null Mon 24 Dec 01:03:40 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir Job ID is set to 1vlm-3.2-parrot-mrncs X-ray data file /users/emra500/scratch/Dataset/mrncsChltofom/1vlm-3.2-parrot-mrncs.mtz Sequence file /users/emra500/scratch/Dataset/mrncsChltofom/1vlm-3.2-parrot-mrncs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir/1vlm-3.2-parrot-mrncs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir/1vlm-3.2-parrot-mrncs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir/1vlm-3.2-parrot-mrncs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Mon 24 Dec 01:03:45 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir/1vlm-3.2-parrot-mrncs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/mrncs/ArpWArp/wArpResults/WorkingDir/1vlm-3.2-parrot-mrncs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 316 and 0 Target number of residues in the AU: 316 Target solvent content: 0.6079 Checking the provided sequence file Detected sequence length: 219 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 2 Adjusted target number of residues: 438 Adjusted target solvent content: 0.46 Input MTZ file: 1vlm-3.2-parrot-mrncs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 18 Cell parameters: 65.648 70.734 93.922 90.000 90.000 90.000 Input sequence file: 1vlm-3.2-parrot-mrncs.fasta_lf Building free atoms model in initial map for 3504 target number of atoms Had to go as low as 0.65 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 3 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 93.922 3.200 Wilson plot Bfac: 55.83 7616 reflections ( 99.75 % complete ) and 0 restraints for refining 3883 atoms. Observations/parameters ratio is 0.49 ------------------------------------------------------ Starting model: R = 0.3059 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.2525 (Rfree = 0.000) for 3883 atoms. Found 34 (34 requested) and removed 87 (17 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 3.05 3.22 Search for helices and strands: 0 residues in 0 chains, 3901 seeds are put forward NCS extension: 0 residues added, 3901 seeds are put forward Round 1: 220 peptides, 38 chains. Longest chain 13 peptides. Score 0.433 Round 2: 258 peptides, 33 chains. Longest chain 21 peptides. Score 0.616 Round 3: 271 peptides, 28 chains. Longest chain 32 peptides. Score 0.701 Round 4: 292 peptides, 34 chains. Longest chain 33 peptides. Score 0.686 Round 5: 271 peptides, 31 chains. Longest chain 31 peptides. Score 0.670 Taking the results from Round 3 Chains 30, Residues 243, Estimated correctness of the model 65.4 % 3 chains (56 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 74 A and 77 A 29 chains (245 residues) following loop building 2 chains (58 residues) in sequence following loop building ------------------------------------------------------ 7616 reflections ( 99.75 % complete ) and 6330 restraints for refining 3198 atoms. 5155 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 1: After refmac, R = 0.2524 (Rfree = 0.000) for 3198 atoms. Found 14 (28 requested) and removed 33 (14 requested) atoms. Cycle 2: After refmac, R = 0.2341 (Rfree = 0.000) for 3148 atoms. Found 9 (27 requested) and removed 21 (14 requested) atoms. Cycle 3: After refmac, R = 0.2282 (Rfree = 0.000) for 3116 atoms. Found 8 (27 requested) and removed 24 (14 requested) atoms. Cycle 4: After refmac, R = 0.2288 (Rfree = 0.000) for 3093 atoms. Found 4 (25 requested) and removed 15 (13 requested) atoms. Cycle 5: After refmac, R = 0.2274 (Rfree = 0.000) for 3080 atoms. Found 1 (25 requested) and removed 18 (13 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 3.03 3.20 Search for helices and strands: 0 residues in 0 chains, 