null Mon 24 Dec 08:03:19 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir Job ID is set to 1vpm-3.2-parrot-noncs X-ray data file /users/emra500/scratch/Dataset/noncsChltofom/1vpm-3.2-parrot-noncs.mtz Sequence file /users/emra500/scratch/Dataset/noncsChltofom/1vpm-3.2-parrot-noncs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/1vpm-3.2-parrot-noncs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/1vpm-3.2-parrot-noncs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/1vpm-3.2-parrot-noncs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Mon 24 Dec 08:03:24 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/1vpm-3.2-parrot-noncs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/noncs/ArpWArp/wArpResults/WorkingDir/1vpm-3.2-parrot-noncs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 358 and 0 Target number of residues in the AU: 358 Target solvent content: 0.6349 Checking the provided sequence file Detected sequence length: 169 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 3 Adjusted target number of residues: 507 Adjusted target solvent content: 0.48 Input MTZ file: 1vpm-3.2-parrot-noncs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 24 Cell parameters: 82.941 106.484 120.177 90.000 90.000 90.000 Input sequence file: 1vpm-3.2-parrot-noncs.fasta_lf Building free atoms model in initial map for 4056 target number of atoms Had to go as low as 0.55 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 3 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 79.699 3.200 Wilson plot Bfac: 68.98 8944 reflections ( 98.32 % complete ) and 0 restraints for refining 4497 atoms. Observations/parameters ratio is 0.50 ------------------------------------------------------ Starting model: R = 0.3071 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.2937 (Rfree = 0.000) for 4497 atoms. Found 40 (40 requested) and removed 82 (20 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 3.22 3.31 Search for helices and strands: 0 residues in 0 chains, 4522 seeds are put forward NCS extension: 0 residues added, 4522 seeds are put forward Round 1: 259 peptides, 38 chains. Longest chain 17 peptides. Score 0.508 Round 2: 305 peptides, 33 chains. Longest chain 25 peptides. Score 0.664 Round 3: 325 peptides, 30 chains. Longest chain 51 peptides. Score 0.725 Round 4: 329 peptides, 30 chains. Longest chain 28 peptides. Score 0.732 Round 5: 337 peptides, 28 chains. Longest chain 34 peptides. Score 0.759 Taking the results from Round 5 Chains 31, Residues 309, Estimated correctness of the model 76.7 % 7 chains (104 residues) have been docked in sequence Building loops using Loopy2018 31 chains (309 residues) following loop building 7 chains (104 residues) in sequence following loop building ------------------------------------------------------ 8944 reflections ( 98.32 % complete ) and 6985 restraints for refining 3701 atoms. 5408 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 1: After refmac, R = 0.2816 (Rfree = 0.000) for 3701 atoms. Found 28 (33 requested) and removed 64 (16 requested) atoms. Cycle 2: After refmac, R = 0.2388 (Rfree = 0.000) for 3603 atoms. Found 24 (31 requested) and removed 27 (16 requested) atoms. Cycle 3: After refmac, R = 0.2442 (Rfree = 0.000) for 3576 atoms. Found 25 (31 requested) and removed 28 (16 requested) atoms. Cycle 4: After refmac, R = 0.2237 (Rfree = 0.000) for 3554 atoms. Found 16 (30 requested) and removed 20 (16 requested) atoms. Cycle 5: After refmac, R = 0.2151 (Rfree = 0.000) for 3533 atoms. Found 13 (29 requested) and removed 26 (15 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 3.22 3.31 Search for helices and strands: 0 residues in 0 chains, 3620 seeds are put forward NCS extension: 24 residues added (14 deleted due