Mon 24 Dec 00:37:28 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir Job ID is set to 2f4p-1.9-parrot-mrncs X-ray data file /users/emra500/scratch/Dataset/mrncsChltofom/2f4p-1.9-parrot-mrncs.mtz Sequence file /users/emra500/scratch/Dataset/mrncsChltofom/2f4p-1.9-parrot-mrncs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir/2f4p-1.9-parrot-mrncs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir/2f4p-1.9-parrot-mrncs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir/2f4p-1.9-parrot-mrncs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Mon 24 Dec 00:37:33 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir/2f4p-1.9-parrot-mrncs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir/2f4p-1.9-parrot-mrncs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 529 and 0 Target number of residues in the AU: 529 Target solvent content: 0.4892 Checking the provided sequence file Detected sequence length: 147 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 4 Adjusted target number of residues: 588 Adjusted target solvent content: 0.43 Input MTZ file: 2f4p-1.9-parrot-mrncs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 170 Cell parameters: 71.746 71.746 188.600 90.000 90.000 120.000 Input sequence file: 2f4p-1.9-parrot-mrncs.fasta_lf Building free atoms model in initial map for 4704 target number of atoms Had to go as low as 0.45 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 1 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 21.543 1.900 Wilson plot Bfac: 21.23 43044 reflections ( 99.90 % complete ) and 0 restraints for refining 5217 atoms. Observations/parameters ratio is 2.06 ------------------------------------------------------ Starting model: R = 0.3371 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.2837 (Rfree = 0.000) for 5217 atoms. Found 193 (206 requested) and removed 108 (103 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 1.95 2.14 NCS extension: 0 residues added, 5302 seeds are put forward Round 1: 418 peptides, 37 chains. Longest chain 40 peptides. Score 0.792 Round 2: 473 peptides, 22 chains. Longest chain 93 peptides. Score 0.903 Round 3: 482 peptides, 18 chains. Longest chain 99 peptides. Score 0.920 Round 4: 499 peptides, 13 chains. Longest chain 100 peptides. Score 0.941 Round 5: 508 peptides, 10 chains. Longest chain 101 peptides. Score 0.951 Taking the results from Round 5 Chains 12, Residues 498, Estimated correctness of the model 99.8 % 8 chains (474 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 42 A and 46 A Built loop between residues 65 B and 68 B Built loop between residues 108 D and 111 D 9 chains (505 residues) following loop building 5 chains (481 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4766 restraints for refining 4625 atoms. 758 conditional restraints added. Observations/parameters ratio is 2.33 ------------------------------------------------------ Cycle 1: After refmac, R = 0.3044 (Rfree = 0.000) for 4625 atoms. Found 154 (183 requested) and removed 95 (91 requested) atoms. Cycle 2: After refmac, R = 0.2648 (Rfree = 0.000) for 4667 atoms. Found 145 (181 requested) and removed 68 (92 requested) atoms. Cycle 3: After refmac, R = 0.2472 (Rfree = 0.000) for 4726 atoms. Found 81 (184 requested) and removed 38 (94 requested) atoms. Cycle 4: After refmac, R = 0.2351 (Rfree = 0.000) for 4750 atoms. Found 95 (185 requested) and removed 22 (94 requested) atoms. Cycle 5: After refmac, R = 0.2263 (Rfree = 0.000) for 4808 atoms. Found 93 (187 requested) and removed 21 (95 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 1.92 2.11 NCS extension: 7 residues added (37 deleted due to clashes), 4890 seeds are put forward Round 1: 507 peptides, 7 chains. Longest chain 125 peptides. Score 0.958 Round 2: 505 peptides, 9 chains. Longest chain 100 peptides. Score 0.953 Round 3: 509 peptides, 8 chains. Longest chain 129 peptides. Score 0.956 Round 4: 508 peptides, 8 chains. Longest chain 129 peptides. Score 0.956 Round 5: 510 peptides, 6 chains. Longest chain 128 peptides. Score 0.961 Taking the results from Round 5 Chains 9, Residues 504, Estimated correctness of the model 99.8 % 6 chains (500 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 40 C and 46 C Built loop between residues 41 B and 46 B 6 chains (511 residues) following loop building 4 chains (509 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4641 restraints for refining 4736 atoms. 