Tue 25 Dec 19:27:55 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir Job ID is set to 2oh1-3.2-parrot-hancs X-ray data file /users/emra500/scratch/Dataset/hancsChltofom/2oh1-3.2-parrot-hancs.mtz Sequence file /users/emra500/scratch/Dataset/hancsChltofom/2oh1-3.2-parrot-hancs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2oh1-3.2-parrot-hancs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2oh1-3.2-parrot-hancs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2oh1-3.2-parrot-hancs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Tue 25 Dec 19:28:05 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2oh1-3.2-parrot-hancs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2oh1-3.2-parrot-hancs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 546 and 0 Target number of residues in the AU: 546 Target solvent content: 0.6332 Checking the provided sequence file Detected sequence length: 179 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 4 Adjusted target number of residues: 716 Adjusted target solvent content: 0.52 Input MTZ file: 2oh1-3.2-parrot-hancs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 19 Cell parameters: 65.646 99.855 122.905 90.000 90.000 90.000 Input sequence file: 2oh1-3.2-parrot-hancs.fasta_lf Building free atoms model in initial map for 5728 target number of atoms Had to go as low as 0.75 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 3 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 77.501 3.200 Wilson plot Bfac: 68.01 13772 reflections ( 99.14 % complete ) and 0 restraints for refining 6323 atoms. Observations/parameters ratio is 0.54 ------------------------------------------------------ Starting model: R = 0.3090 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.2625 (Rfree = 0.000) for 6323 atoms. Found 41 (56 requested) and removed 57 (28 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 3.25 3.16 Search for helices and strands: 0 residues in 0 chains, 6444 seeds are put forward NCS extension: 0 residues added, 6444 seeds are put forward Round 1: 427 peptides, 70 chains. Longest chain 18 peptides. Score 0.473 Round 2: 475 peptides, 57 chains. Longest chain 25 peptides. Score 0.639 Round 3: 491 peptides, 58 chains. Longest chain 29 peptides. Score 0.654 Round 4: 509 peptides, 53 chains. Longest chain 25 peptides. Score 0.704 Round 5: 504 peptides, 48 chains. Longest chain 23 peptides. Score 0.725 Taking the results from Round 5 Chains 48, Residues 456, Estimated correctness of the model 70.3 % 0 chains (0 residues) have been docked in sequence ------------------------------------------------------ 13772 reflections ( 99.14 % complete ) and 10995 restraints for refining 5227 atoms. 9219 conditional restraints added. Observations/parameters ratio is 0.66 ------------------------------------------------------ Cycle 1: After refmac, R = 0.2406 (Rfree = 0.000) for 5227 atoms. Found 46 (46 requested) and removed 37 (23 requested) atoms. Failed to save intermediate PDB Cycle 2: After refmac, R = 0.2211 (Rfree = 0.000) for 5184 atoms. Found 20 (45 requested) and removed 30 (23 requested) atoms. Cycle 3: After refmac, R = 0.2182 (Rfree = 0.000) for 5145 atoms. Found 24 (44 requested) and removed 32 (23 requested) atoms. Failed to save intermediate PDB Cycle 4: After refmac, R = 0.2144 (Rfree = 0.000) for 5119 atoms. Found 14 (43 requested) and removed 31 (23 requested) atoms. Cycle 5: After refmac, R = 0.2103 (Rfree = 0.000) for 5093 atoms. Found 12 (41 requested) and removed 23 (22 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 3.20 3.12 Search for helices and strands: 0 residues in 0 chains, 5257 seeds are put forward NCS extension: 10 residues added (6 deleted due to clashes), 5267 seeds are put forward Round 1: 463 peptides, 58 chains. Longest chain 19 peptides. Score 0.616 Round 2: 489 peptides, 52 chains. Longest chain 28 peptides. Score 0.686 Round 3: 502 peptides, 50 chains. Longest chain 30 peptides. Score 0.712 Round 4: 533 peptides, 50 chains. Longest chain 43 peptides. Score 0.745 Round 5: 543 peptides, 49 chains. Longest chain 36 peptides. Score 0.759 Taking the results from Round 5 Chains 52, Residues 494, Estimated correctness of the model 76.7 % 5 chains (68 residues) have been docked in sequence Building loops using Loopy2018 52 chains (494 residues) following loop building 5 chains (68 residues) in sequence following loop building ------------------------------------------------------ 13772 reflections ( 99.14 % complete ) and 10359 restraints for refining 5229 atoms. 