to clashes), 3644 seeds are put forward Round 1: 318 peptides, 33 chains. Longest chain 43 peptides. Score 0.689 Round 2: 338 peptides, 30 chains. Longest chain 44 peptides. Score 0.746 Round 3: 357 peptides, 31 chains. Longest chain 58 peptides. Score 0.767 Round 4: 344 peptides, 27 chains. Longest chain 50 peptides. Score 0.776 Round 5: 343 peptides, 32 chains. Longest chain 31 peptides. Score 0.739 Taking the results from Round 4 Chains 37, Residues 317, Estimated correctness of the model 79.6 % 7 chains (127 residues) have been docked in sequence Building loops using Loopy2018 37 chains (317 residues) following loop building 7 chains (127 residues) in sequence following loop building ------------------------------------------------------ 8944 reflections ( 98.32 % complete ) and 6840 restraints for refining 3702 atoms. 5122 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 6: After refmac, R = 0.2578 (Rfree = 0.000) for 3702 atoms. Found 18 (30 requested) and removed 35 (16 requested) atoms. Cycle 7: After refmac, R = 0.2654 (Rfree = 0.000) for 3664 atoms. Found 18 (29 requested) and removed 29 (16 requested) atoms. Cycle 8: After refmac, R = 0.2275 (Rfree = 0.000) for 3629 atoms. Found 12 (28 requested) and removed 21 (16 requested) atoms. Cycle 9: After refmac, R = 0.2315 (Rfree = 0.000) for 3605 atoms. Found 15 (27 requested) and removed 19 (16 requested) atoms. Cycle 10: After refmac, R = 0.2173 (Rfree = 0.000) for 3593 atoms. Found 13 (26 requested) and removed 22 (16 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 3.22 3.31 Search for helices and strands: 0 residues in 0 chains, 3686 seeds are put forward NCS extension: 37 residues added (43 deleted due to clashes), 3723 seeds are put forward Round 1: 326 peptides, 32 chains. Longest chain 38 peptides. Score 0.711 Round 2: 343 peptides, 29 chains. Longest chain 33 peptides. Score 0.761 Round 3: 350 peptides, 28 chains. Longest chain 66 peptides. Score 0.777 Round 4: 339 peptides, 34 chains. Longest chain 33 peptides. Score 0.717 Round 5: 327 peptides, 26 chains. Longest chain 62 peptides. Score 0.759 Taking the results from Round 3 Chains 28, Residues 322, Estimated correctness of the model 79.8 % 3 chains (87 residues) have been docked in sequence Building loops using Loopy2018 28 chains (322 residues) following loop building 3 chains (87 residues) in sequence following loop building ------------------------------------------------------ 8944 reflections ( 98.32 % complete ) and 7306 restraints for refining 3683 atoms. 5738 conditional restraints added. Observations/parameters ratio is 0.61 ------------------------------------------------------ Cycle 11: After refmac, R = 0.2371 (Rfree = 0.000) for 3683 atoms. Found 20 (26 requested) and removed 40 (16 requested) atoms. Cycle 12: After refmac, R = 0.2203 (Rfree = 0.000) for 3647 atoms. Found 10 (25 requested) and removed 24 (16 requested) atoms. Cycle 13: After refmac, R = 0.2268 (Rfree = 0.000) for 3626 atoms. Found 13 (24 requested) and removed 27 (16 requested) atoms. Cycle 14: After refmac, R = 0.2293 (Rfree = 0.000) for 3601 atoms. Found 7 (24 requested) and removed 24 (16 requested) atoms. Cycle 15: After refmac, R = 0.2226 (Rfree = 0.000) for 3576 atoms. Found 10 (23 requested) and removed 22 (16 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 3.16 3.25 Search for helices and strands: 0 residues in 0 chains, 3673 seeds are put forward NCS extension: 46 residues added (54 deleted due to clashes), 3719 seeds are put forward Round 1: 325 peptides, 38 chains. Longest chain 36 peptides. Score 0.658 Round 2: 345 peptides, 35 chains. Longest chain 42 peptides. Score 0.719 Round 3: 345 peptides, 33 chains. Longest chain 44 peptides. Score 0.735 Round 4: 356 peptides, 31 chains. Longest chain 48 peptides. Score 0.765 Round 5: 362 peptides, 34 chains. Longest chain 60 peptides. Score 0.753 Taking the results from Round 4 Chains 33, Residues 325, Estimated correctness of the model 77.7 % 4 chains (106 residues) have been docked in sequence Building loops using Loopy2018 33 chains (325 residues) following loop building 4 chains (106 residues) in sequence following loop building ------------------------------------------------------ 8944 reflections ( 98.32 % complete ) and 6889 restraints for refining 3682 atoms. 