488 conditional restraints added. Observations/parameters ratio is 2.27 ------------------------------------------------------ Cycle 6: After refmac, R = 0.2318 (Rfree = 0.000) for 4736 atoms. Found 147 (183 requested) and removed 95 (93 requested) atoms. Cycle 7: After refmac, R = 0.2200 (Rfree = 0.000) for 4784 atoms. Found 116 (181 requested) and removed 34 (94 requested) atoms. Cycle 8: After refmac, R = 0.2124 (Rfree = 0.000) for 4857 atoms. Found 100 (184 requested) and removed 21 (96 requested) atoms. Cycle 9: After refmac, R = 0.2055 (Rfree = 0.000) for 4928 atoms. Found 96 (187 requested) and removed 33 (97 requested) atoms. Cycle 10: After refmac, R = 0.2021 (Rfree = 0.000) for 4984 atoms. Found 93 (189 requested) and removed 35 (98 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 1.91 2.10 NCS extension: 8 residues added (52 deleted due to clashes), 5059 seeds are put forward Round 1: 510 peptides, 7 chains. Longest chain 128 peptides. Score 0.959 Round 2: 511 peptides, 8 chains. Longest chain 123 peptides. Score 0.957 Round 3: 512 peptides, 7 chains. Longest chain 126 peptides. Score 0.959 Round 4: 512 peptides, 7 chains. Longest chain 132 peptides. Score 0.959 Round 5: 510 peptides, 9 chains. Longest chain 100 peptides. Score 0.954 Taking the results from Round 4 Chains 7, Residues 505, Estimated correctness of the model 99.8 % 7 chains (505 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 45 A and 48 A Built loop between residues 107 A and 110 A Built loop between residues 42 C and 48 C 4 chains (514 residues) following loop building 4 chains (514 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4722 restraints for refining 4869 atoms. 534 conditional restraints added. Observations/parameters ratio is 2.21 ------------------------------------------------------ Cycle 11: After refmac, R = 0.2173 (Rfree = 0.000) for 4869 atoms. Found 152 (185 requested) and removed 100 (96 requested) atoms. Cycle 12: After refmac, R = 0.2074 (Rfree = 0.000) for 4913 atoms. Found 94 (183 requested) and removed 28 (97 requested) atoms. Cycle 13: After refmac, R = 0.2007 (Rfree = 0.000) for 4972 atoms. Found 88 (185 requested) and removed 25 (98 requested) atoms. Cycle 14: After refmac, R = 0.1973 (Rfree = 0.000) for 5029 atoms. Found 83 (187 requested) and removed 34 (99 requested) atoms. Cycle 15: After refmac, R = 0.1944 (Rfree = 0.000) for 5070 atoms. Found 104 (189 requested) and removed 27 (100 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 1.91 2.10 NCS extension: 0 residues added, 5149 seeds are put forward Round 1: 512 peptides, 7 chains. Longest chain 128 peptides. Score 0.959 Round 2: 513 peptides, 8 chains. Longest chain 101 peptides. Score 0.957 Round 3: 516 peptides, 8 chains. Longest chain 128 peptides. Score 0.958 Round 4: 518 peptides, 6 chains. Longest chain 132 peptides. Score 0.963 Round 5: 517 peptides, 6 chains. Longest chain 131 peptides. Score 0.963 Taking the results from Round 5 Chains 7, Residues 511, Estimated correctness of the model 99.9 % 6 chains (509 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 65 A and 68 A Built loop between residues 42 C and 46 C 5 chains (516 residues) following loop building 4 chains (514 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4722 restraints for refining 4911 atoms. 527 conditional restraints added. Observations/parameters ratio is 2.19 ------------------------------------------------------ Cycle 16: After refmac, R = 0.2102 (Rfree = 0.000) for 4911 atoms. Found 165 (182 requested) and removed 100 (97 requested) atoms. Cycle 17: After refmac, R = 0.2033 (Rfree = 0.000) for 4976 atoms. Found 102 (181 requested) and removed 50 (98 requested) atoms. Cycle 18: After refmac, R = 0.1990 (Rfree = 0.000) for 5019 atoms. Found 100 (183 requested) and removed 39 (99 requested) atoms. Cycle 19: After refmac, R = 0.1955 (Rfree = 0.000) for 5073 atoms. Found 84 (185 requested) and removed 29 (100 requested) atoms. Cycle 20: After refmac, R = 0.1936 (Rfree = 0.000) for 5119 atoms. Found 84 (187 requested) and removed 41 (101 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 1.91 2.10 NCS extension: 1 residues added (23 deleted due to clashes), 5165 seeds are put forward Round 1: 513 peptides, 5 chains. Longest chain 131 peptides. Score 0.964 Round 2: 511 peptides, 7 chains. Longest chain 131 peptides. Score 0.959 Round 3: 512 peptides, 6 chains. Longest chain 131 peptides. Score 0.961 Round 4: 510 peptides, 8 chains. Longest chain 131 peptides. Score 0.956 Round 5: 511 peptides, 7 chains. Longest chain 127 peptides. Score 0.959 Taking the results from Round 1 Chains 6, Residues 508, Estimated correctness of the model 99.9 % 5 chains (507 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 41 D and 46 D 4 chains (511 residues) following loop building 4 chains (511 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4822 restraints for refining 4977 atoms. 