8157 conditional restraints added. Observations/parameters ratio is 0.66 ------------------------------------------------------ Cycle 6: After refmac, R = 0.2452 (Rfree = 0.000) for 5229 atoms. Found 41 (41 requested) and removed 55 (23 requested) atoms. Failed to save intermediate PDB Cycle 7: After refmac, R = 0.2255 (Rfree = 0.000) for 5189 atoms. Found 34 (40 requested) and removed 29 (23 requested) atoms. Cycle 8: After refmac, R = 0.2169 (Rfree = 0.000) for 5183 atoms. Found 14 (40 requested) and removed 27 (23 requested) atoms. Cycle 9: After refmac, R = 0.2145 (Rfree = 0.000) for 5161 atoms. Found 19 (39 requested) and removed 26 (23 requested) atoms. Failed to save intermediate PDB Cycle 10: After refmac, R = 0.2070 (Rfree = 0.000) for 5147 atoms. Found 4 (38 requested) and removed 25 (23 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 3.23 3.15 Search for helices and strands: 0 residues in 0 chains, 5244 seeds are put forward NCS extension: 21 residues added (9 deleted due to clashes), 5265 seeds are put forward Round 1: 471 peptides, 58 chains. Longest chain 28 peptides. Score 0.627 Round 2: 501 peptides, 51 chains. Longest chain 28 peptides. Score 0.706 Round 3: 492 peptides, 51 chains. Longest chain 22 peptides. Score 0.695 Round 4: 516 peptides, 48 chains. Longest chain 34 peptides. Score 0.737 Round 5: 531 peptides, 46 chains. Longest chain 36 peptides. Score 0.762 Taking the results from Round 5 Chains 50, Residues 485, Estimated correctness of the model 77.2 % 8 chains (121 residues) have been docked in sequence Building loops using Loopy2018 50 chains (485 residues) following loop building 8 chains (121 residues) in sequence following loop building ------------------------------------------------------ Failed to save intermediate PDB 13772 reflections ( 99.14 % complete ) and 9828 restraints for refining 5227 atoms. 7415 conditional restraints added. Observations/parameters ratio is 0.66 ------------------------------------------------------ Cycle 11: After refmac, R = 0.2406 (Rfree = 0.000) for 5227 atoms. Found 37 (37 requested) and removed 43 (23 requested) atoms. Cycle 12: After refmac, R = 0.2229 (Rfree = 0.000) for 5192 atoms. Found 15 (36 requested) and removed 28 (23 requested) atoms. Cycle 13: After refmac, R = 0.2132 (Rfree = 0.000) for 5166 atoms. Found 32 (35 requested) and removed 30 (23 requested) atoms. Failed to save intermediate PDB Cycle 14: After refmac, R = 0.2086 (Rfree = 0.000) for 5161 atoms. Found 9 (34 requested) and removed 25 (23 requested) atoms. Failed to save intermediate PDB Cycle 15: After refmac, R = 0.2112 (Rfree = 0.000) for 5136 atoms. Found 16 (33 requested) and removed 24 (23 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 3.22 3.14 Search for helices and strands: 0 residues in 0 chains, 5251 seeds are put forward NCS extension: 17 residues added (3 deleted due to clashes), 5268 seeds are put forward Round 1: 466 peptides, 57 chains. Longest chain 28 peptides. Score 0.626 Round 2: 509 peptides, 55 chains. Longest chain 30 peptides. Score 0.693 Round 3: 532 peptides, 57 chains. Longest chain 31 peptides. Score 0.709 Round 4: 501 peptides, 46 chains. Longest chain 28 peptides. Score 0.732 Round 5: 512 peptides, 47 chains. Longest chain 32 peptides. Score 0.738 Taking the results from Round 5 Chains 50, Residues 465, Estimated correctness of the model 72.8 % 4 chains (85 residues) have been docked in sequence Building loops using Loopy2018 50 chains (465 residues) following loop building 4 chains (85 residues) in sequence following loop building ------------------------------------------------------ 13772 reflections ( 99.14 % complete ) and 10406 restraints for refining 5229 atoms. 