65.6 % 10 chains (201 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 62 A and 73 A Built loop between residues 110 A and 114 A 30 chains (417 residues) following loop building 8 chains (214 residues) in sequence following loop building ------------------------------------------------------ 17047 reflections ( 99.73 % complete ) and 6489 restraints for refining 4118 atoms. 3948 conditional restraints added. Observations/parameters ratio is 1.03 ------------------------------------------------------ Cycle 6: After refmac, R = 0.2838 (Rfree = 0.000) for 4118 atoms. Found 36 (36 requested) and removed 25 (18 requested) atoms. Cycle 7: After refmac, R = 0.2607 (Rfree = 0.000) for 4085 atoms. Found 36 (36 requested) and removed 23 (18 requested) atoms. Cycle 8: After refmac, R = 0.2488 (Rfree = 0.000) for 4075 atoms. Found 20 (35 requested) and removed 20 (18 requested) atoms. Cycle 9: After refmac, R = 0.2593 (Rfree = 0.000) for 4063 atoms. Found 34 (34 requested) and removed 19 (18 requested) atoms. Cycle 10: After refmac, R = 0.2554 (Rfree = 0.000) for 4064 atoms. Found 33 (33 requested) and removed 23 (18 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 3.19 2.37 Search for helices and strands: 0 residues in 0 chains, 4235 seeds are put forward NCS extension: 96 residues added (61 deleted due to clashes), 4331 seeds are put forward Round 1: 425 peptides, 30 chains. Longest chain 61 peptides. Score 0.678 Round 2: 429 peptides, 31 chains. Longest chain 44 peptides. Score 0.677 Round 3: 411 peptides, 31 chains. Longest chain 42 peptides. Score 0.657 Round 4: 433 peptides, 33 chains. Longest chain 51 peptides. Score 0.670 Round 5: 420 peptides, 28 chains. Longest chain 51 peptides. Score 0.683 Taking the results from Round 5 Chains 31, Residues 392, Estimated correctness of the model 61.5 % 6 chains (157 residues) have been docked in sequence ------------------------------------------------------ 17047 reflections ( 99.73 % complete ) and 7086 restraints for refining 4148 atoms. 4814 conditional restraints added. Observations/parameters ratio is 1.03 ------------------------------------------------------ Cycle 11: After refmac, R = 0.2893 (Rfree = 0.000) for 4148 atoms. Found 33 (33 requested) and removed 33 (18 requested) atoms. Cycle 12: After refmac, R = 0.2619 (Rfree = 0.000) for 4113 atoms. Found 31 (33 requested) and removed 21 (18 requested) atoms. Cycle 13: After refmac, R = 0.2532 (Rfree = 0.000) for 4102 atoms. Found 18 (33 requested) and removed 21 (18 requested) atoms. Cycle 14: After refmac, R = 0.2688 (Rfree = 0.000) for 4080 atoms. Found 32 (32 requested) and removed 23 (18 requested) atoms. Cycle 15: After refmac, R = 0.2373 (Rfree = 0.000) for 4068 atoms. Found 18 (32 requested) and removed 23 (18 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 3.28 2.43 Search for helices and strands: 0 residues in 0 chains, 4176 seeds are put forward NCS extension: 45 residues added (16 deleted due to clashes), 4221 seeds are put forward Round 1: 419 peptides, 36 chains. Longest chain 27 peptides. Score 0.637 Round 2: 432 peptides, 29 chains. Longest chain 54 peptides. Score 0.690 Round 3: 415 peptides, 37 chains. Longest chain 37 peptides. Score 0.627 Round 4: 422 peptides, 33 chains. Longest chain 50 peptides. Score 0.658 Round 5: 421 peptides, 32 chains. Longest chain 31 peptides. Score 0.662 Taking the results from Round 2 Chains 31, Residues 403, Estimated correctness of the model 63.0 % 7 chains (236 residues) have been docked in sequence ------------------------------------------------------ 17047 reflections ( 99.73 % complete ) and 6592 restraints for refining 4245 atoms. 