657 conditional restraints added. Observations/parameters ratio is 2.16 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2085 (Rfree = 0.000) for 4977 atoms. Found 166 (181 requested) and removed 84 (98 requested) atoms. Cycle 22: After refmac, R = 0.2016 (Rfree = 0.000) for 5050 atoms. Found 107 (180 requested) and removed 42 (100 requested) atoms. Cycle 23: After refmac, R = 0.1965 (Rfree = 0.000) for 5113 atoms. Found 76 (182 requested) and removed 43 (101 requested) atoms. Cycle 24: After refmac, R = 0.1922 (Rfree = 0.000) for 5139 atoms. Found 87 (180 requested) and removed 37 (102 requested) atoms. Cycle 25: After refmac, R = 0.1905 (Rfree = 0.000) for 5180 atoms. Found 72 (180 requested) and removed 42 (102 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 1.91 2.10 NCS extension: 0 residues added, 5214 seeds are put forward Round 1: 511 peptides, 5 chains. Longest chain 131 peptides. Score 0.963 Round 2: 509 peptides, 6 chains. Longest chain 131 peptides. Score 0.961 Round 3: 512 peptides, 7 chains. Longest chain 131 peptides. Score 0.959 Round 4: 509 peptides, 8 chains. Longest chain 127 peptides. Score 0.956 Round 5: 508 peptides, 8 chains. Longest chain 131 peptides. Score 0.956 Taking the results from Round 1 Chains 5, Residues 506, Estimated correctness of the model 99.9 % 5 chains (506 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 41 D and 46 D 4 chains (510 residues) following loop building 4 chains (510 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4861 restraints for refining 5001 atoms. 707 conditional restraints added. Observations/parameters ratio is 2.15 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2075 (Rfree = 0.000) for 5001 atoms. Found 167 (170 requested) and removed 94 (99 requested) atoms. Cycle 27: After refmac, R = 0.2002 (Rfree = 0.000) for 5066 atoms. Found 102 (169 requested) and removed 43 (100 requested) atoms. Cycle 28: After refmac, R = 0.1936 (Rfree = 0.000) for 5121 atoms. Found 84 (171 requested) and removed 21 (101 requested) atoms. Cycle 29: After refmac, R = 0.1903 (Rfree = 0.000) for 5176 atoms. Found 69 (172 requested) and removed 35 (102 requested) atoms. Cycle 30: After refmac, R = 0.1872 (Rfree = 0.000) for 5203 atoms. Found 77 (169 requested) and removed 23 (103 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 1.91 2.10 NCS extension: 3 residues added (0 deleted due to clashes), 5263 seeds are put forward Round 1: 510 peptides, 5 chains. Longest chain 130 peptides. Score 0.963 Round 2: 509 peptides, 7 chains. Longest chain 130 peptides. Score 0.958 Round 3: 507 peptides, 8 chains. Longest chain 102 peptides. Score 0.956 Round 4: 509 peptides, 8 chains. Longest chain 126 peptides. Score 0.956 Round 5: 506 peptides, 11 chains. Longest chain 92 peptides. Score 0.948 Taking the results from Round 1 Chains 6, Residues 505, Estimated correctness of the model 99.9 % 5 chains (504 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 41 D and 46 D 5 chains (509 residues) following loop building 5 chains (509 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4855 restraints for refining 5019 atoms. 711 conditional restraints added. Observations/parameters ratio is 2.14 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2051 (Rfree = 0.000) for 5019 atoms. Found 163 (163 requested) and removed 73 (99 requested) atoms. Cycle 32: After refmac, R = 0.1989 (Rfree = 0.000) for 5098 atoms. Found 89 (166 requested) and removed 30 (101 requested) atoms. Cycle 33: After refmac, R = 0.1926 (Rfree = 0.000) for 5148 atoms. Found 79 (167 requested) and removed 23 (102 requested) atoms. Cycle 34: After refmac, R = 0.1902 (Rfree = 0.000) for 5199 atoms. Found 62 (169 requested) and removed 37 (103 requested) atoms. Cycle 35: After refmac, R = 0.1871 (Rfree = 0.000) for 5211 atoms. Found 77 (166 requested) and removed 38 (103 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 1.91 2.10 NCS extension: 0 residues added, 5252 seeds are put forward Round 1: 507 peptides, 6 chains. Longest chain 130 peptides. Score 0.960 Round 2: 504 peptides, 9 chains. Longest chain 125 peptides. Score 0.952 Round 3: 508 peptides, 7 chains. Longest chain 127 peptides. Score 0.958 Round 4: 506 peptides, 7 chains. Longest chain 130 peptides. Score 0.958 Round 5: 509 peptides, 7 chains. Longest chain 130 peptides. Score 0.958 Taking the results from Round 1 Chains 6, Residues 501, Estimated correctness of the model 99.8 % 6 chains (501 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 41 A and 48 A Built loop between residues 122 D and 125 D 4 chains (509 residues) following loop building 4 chains (509 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4882 restraints for refining 5031 atoms. 