5221 conditional restraints added. Observations/parameters ratio is 0.61 ------------------------------------------------------ Cycle 16: After refmac, R = 0.2728 (Rfree = 0.000) for 3682 atoms. Found 23 (23 requested) and removed 42 (16 requested) atoms. Cycle 17: After refmac, R = 0.2642 (Rfree = 0.000) for 3647 atoms. Found 22 (22 requested) and removed 28 (16 requested) atoms. Cycle 18: After refmac, R = 0.2174 (Rfree = 0.000) for 3632 atoms. Found 19 (21 requested) and removed 28 (16 requested) atoms. Cycle 19: After refmac, R = 0.2137 (Rfree = 0.000) for 3616 atoms. Found 16 (20 requested) and removed 23 (16 requested) atoms. Cycle 20: After refmac, R = 0.1990 (Rfree = 0.000) for 3607 atoms. Found 10 (20 requested) and removed 22 (16 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 3.19 3.28 Search for helices and strands: 0 residues in 0 chains, 3717 seeds are put forward NCS extension: 26 residues added (32 deleted due to clashes), 3743 seeds are put forward Round 1: 305 peptides, 38 chains. Longest chain 24 peptides. Score 0.617 Round 2: 338 peptides, 33 chains. Longest chain 51 peptides. Score 0.723 Round 3: 337 peptides, 31 chains. Longest chain 51 peptides. Score 0.737 Round 4: 323 peptides, 34 chains. Longest chain 41 peptides. Score 0.689 Round 5: 321 peptides, 33 chains. Longest chain 44 peptides. Score 0.694 Taking the results from Round 3 Chains 33, Residues 306, Estimated correctness of the model 72.6 % 3 chains (64 residues) have been docked in sequence Building loops using Loopy2018 33 chains (306 residues) following loop building 3 chains (64 residues) in sequence following loop building ------------------------------------------------------ 8944 reflections ( 98.32 % complete ) and 7402 restraints for refining 3703 atoms. 5964 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2392 (Rfree = 0.000) for 3703 atoms. Found 20 (20 requested) and removed 29 (16 requested) atoms. Cycle 22: After refmac, R = 0.2421 (Rfree = 0.000) for 3686 atoms. Found 16 (19 requested) and removed 21 (16 requested) atoms. Cycle 23: After refmac, R = 0.2478 (Rfree = 0.000) for 3667 atoms. Found 18 (18 requested) and removed 25 (16 requested) atoms. Cycle 24: After refmac, R = 0.2269 (Rfree = 0.000) for 3650 atoms. Found 17 (17 requested) and removed 21 (16 requested) atoms. Cycle 25: After refmac, R = 0.2281 (Rfree = 0.000) for 3636 atoms. Found 12 (17 requested) and removed 23 (16 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 3.21 3.30 Search for helices and strands: 0 residues in 0 chains, 3699 seeds are put forward NCS extension: 10 residues added (11 deleted due to clashes), 3709 seeds are put forward Round 1: 300 peptides, 41 chains. Longest chain 23 peptides. Score 0.576 Round 2: 341 peptides, 36 chains. Longest chain 38 peptides. Score 0.705 Round 3: 310 peptides, 35 chains. Longest chain 45 peptides. Score 0.656 Round 4: 321 peptides, 36 chains. Longest chain 23 peptides. Score 0.668 Round 5: 325 peptides, 33 chains. Longest chain 34 peptides. Score 0.701 Taking the results from Round 2 Chains 42, Residues 305, Estimated correctness of the model 66.2 % 5 chains (84 residues) have been docked in sequence Building loops using Loopy2018 42 chains (305 residues) following loop building 5 chains (84 residues) in sequence following loop building ------------------------------------------------------ 8944 reflections ( 98.32 % complete ) and 7178 restraints for refining 3690 atoms. 