8224 conditional restraints added. Observations/parameters ratio is 0.66 ------------------------------------------------------ Cycle 16: After refmac, R = 0.2487 (Rfree = 0.000) for 5229 atoms. Found 27 (32 requested) and removed 45 (23 requested) atoms. Cycle 17: After refmac, R = 0.2317 (Rfree = 0.000) for 5197 atoms. Found 31 (31 requested) and removed 28 (23 requested) atoms. Cycle 18: After refmac, R = 0.2187 (Rfree = 0.000) for 5192 atoms. Found 5 (30 requested) and removed 29 (23 requested) atoms. Cycle 19: After refmac, R = 0.2130 (Rfree = 0.000) for 5164 atoms. Found 3 (29 requested) and removed 25 (23 requested) atoms. Failed to save intermediate PDB Cycle 20: After refmac, R = 0.2097 (Rfree = 0.000) for 5136 atoms. Found 6 (29 requested) and removed 24 (23 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 3.22 3.14 Search for helices and strands: 0 residues in 0 chains, 5273 seeds are put forward NCS extension: 32 residues added (12 deleted due to clashes), 5305 seeds are put forward Round 1: 439 peptides, 61 chains. Longest chain 22 peptides. Score 0.559 Round 2: 464 peptides, 52 chains. Longest chain 20 peptides. Score 0.654 Round 3: 455 peptides, 50 chains. Longest chain 30 peptides. Score 0.655 Round 4: 462 peptides, 47 chains. Longest chain 37 peptides. Score 0.682 Round 5: 472 peptides, 51 chains. Longest chain 30 peptides. Score 0.671 Taking the results from Round 4 Chains 53, Residues 415, Estimated correctness of the model 61.3 % 7 chains (95 residues) have been docked in sequence ------------------------------------------------------ 13772 reflections ( 99.14 % complete ) and 10309 restraints for refining 5228 atoms. 8285 conditional restraints added. Observations/parameters ratio is 0.66 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2331 (Rfree = 0.000) for 5228 atoms. Found 19 (28 requested) and removed 46 (23 requested) atoms. Cycle 22: After refmac, R = 0.2189 (Rfree = 0.000) for 5190 atoms. Found 6 (28 requested) and removed 25 (23 requested) atoms. Cycle 23: After refmac, R = 0.2186 (Rfree = 0.000) for 5165 atoms. Found 3 (28 requested) and removed 23 (23 requested) atoms. Cycle 24: After refmac, R = 0.2123 (Rfree = 0.000) for 5138 atoms. Found 3 (28 requested) and removed 23 (23 requested) atoms. Failed to save intermediate PDB Cycle 25: After refmac, R = 0.2084 (Rfree = 0.000) for 5113 atoms. Found 3 (27 requested) and removed 22 (22 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 3.22 3.14 Search for helices and strands: 0 residues in 0 chains, 5231 seeds are put forward NCS extension: 11 residues added (5 deleted due to clashes), 5242 seeds are put forward Round 1: 428 peptides, 55 chains. Longest chain 21 peptides. Score 0.584 Round 2: 465 peptides, 48 chains. Longest chain 27 peptides. Score 0.679 Round 3: 469 peptides, 53 chains. Longest chain 33 peptides. Score 0.655 Round 4: 468 peptides, 51 chains. Longest chain 28 peptides. Score 0.666 Round 5: 480 peptides, 47 chains. Longest chain 32 peptides. Score 0.703 Taking the results from Round 5 Chains 54, Residues 433, Estimated correctness of the model 65.8 % 5 chains (76 residues) have been docked in sequence Building loops using Loopy2018 54 chains (433 residues) following loop building 5 chains (76 residues) in sequence following loop building ------------------------------------------------------ 13772 reflections ( 99.14 % complete ) and 10488 restraints for refining 5229 atoms. 