3155 seeds are put forward NCS extension: 12 residues added (9 deleted due to clashes), 3167 seeds are put forward Round 1: 238 peptides, 34 chains. Longest chain 18 peptides. Score 0.547 Round 2: 252 peptides, 28 chains. Longest chain 17 peptides. Score 0.658 Round 3: 257 peptides, 26 chains. Longest chain 18 peptides. Score 0.691 Round 4: 282 peptides, 22 chains. Longest chain 29 peptides. Score 0.776 Round 5: 276 peptides, 23 chains. Longest chain 34 peptides. Score 0.757 Taking the results from Round 4 Chains 23, Residues 260, Estimated correctness of the model 79.6 % 1 chains (22 residues) have been docked in sequence ------------------------------------------------------ 7616 reflections ( 99.75 % complete ) and 6617 restraints for refining 3153 atoms. 5511 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 6: After refmac, R = 0.2346 (Rfree = 0.000) for 3153 atoms. Found 23 (25 requested) and removed 50 (14 requested) atoms. Cycle 7: After refmac, R = 0.2251 (Rfree = 0.000) for 3118 atoms. Found 18 (25 requested) and removed 23 (14 requested) atoms. Cycle 8: After refmac, R = 0.2122 (Rfree = 0.000) for 3108 atoms. Found 15 (23 requested) and removed 22 (13 requested) atoms. Cycle 9: After refmac, R = 0.2060 (Rfree = 0.000) for 3096 atoms. Found 8 (23 requested) and removed 16 (13 requested) atoms. Cycle 10: After refmac, R = 0.1993 (Rfree = 0.000) for 3086 atoms. Found 3 (22 requested) and removed 15 (13 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 3.05 3.22 Search for helices and strands: 0 residues in 0 chains, 3169 seeds are put forward NCS extension: 33 residues added (5 deleted due to clashes), 3202 seeds are put forward Round 1: 249 peptides, 32 chains. Longest chain 32 peptides. Score 0.604 Round 2: 276 peptides, 27 chains. Longest chain 42 peptides. Score 0.721 Round 3: 275 peptides, 32 chains. Longest chain 32 peptides. Score 0.669 Round 4: 278 peptides, 27 chains. Longest chain 40 peptides. Score 0.725 Round 5: 272 peptides, 27 chains. Longest chain 24 peptides. Score 0.713 Taking the results from Round 4 Chains 28, Residues 251, Estimated correctness of the model 70.3 % 3 chains (39 residues) have been docked in sequence Building loops using Loopy2018 28 chains (251 residues) following loop building 3 chains (39 residues) in sequence following loop building ------------------------------------------------------ 7616 reflections ( 99.75 % complete ) and 6590 restraints for refining 3199 atoms. 5448 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 11: After refmac, R = 0.2306 (Rfree = 0.000) for 3199 atoms. Found 22 (22 requested) and removed 29 (14 requested) atoms. Cycle 12: After refmac, R = 0.2204 (Rfree = 0.000) for 3185 atoms. Found 11 (22 requested) and removed 21 (14 requested) atoms. Cycle 13: After refmac, R = 0.2156 (Rfree = 0.000) for 3171 atoms. Found 14 (21 requested) and removed 15 (14 requested) atoms. Cycle 14: After refmac, R = 0.2046 (Rfree = 0.000) for 3164 atoms. Found 4 (21 requested) and removed 15 (14 requested) atoms. Cycle 15: After refmac, R = 0.2069 (Rfree = 0.000) for 3147 atoms. Found 9 (20 requested) and removed 15 (14 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 3.11 3.28 Search for helices and strands: 0 residues in 0 chains, 3229 seeds are put forward NCS extension: 17 residues added (1 deleted due to clashes), 3246 seeds are put forward Round 1: 261 peptides, 32 chains. Longest chain 23 peptides. Score 0.635 Round 2: 275 peptides, 27 chains. Longest chain 28 peptides. Score 0.719 Round 3: 287 peptides, 27 chains. Longest chain 51 peptides. Score 0.742 Round 4: 279 peptides, 25 chains. Longest chain 51 peptides. Score 0.745 Round 5: 281 peptides, 26 chains. Longest chain 46 peptides. Score 0.740 Taking the results from Round 4 Chains 27, Residues 254, Estimated correctness of the model 74.1 % 2 chains (59 residues) have been docked in sequence Building loops using Loopy2018 27 chains (254 residues) following loop building 2 chains (59 residues) in sequence following loop building ------------------------------------------------------ 7616 reflections ( 99.75 % complete ) and 6424 restraints for refining 3199 atoms. 