5697 conditional restraints added. Observations/parameters ratio is 0.61 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2666 (Rfree = 0.000) for 3690 atoms. Found 16 (16 requested) and removed 32 (16 requested) atoms. Cycle 27: After refmac, R = 0.2440 (Rfree = 0.000) for 3661 atoms. Found 16 (16 requested) and removed 25 (16 requested) atoms. Cycle 28: After refmac, R = 0.2263 (Rfree = 0.000) for 3649 atoms. Found 16 (16 requested) and removed 26 (16 requested) atoms. Cycle 29: After refmac, R = 0.2111 (Rfree = 0.000) for 3632 atoms. Found 8 (16 requested) and removed 20 (16 requested) atoms. Cycle 30: After refmac, R = 0.2115 (Rfree = 0.000) for 3618 atoms. Found 5 (16 requested) and removed 18 (16 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 3.19 3.28 Search for helices and strands: 0 residues in 0 chains, 3680 seeds are put forward NCS extension: 11 residues added (7 deleted due to clashes), 3691 seeds are put forward Round 1: 298 peptides, 38 chains. Longest chain 34 peptides. Score 0.602 Round 2: 304 peptides, 32 chains. Longest chain 36 peptides. Score 0.671 Round 3: 299 peptides, 34 chains. Longest chain 28 peptides. Score 0.643 Round 4: 295 peptides, 32 chains. Longest chain 35 peptides. Score 0.654 Round 5: 296 peptides, 34 chains. Longest chain 22 peptides. Score 0.637 Taking the results from Round 2 Chains 36, Residues 272, Estimated correctness of the model 58.8 % 4 chains (72 residues) have been docked in sequence ------------------------------------------------------ 8944 reflections ( 98.32 % complete ) and 7295 restraints for refining 3688 atoms. 5983 conditional restraints added. Observations/parameters ratio is 0.61 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2462 (Rfree = 0.000) for 3688 atoms. Found 16 (16 requested) and removed 31 (16 requested) atoms. Cycle 32: After refmac, R = 0.2306 (Rfree = 0.000) for 3666 atoms. Found 16 (16 requested) and removed 21 (16 requested) atoms. Cycle 33: After refmac, R = 0.2674 (Rfree = 0.000) for 3656 atoms. Found 16 (16 requested) and removed 32 (16 requested) atoms. Cycle 34: After refmac, R = 0.2307 (Rfree = 0.000) for 3636 atoms. Found 16 (16 requested) and removed 20 (16 requested) atoms. Cycle 35: After refmac, R = 0.2577 (Rfree = 0.000) for 3629 atoms. Found 16 (16 requested) and removed 19 (16 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 3.22 3.31 Search for helices and strands: 0 residues in 0 chains, 3723 seeds are put forward NCS extension: 16 residues added (6 deleted due to clashes), 3739 seeds are put forward Round 1: 274 peptides, 33 chains. Longest chain 32 peptides. Score 0.599 Round 2: 300 peptides, 32 chains. Longest chain 33 peptides. Score 0.664 Round 3: 296 peptides, 30 chains. Longest chain 33 peptides. Score 0.674 Round 4: 299 peptides, 30 chains. Longest chain 25 peptides. Score 0.680 Round 5: 313 peptides, 31 chains. Longest chain 33 peptides. Score 0.697 Taking the results from Round 5 Chains 34, Residues 282, Estimated correctness of the model 64.5 % 3 chains (41 residues) have been docked in sequence ------------------------------------------------------ 8944 reflections ( 98.32 % complete ) and 7556 restraints for refining 3703 atoms. 