733 conditional restraints added. Observations/parameters ratio is 2.14 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2049 (Rfree = 0.000) for 5031 atoms. Found 159 (159 requested) and removed 85 (99 requested) atoms. Cycle 37: After refmac, R = 0.1982 (Rfree = 0.000) for 5095 atoms. Found 110 (158 requested) and removed 34 (101 requested) atoms. Cycle 38: After refmac, R = 0.1918 (Rfree = 0.000) for 5168 atoms. Found 82 (160 requested) and removed 42 (102 requested) atoms. Cycle 39: After refmac, R = 0.1891 (Rfree = 0.000) for 5205 atoms. Found 71 (157 requested) and removed 30 (103 requested) atoms. Cycle 40: After refmac, R = 0.1864 (Rfree = 0.000) for 5237 atoms. Found 65 (158 requested) and removed 43 (104 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 1.91 2.10 NCS extension: 0 residues added, 5261 seeds are put forward Round 1: 509 peptides, 6 chains. Longest chain 130 peptides. Score 0.961 Round 2: 508 peptides, 8 chains. Longest chain 126 peptides. Score 0.956 Round 3: 512 peptides, 7 chains. Longest chain 130 peptides. Score 0.959 Round 4: 506 peptides, 8 chains. Longest chain 125 peptides. Score 0.955 Round 5: 508 peptides, 11 chains. Longest chain 91 peptides. Score 0.949 Taking the results from Round 1 Chains 7, Residues 503, Estimated correctness of the model 99.8 % 6 chains (502 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 42 C and 45 C Built loop between residues 68 D and 71 D 5 chains (507 residues) following loop building 5 chains (507 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4942 restraints for refining 5056 atoms. 819 conditional restraints added. Observations/parameters ratio is 2.13 ------------------------------------------------------ Cycle 41: After refmac, R = 0.2034 (Rfree = 0.000) for 5056 atoms. Found 148 (148 requested) and removed 87 (100 requested) atoms. Cycle 42: After refmac, R = 0.1983 (Rfree = 0.000) for 5108 atoms. Found 110 (145 requested) and removed 19 (101 requested) atoms. Cycle 43: After refmac, R = 0.1922 (Rfree = 0.000) for 5194 atoms. Found 70 (148 requested) and removed 35 (103 requested) atoms. Cycle 44: After refmac, R = 0.1894 (Rfree = 0.000) for 5227 atoms. Found 74 (149 requested) and removed 21 (103 requested) atoms. Cycle 45: After refmac, R = 0.1872 (Rfree = 0.000) for 5275 atoms. Found 68 (150 requested) and removed 39 (104 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 1.92 2.11 NCS extension: 0 residues added, 5306 seeds are put forward Round 1: 506 peptides, 8 chains. Longest chain 124 peptides. Score 0.955 Round 2: 505 peptides, 7 chains. Longest chain 124 peptides. Score 0.957 Round 3: 506 peptides, 10 chains. Longest chain 101 peptides. Score 0.951 Round 4: 506 peptides, 11 chains. Longest chain 92 peptides. Score 0.948 Round 5: 508 peptides, 10 chains. Longest chain 131 peptides. Score 0.951 Taking the results from Round 2 Last building cycle: Chain fragments will be rearranged Chains 9, Residues 498, Estimated correctness of the model 99.8 % 7 chains (496 residues) have been docked in sequence Sequence coverage is 99 % All DUM atoms will be removed Building loops using Loopy2018 Built loop between residues 41 A and 48 A Built loop between residues 65 D and 68 D 6 chains (505 residues) following loop building 5 chains (504 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. Update only waters. 43044 reflections ( 99.90 % complete ) and 4109 restraints for refining 3992 atoms. Observations/parameters ratio is 2.70 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2762 (Rfree = 0.000) for 3992 atoms. Found 111 (111 requested) and removed 0 (111 requested) atoms. Cycle 47: After refmac, R = 0.2543 (Rfree = 0.000) for 3992 atoms. Found 51 (113 requested) and removed 0 (81 requested) atoms. Cycle 48: After refmac, R = 0.2406 (Rfree = 0.000) for 3992 atoms. Found 25 (115 requested) and removed 0 (82 requested) atoms. Cycle 49: After refmac, R = 0.2311 (Rfree = 0.000) for 3992 atoms. Found 11 (115 requested) and removed 1 (82 requested) atoms. Writing output files ... TimeTaking 64.88