5209 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 16: After refmac, R = 0.2531 (Rfree = 0.000) for 3199 atoms. Found 10 (20 requested) and removed 23 (14 requested) atoms. Cycle 17: After refmac, R = 0.2331 (Rfree = 0.000) for 3175 atoms. Found 5 (19 requested) and removed 18 (14 requested) atoms. Cycle 18: After refmac, R = 0.2261 (Rfree = 0.000) for 3156 atoms. Found 3 (18 requested) and removed 14 (14 requested) atoms. Cycle 19: After refmac, R = 0.2228 (Rfree = 0.000) for 3145 atoms. Found 0 (18 requested) and removed 14 (14 requested) atoms. Cycle 20: After refmac, R = 0.2209 (Rfree = 0.000) for 3130 atoms. Found 6 (17 requested) and removed 14 (14 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 3.09 3.26 Search for helices and strands: 0 residues in 0 chains, 3198 seeds are put forward NCS extension: 43 residues added (4 deleted due to clashes), 3241 seeds are put forward Round 1: 252 peptides, 30 chains. Longest chain 30 peptides. Score 0.635 Round 2: 259 peptides, 26 chains. Longest chain 26 peptides. Score 0.695 Round 3: 264 peptides, 31 chains. Longest chain 23 peptides. Score 0.654 Round 4: 281 peptides, 30 chains. Longest chain 30 peptides. Score 0.702 Round 5: 277 peptides, 29 chains. Longest chain 28 peptides. Score 0.704 Taking the results from Round 5 Chains 30, Residues 248, Estimated correctness of the model 66.0 % 3 chains (30 residues) have been docked in sequence Building loops using Loopy2018 30 chains (248 residues) following loop building 3 chains (30 residues) in sequence following loop building ------------------------------------------------------ 7616 reflections ( 99.75 % complete ) and 6737 restraints for refining 3199 atoms. 5661 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2352 (Rfree = 0.000) for 3199 atoms. Found 16 (17 requested) and removed 22 (14 requested) atoms. Cycle 22: After refmac, R = 0.2256 (Rfree = 0.000) for 3189 atoms. Found 14 (16 requested) and removed 14 (14 requested) atoms. Cycle 23: After refmac, R = 0.2128 (Rfree = 0.000) for 3185 atoms. Found 11 (16 requested) and removed 14 (14 requested) atoms. Cycle 24: After refmac, R = 0.2049 (Rfree = 0.000) for 3178 atoms. Found 5 (15 requested) and removed 14 (14 requested) atoms. Cycle 25: After refmac, R = 0.2040 (Rfree = 0.000) for 3166 atoms. Found 4 (15 requested) and removed 14 (14 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 3.08 3.25 Search for helices and strands: 0 residues in 0 chains, 3261 seeds are put forward NCS extension: 39 residues added (3 deleted due to clashes), 3300 seeds are put forward Round 1: 252 peptides, 32 chains. Longest chain 30 peptides. Score 0.612 Round 2: 269 peptides, 28 chains. Longest chain 24 peptides. Score 0.697 Round 3: 274 peptides, 32 chains. Longest chain 29 peptides. Score 0.666 Round 4: 276 peptides, 29 chains. Longest chain 28 peptides. Score 0.702 Round 5: 265 peptides, 31 chains. Longest chain 36 peptides. Score 0.656 Taking the results from Round 4 Chains 29, Residues 247, Estimated correctness of the model 65.6 % 3 chains (53 residues) have been docked in sequence Building loops using Loopy2018 29 chains (247 residues) following loop building 3 chains (53 residues) in sequence following loop building ------------------------------------------------------ 7616 reflections ( 99.75 % complete ) and 6583 restraints for refining 3199 atoms. 