3942 conditional restraints added. Observations/parameters ratio is 1.00 ------------------------------------------------------ Cycle 16: After refmac, R = 0.2756 (Rfree = 0.000) for 4245 atoms. Found 34 (34 requested) and removed 40 (19 requested) atoms. Cycle 17: After refmac, R = 0.2586 (Rfree = 0.000) for 4216 atoms. Found 31 (34 requested) and removed 27 (19 requested) atoms. Cycle 18: After refmac, R = 0.2428 (Rfree = 0.000) for 4203 atoms. Found 25 (33 requested) and removed 22 (18 requested) atoms. Cycle 19: After refmac, R = 0.2346 (Rfree = 0.000) for 4191 atoms. Found 14 (33 requested) and removed 19 (18 requested) atoms. Cycle 20: After refmac, R = 0.2272 (Rfree = 0.000) for 4180 atoms. Found 11 (33 requested) and removed 18 (18 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 3.22 2.39 Search for helices and strands: 0 residues in 0 chains, 4285 seeds are put forward NCS extension: 19 residues added (50 deleted due to clashes), 4304 seeds are put forward Round 1: 410 peptides, 35 chains. Longest chain 34 peptides. Score 0.633 Round 2: 430 peptides, 31 chains. Longest chain 40 peptides. Score 0.678 Round 3: 427 peptides, 34 chains. Longest chain 43 peptides. Score 0.658 Round 4: 418 peptides, 30 chains. Longest chain 35 peptides. Score 0.670 Round 5: 429 peptides, 31 chains. Longest chain 37 peptides. Score 0.677 Taking the results from Round 2 Chains 32, Residues 399, Estimated correctness of the model 60.4 % 9 chains (213 residues) have been docked in sequence ------------------------------------------------------ 17047 reflections ( 99.73 % complete ) and 6755 restraints for refining 4170 atoms. 4275 conditional restraints added. Observations/parameters ratio is 1.02 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2617 (Rfree = 0.000) for 4170 atoms. Found 33 (33 requested) and removed 26 (18 requested) atoms. Cycle 22: After refmac, R = 0.2698 (Rfree = 0.000) for 4157 atoms. Found 33 (33 requested) and removed 21 (18 requested) atoms. Cycle 23: After refmac, R = 0.2731 (Rfree = 0.000) for 4140 atoms. Found 33 (33 requested) and removed 21 (18 requested) atoms. Cycle 24: After refmac, R = 0.2480 (Rfree = 0.000) for 4136 atoms. Found 33 (33 requested) and removed 24 (18 requested) atoms. Cycle 25: After refmac, R = 0.2344 (Rfree = 0.000) for 4128 atoms. Found 33 (33 requested) and removed 22 (18 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 3.23 2.40 Search for helices and strands: 0 residues in 0 chains, 4257 seeds are put forward NCS extension: 42 residues added (18 deleted due to clashes), 4299 seeds are put forward Round 1: 402 peptides, 34 chains. Longest chain 31 peptides. Score 0.629 Round 2: 428 peptides, 33 chains. Longest chain 45 peptides. Score 0.665 Round 3: 420 peptides, 38 chains. Longest chain 34 peptides. Score 0.627 Round 4: 437 peptides, 27 chains. Longest chain 60 peptides. Score 0.706 Round 5: 430 peptides, 39 chains. Longest chain 32 peptides. Score 0.633 Taking the results from Round 4 Chains 30, Residues 410, Estimated correctness of the model 66.4 % 7 chains (213 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 120 B and 124 B 29 chains (412 residues) following loop building 6 chains (216 residues) in sequence following loop building ------------------------------------------------------ 17047 reflections ( 99.73 % complete ) and 6388 restraints for refining 4107 atoms. 