6287 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2427 (Rfree = 0.000) for 3703 atoms. Found 16 (16 requested) and removed 28 (16 requested) atoms. Cycle 37: After refmac, R = 0.2345 (Rfree = 0.000) for 3688 atoms. Found 11 (16 requested) and removed 20 (16 requested) atoms. Cycle 38: After refmac, R = 0.2772 (Rfree = 0.000) for 3675 atoms. Found 16 (16 requested) and removed 25 (16 requested) atoms. Cycle 39: After refmac, R = 0.2485 (Rfree = 0.000) for 3658 atoms. Found 16 (16 requested) and removed 20 (16 requested) atoms. Cycle 40: After refmac, R = 0.2596 (Rfree = 0.000) for 3646 atoms. Found 16 (16 requested) and removed 23 (16 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 3.20 3.29 Search for helices and strands: 0 residues in 0 chains, 3726 seeds are put forward NCS extension: 21 residues added (6 deleted due to clashes), 3747 seeds are put forward Round 1: 250 peptides, 34 chains. Longest chain 18 peptides. Score 0.530 Round 2: 288 peptides, 32 chains. Longest chain 23 peptides. Score 0.639 Round 3: 291 peptides, 31 chains. Longest chain 20 peptides. Score 0.655 Round 4: 274 peptides, 28 chains. Longest chain 32 peptides. Score 0.649 Round 5: 276 peptides, 30 chains. Longest chain 27 peptides. Score 0.634 Taking the results from Round 3 Chains 33, Residues 260, Estimated correctness of the model 55.0 % 4 chains (38 residues) have been docked in sequence ------------------------------------------------------ 8944 reflections ( 98.32 % complete ) and 7658 restraints for refining 3702 atoms. 6504 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 41: After refmac, R = 0.2401 (Rfree = 0.000) for 3702 atoms. Found 16 (16 requested) and removed 41 (16 requested) atoms. Cycle 42: After refmac, R = 0.2226 (Rfree = 0.000) for 3671 atoms. Found 15 (16 requested) and removed 20 (16 requested) atoms. Cycle 43: After refmac, R = 0.2081 (Rfree = 0.000) for 3660 atoms. Found 5 (16 requested) and removed 20 (16 requested) atoms. Cycle 44: After refmac, R = 0.2071 (Rfree = 0.000) for 3643 atoms. Found 7 (16 requested) and removed 18 (16 requested) atoms. Cycle 45: After refmac, R = 0.2072 (Rfree = 0.000) for 3630 atoms. Found 12 (16 requested) and removed 21 (16 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 3.21 3.30 Search for helices and strands: 0 residues in 0 chains, 3715 seeds are put forward NCS extension: 15 residues added (3 deleted due to clashes), 3730 seeds are put forward Round 1: 250 peptides, 31 chains. Longest chain 16 peptides. Score 0.564 Round 2: 273 peptides, 31 chains. Longest chain 17 peptides. Score 0.617 Round 3: 271 peptides, 30 chains. Longest chain 25 peptides. Score 0.623 Round 4: 266 peptides, 29 chains. Longest chain 32 peptides. Score 0.622 Round 5: 274 peptides, 28 chains. Longest chain 32 peptides. Score 0.649 Taking the results from Round 5 Last building cycle: Chain fragments will be rearranged Chains 28, Residues 246, Estimated correctness of the model 53.6 % 2 chains (48 residues) have been docked in sequence Sequence coverage is 19 % Consider running further cycles of model building using 1vpm-3_warpNtrace.pdb as input Building loops using Loopy2018 28 chains (246 residues) following loop building 2 chains (48 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. No atom update. 8944 reflections ( 98.32 % complete ) and 7677 restraints for refining 3702 atoms. 6524 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2399 (Rfree = 0.000) for 3702 atoms. Found 0 (16 requested) and removed 16 (16 requested) atoms. Cycle 47: After refmac, R = 0.2277 (Rfree = 0.000) for 3677 atoms. Found 0 (16 requested) and removed 16 (16 requested) atoms. Cycle 48: After refmac, R = 0.2445 (Rfree = 0.000) for 3659 atoms. Found 0 (16 requested) and removed 16 (16 requested) atoms. Cycle 49: After refmac, R = 0.2304 (Rfree = 0.000) for 3640 atoms. Found 0 (16 requested) and removed 16 (16 requested) atoms. Writing output files ... Normal termination of warpNtrace Mon 24 Dec 08:54:11 GMT 2018 Job finished. TimeTaking 50.86 Used memory is bytes: 13961368