5425 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2402 (Rfree = 0.000) for 3199 atoms. Found 14 (14 requested) and removed 28 (14 requested) atoms. Cycle 27: After refmac, R = 0.2234 (Rfree = 0.000) for 3181 atoms. Found 7 (14 requested) and removed 14 (14 requested) atoms. Cycle 28: After refmac, R = 0.2161 (Rfree = 0.000) for 3174 atoms. Found 8 (14 requested) and removed 14 (14 requested) atoms. Cycle 29: After refmac, R = 0.2142 (Rfree = 0.000) for 3164 atoms. Found 4 (14 requested) and removed 14 (14 requested) atoms. Cycle 30: After refmac, R = 0.2098 (Rfree = 0.000) for 3154 atoms. Found 4 (14 requested) and removed 14 (14 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 3.09 3.26 Search for helices and strands: 0 residues in 0 chains, 3216 seeds are put forward NCS extension: 36 residues added (2 deleted due to clashes), 3252 seeds are put forward Round 1: 243 peptides, 35 chains. Longest chain 20 peptides. Score 0.549 Round 2: 257 peptides, 33 chains. Longest chain 30 peptides. Score 0.613 Round 3: 253 peptides, 27 chains. Longest chain 20 peptides. Score 0.671 Round 4: 253 peptides, 30 chains. Longest chain 29 peptides. Score 0.638 Round 5: 260 peptides, 30 chains. Longest chain 32 peptides. Score 0.655 Taking the results from Round 3 Chains 29, Residues 226, Estimated correctness of the model 58.8 % 2 chains (13 residues) have been docked in sequence ------------------------------------------------------ 7616 reflections ( 99.75 % complete ) and 7044 restraints for refining 3190 atoms. 6120 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2350 (Rfree = 0.000) for 3190 atoms. Found 9 (14 requested) and removed 18 (14 requested) atoms. Cycle 32: After refmac, R = 0.2276 (Rfree = 0.000) for 3178 atoms. Found 7 (14 requested) and removed 14 (14 requested) atoms. Cycle 33: After refmac, R = 0.2231 (Rfree = 0.000) for 3169 atoms. Found 5 (14 requested) and removed 14 (14 requested) atoms. Cycle 34: After refmac, R = 0.2194 (Rfree = 0.000) for 3156 atoms. Found 2 (14 requested) and removed 16 (14 requested) atoms. Cycle 35: After refmac, R = 0.2171 (Rfree = 0.000) for 3140 atoms. Found 4 (14 requested) and removed 14 (14 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 3.07 3.24 Search for helices and strands: 0 residues in 0 chains, 3193 seeds are put forward NCS extension: 9 residues added (0 deleted due to clashes), 3202 seeds are put forward Round 1: 216 peptides, 32 chains. Longest chain 28 peptides. Score 0.507 Round 2: 240 peptides, 28 chains. Longest chain 18 peptides. Score 0.628 Round 3: 262 peptides, 28 chains. Longest chain 23 peptides. Score 0.681 Round 4: 255 peptides, 31 chains. Longest chain 19 peptides. Score 0.631 Round 5: 259 peptides, 28 chains. Longest chain 29 peptides. Score 0.674 Taking the results from Round 3 Chains 28, Residues 234, Estimated correctness of the model 61.0 % 1 chains (11 residues) have been docked in sequence ------------------------------------------------------ 7616 reflections ( 99.75 % complete ) and 6961 restraints for refining 3191 atoms. 