3924 conditional restraints added. Observations/parameters ratio is 1.04 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2938 (Rfree = 0.000) for 4107 atoms. Found 32 (32 requested) and removed 44 (18 requested) atoms. Cycle 27: After refmac, R = 0.2658 (Rfree = 0.000) for 4066 atoms. Found 32 (32 requested) and removed 26 (18 requested) atoms. Cycle 28: After refmac, R = 0.2503 (Rfree = 0.000) for 4059 atoms. Found 18 (31 requested) and removed 20 (18 requested) atoms. Cycle 29: After refmac, R = 0.2481 (Rfree = 0.000) for 4038 atoms. Found 20 (30 requested) and removed 24 (18 requested) atoms. Cycle 30: After refmac, R = 0.2436 (Rfree = 0.000) for 4019 atoms. Found 20 (30 requested) and removed 22 (18 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 3.20 2.37 Search for helices and strands: 0 residues in 0 chains, 4138 seeds are put forward NCS extension: 13 residues added (19 deleted due to clashes), 4151 seeds are put forward Round 1: 405 peptides, 32 chains. Longest chain 36 peptides. Score 0.644 Round 2: 421 peptides, 36 chains. Longest chain 43 peptides. Score 0.640 Round 3: 423 peptides, 33 chains. Longest chain 27 peptides. Score 0.659 Round 4: 424 peptides, 36 chains. Longest chain 31 peptides. Score 0.643 Round 5: 406 peptides, 30 chains. Longest chain 32 peptides. Score 0.657 Taking the results from Round 3 Chains 37, Residues 390, Estimated correctness of the model 56.0 % 9 chains (157 residues) have been docked in sequence ------------------------------------------------------ 17047 reflections ( 99.73 % complete ) and 7209 restraints for refining 4104 atoms. 5008 conditional restraints added. Observations/parameters ratio is 1.04 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2681 (Rfree = 0.000) for 4104 atoms. Found 29 (29 requested) and removed 29 (18 requested) atoms. Cycle 32: After refmac, R = 0.2460 (Rfree = 0.000) for 4081 atoms. Found 26 (29 requested) and removed 19 (18 requested) atoms. Cycle 33: After refmac, R = 0.2409 (Rfree = 0.000) for 4070 atoms. Found 22 (29 requested) and removed 20 (18 requested) atoms. Cycle 34: After refmac, R = 0.2337 (Rfree = 0.000) for 4055 atoms. Found 22 (29 requested) and removed 21 (18 requested) atoms. Cycle 35: After refmac, R = 0.2460 (Rfree = 0.000) for 4045 atoms. Found 29 (29 requested) and removed 22 (18 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 3.24 2.40 Search for helices and strands: 0 residues in 0 chains, 4174 seeds are put forward NCS extension: 22 residues added (6 deleted due to clashes), 4196 seeds are put forward Round 1: 410 peptides, 31 chains. Longest chain 54 peptides. Score 0.656 Round 2: 416 peptides, 33 chains. Longest chain 39 peptides. Score 0.651 Round 3: 446 peptides, 29 chains. Longest chain 79 peptides. Score 0.705 Round 4: 415 peptides, 34 chains. Longest chain 35 peptides. Score 0.644 Round 5: 434 peptides, 30 chains. Longest chain 51 peptides. Score 0.687 Taking the results from Round 3 Chains 34, Residues 417, Estimated correctness of the model 66.2 % 10 chains (274 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 135 A and 138 A Built loop between residues 41 B and 47 B Built loop between residues 63 B and 70 B Built loop between residues 197 B and 206 B 27 chains (430 residues) following loop building 6 chains (295 residues) in sequence following loop building ------------------------------------------------------ 17047 reflections ( 99.73 % complete ) and 5758 restraints for refining 4170 atoms. 2879 conditional restraints added. Observations/parameters ratio is 1.02 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2824 (Rfree = 0.000) for 4170 atoms. Found 30 (30 requested) and removed 29 (18 requested) atoms. Cycle 37: After refmac, R = 0.2588 (Rfree = 0.000) for 4136 atoms. Found 29 (29 requested) and removed 20 (18 requested) atoms. Cycle 38: After refmac, R = 0.2533 (Rfree = 0.000) for 4127 atoms. Found 28 (28 requested) and removed 27 (18 requested) atoms. Cycle 39: After refmac, R = 0.2545 (Rfree = 0.000) for 4110 atoms. Found 27 (27 requested) and removed 25 (18 requested) atoms. Cycle 40: After refmac, R = 0.2560 (Rfree = 0.000) for 4079 atoms. Found 26 (26 requested) and removed 22 (18 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 3.31 2.46 Search for helices and strands: 0 residues in 0 chains, 4233 seeds are put forward NCS extension: 78 residues added (108 deleted due to clashes), 4311 seeds are put forward Round 1: 417 peptides, 35 chains. Longest chain 34 peptides. Score 0.641 Round 2: 425 peptides, 28 chains. Longest chain 47 peptides. Score 0.688 Round 3: 415 peptides, 35 chains. Longest chain 33 peptides. Score 0.639 Round 4: 416 peptides, 37 chains. Longest chain 34 peptides. Score 0.628 Round 5: 419 peptides, 34 chains. Longest chain 31 peptides. Score 0.649 Taking the results from Round 2 Chains 32, Residues 397, Estimated correctness of the model 62.6 % 10 chains (238 residues) have been docked in sequence ------------------------------------------------------ 17047 reflections ( 99.73 % complete ) and 6644 restraints for refining 4146 atoms. 4064 conditional restraints added. Observations/parameters ratio is 1.03 ------------------------------------------------------ Cycle 41: After refmac, R = 0.2508 (Rfree = 0.000) for 4146 atoms. Found 26 (26 requested) and removed 28 (18 requested) atoms. Cycle 42: After refmac, R = 0.2632 (Rfree = 0.000) for 4130 atoms. Found 26 (26 requested) and removed 20 (18 requested) atoms. Cycle 43: After refmac, R = 0.2274 (Rfree = 0.000) for 4120 atoms. Found 26 (26 requested) and removed 23 (18 requested) atoms. Cycle 44: After refmac, R = 0.2171 (Rfree = 0.000) for 4113 atoms. Found 25 (25 requested) and removed 20 (18 requested) atoms. Cycle 45: After refmac, R = 0.2141 (Rfree = 0.000) for 4115 atoms. Found 20 (25 requested) and removed 21 (18 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 3.17 2.35 Search for helices and strands: 0 residues in 0 chains, 4210 seeds are put forward NCS extension: 26 residues added (16 deleted due to clashes), 4236 seeds are put forward Round 1: 414 peptides, 37 chains. Longest chain 37 peptides. Score 0.626 Round 2: 434 peptides, 30 chains. Longest chain 38 peptides. Score 0.687 Round 3: 425 peptides, 27 chains. Longest chain 49 peptides. Score 0.694 Round 4: 425 peptides, 31 chains. Longest chain 54 peptides. Score 0.672 Round 5: 429 peptides, 31 chains. Longest chain 32 peptides. Score 0.677 Taking the results from Round 3 Last building cycle: Chain fragments will be rearranged Chains 27, Residues 398, Estimated correctness of the model 63.9 % 9 chains (249 residues) have been docked in sequence Sequence coverage is 62 % Consider running further cycles of model building using 2avn-3_warpNtrace.pdb as input Building loops using Loopy2018 Built loop between residues 104 B and 110 B Built loop between residues 194 B and 198 B 25 chains (406 residues) following loop building 7 chains (257 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. No atom update. 17047 reflections ( 99.73 % complete ) and 6424 restraints for refining 4218 atoms. 3652 conditional restraints added. Observations/parameters ratio is 1.01 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2825 (Rfree = 0.000) for 4218 atoms. Found 0 (26 requested) and removed 18 (18 requested) atoms. Cycle 47: After refmac, R = 0.2797 (Rfree = 0.000) for 4184 atoms. Found 0 (26 requested) and removed 18 (18 requested) atoms. Cycle 48: After refmac, R = 0.2717 (Rfree = 0.000) for 4149 atoms. Found 0 (26 requested) and removed 18 (18 requested) atoms. Cycle 49: After refmac, R = 0.2454 (Rfree = 0.000) for 4110 atoms. Found 0 (26 requested) and removed 18 (18 requested) atoms. Writing output files ... Normal termination of warpNtrace Mon 24 Dec 09:01:30 GMT 2018 Job finished. TimeTaking 64.1 Used memory is bytes: 18501440