6014 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2265 (Rfree = 0.000) for 3191 atoms. Found 14 (14 requested) and removed 17 (14 requested) atoms. Cycle 37: After refmac, R = 0.2113 (Rfree = 0.000) for 3187 atoms. Found 3 (14 requested) and removed 14 (14 requested) atoms. Cycle 38: After refmac, R = 0.2165 (Rfree = 0.000) for 3173 atoms. Found 12 (14 requested) and removed 14 (14 requested) atoms. Cycle 39: After refmac, R = 0.2029 (Rfree = 0.000) for 3169 atoms. Found 5 (14 requested) and removed 14 (14 requested) atoms. Cycle 40: After refmac, R = 0.2047 (Rfree = 0.000) for 3154 atoms. Found 8 (14 requested) and removed 14 (14 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 3.06 3.23 Search for helices and strands: 0 residues in 0 chains, 3238 seeds are put forward NCS extension: 20 residues added (1 deleted due to clashes), 3258 seeds are put forward Round 1: 229 peptides, 35 chains. Longest chain 16 peptides. Score 0.506 Round 2: 244 peptides, 31 chains. Longest chain 18 peptides. Score 0.602 Round 3: 247 peptides, 27 chains. Longest chain 29 peptides. Score 0.657 Round 4: 238 peptides, 30 chains. Longest chain 20 peptides. Score 0.598 Round 5: 239 peptides, 27 chains. Longest chain 25 peptides. Score 0.637 Taking the results from Round 3 Chains 27, Residues 220, Estimated correctness of the model 55.5 % 3 chains (37 residues) have been docked in sequence ------------------------------------------------------ 7616 reflections ( 99.75 % complete ) and 6803 restraints for refining 3198 atoms. 5804 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 41: After refmac, R = 0.2262 (Rfree = 0.000) for 3198 atoms. Found 14 (14 requested) and removed 21 (14 requested) atoms. Cycle 42: After refmac, R = 0.2234 (Rfree = 0.000) for 3182 atoms. Found 14 (14 requested) and removed 17 (14 requested) atoms. Cycle 43: After refmac, R = 0.2188 (Rfree = 0.000) for 3177 atoms. Found 7 (14 requested) and removed 14 (14 requested) atoms. Cycle 44: After refmac, R = 0.2153 (Rfree = 0.000) for 3166 atoms. Found 2 (14 requested) and removed 14 (14 requested) atoms. Cycle 45: After refmac, R = 0.2137 (Rfree = 0.000) for 3153 atoms. Found 7 (14 requested) and removed 14 (14 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 3.09 3.26 Search for helices and strands: 0 residues in 0 chains, 3210 seeds are put forward NCS extension: 38 residues added (4 deleted due to clashes), 3248 seeds are put forward Round 1: 196 peptides, 33 chains. Longest chain 15 peptides. Score 0.425 Round 2: 229 peptides, 30 chains. Longest chain 21 peptides. Score 0.573 Round 3: 238 peptides, 29 chains. Longest chain 30 peptides. Score 0.611 Round 4: 222 peptides, 27 chains. Longest chain 19 peptides. Score 0.592 Round 5: 226 peptides, 28 chains. Longest chain 24 peptides. Score 0.590 Taking the results from Round 3 Last building cycle: Chain fragments will be rearranged Chains 31, Residues 209, Estimated correctness of the model 44.0 % 2 chains (30 residues) have been docked in sequence Sequence coverage is 14 % Consider running further cycles of model building using 1vlm-3_warpNtrace.pdb as input Building loops using Loopy2018 31 chains (209 residues) following loop building 2 chains (30 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. No atom update. 7616 reflections ( 99.75 % complete ) and 6910 restraints for refining 3190 atoms. 5985 conditional restraints added. Observations/parameters ratio is 0.60 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2393 (Rfree = 0.000) for 3190 atoms. Found 0 (14 requested) and removed 4 (14 requested) atoms. Cycle 47: After refmac, R = 0.2324 (Rfree = 0.000) for 3180 atoms. Found 0 (14 requested) and removed 1 (14 requested) atoms. Cycle 48: After refmac, R = 0.2325 (Rfree = 0.000) for 3177 atoms. Found 0 (14 requested) and removed 6 (14 requested) atoms. Cycle 49: After refmac, R = 0.2231 (Rfree = 0.000) for 3168 atoms. Found 0 (14 requested) and removed 2 (14 requested) atoms. Writing output files ... Normal termination of warpNtrace Mon 24 Dec 01:40:10 GMT 2018 Job finished. TimeTaking 36.5 Used memory is bytes: 206664