8487 conditional restraints added. Observations/parameters ratio is 0.66 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2409 (Rfree = 0.000) for 5229 atoms. Found 18 (28 requested) and removed 42 (23 requested) atoms. Cycle 27: After refmac, R = 0.2225 (Rfree = 0.000) for 5196 atoms. Found 4 (27 requested) and removed 24 (23 requested) atoms. Cycle 28: After refmac, R = 0.2165 (Rfree = 0.000) for 5172 atoms. Found 2 (26 requested) and removed 25 (23 requested) atoms. Cycle 29: After refmac, R = 0.2295 (Rfree = 0.000) for 5143 atoms. Found 18 (25 requested) and removed 26 (23 requested) atoms. Cycle 30: After refmac, R = 0.2073 (Rfree = 0.000) for 5130 atoms. Found 3 (24 requested) and removed 25 (23 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 3.24 3.15 Search for helices and strands: 0 residues in 0 chains, 5235 seeds are put forward NCS extension: 45 residues added (5 deleted due to clashes), 5280 seeds are put forward Round 1: 405 peptides, 55 chains. Longest chain 22 peptides. Score 0.548 Round 2: 421 peptides, 49 chains. Longest chain 29 peptides. Score 0.614 Round 3: 426 peptides, 56 chains. Longest chain 29 peptides. Score 0.574 Round 4: 426 peptides, 48 chains. Longest chain 29 peptides. Score 0.628 Round 5: 438 peptides, 47 chains. Longest chain 23 peptides. Score 0.651 Taking the results from Round 5 Chains 50, Residues 391, Estimated correctness of the model 54.1 % 4 chains (52 residues) have been docked in sequence ------------------------------------------------------ 13772 reflections ( 99.14 % complete ) and 11014 restraints for refining 5229 atoms. 9291 conditional restraints added. Observations/parameters ratio is 0.66 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2242 (Rfree = 0.000) for 5229 atoms. Found 6 (23 requested) and removed 31 (23 requested) atoms. Failed to save intermediate PDB Cycle 32: After refmac, R = 0.2134 (Rfree = 0.000) for 5199 atoms. Found 4 (23 requested) and removed 27 (23 requested) atoms. Cycle 33: After refmac, R = 0.2114 (Rfree = 0.000) for 5168 atoms. Found 6 (23 requested) and removed 24 (23 requested) atoms. Cycle 34: After refmac, R = 0.2091 (Rfree = 0.000) for 5140 atoms. Found 8 (23 requested) and removed 24 (23 requested) atoms. Cycle 35: After refmac, R = 0.2293 (Rfree = 0.000) for 5122 atoms. Found 22 (22 requested) and removed 22 (22 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 3.19 3.11 Search for helices and strands: 0 residues in 0 chains, 5258 seeds are put forward NCS extension: 14 residues added (6 deleted due to clashes), 5272 seeds are put forward Round 1: 363 peptides, 51 chains. Longest chain 19 peptides. Score 0.507 Round 2: 396 peptides, 42 chains. Longest chain 26 peptides. Score 0.625 Round 3: 386 peptides, 48 chains. Longest chain 22 peptides. Score 0.568 Round 4: 394 peptides, 45 chains. Longest chain 21 peptides. Score 0.601 Round 5: 403 peptides, 50 chains. Longest chain 28 peptides. Score 0.580 Taking the results from Round 2 Chains 45, Residues 354, Estimated correctness of the model 47.6 % 6 chains (92 residues) have been docked in sequence ------------------------------------------------------ 13772 reflections ( 99.14 % complete ) and 10537 restraints for refining 5229 atoms. 8745 conditional restraints added. Observations/parameters ratio is 0.66 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2181 (Rfree = 0.000) for 5229 atoms. Found 8 (23 requested) and removed 39 (23 requested) atoms. Failed to save intermediate PDB Cycle 37: After refmac, R = 0.2088 (Rfree = 0.000) for 5191 atoms. Found 17 (23 requested) and removed 24 (23 requested) atoms. Cycle 38: After refmac, R = 0.2010 (Rfree = 0.000) for 5183 atoms. Found 7 (23 requested) and removed 24 (23 requested) atoms. Cycle 39: After refmac, R = 0.1959 (Rfree = 0.000) for 5158 atoms. Found 5 (23 requested) and removed 23 (23 requested) atoms. Cycle 40: After refmac, R = 0.1908 (Rfree = 0.000) for 5136 atoms. Found 2 (23 requested) and removed 23 (23 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 3.21 3.13 Search for helices and strands: 0 residues in 0 chains, 5229 seeds are put forward NCS extension: 19 residues added (11 deleted due to clashes), 5248 seeds are put forward Round 1: 360 peptides, 51 chains. Longest chain 20 peptides. Score 0.502 Round 2: 389 peptides, 50 chains. Longest chain 22 peptides. Score 0.558 Round 3: 384 peptides, 54 chains. Longest chain 22 peptides. Score 0.520 Round 4: 389 peptides, 48 chains. Longest chain 24 peptides. Score 0.573 Round 5: 380 peptides, 45 chains. Longest chain 24 peptides. Score 0.580 Taking the results from Round 5 Chains 52, Residues 335, Estimated correctness of the model 35.6 % 6 chains (70 residues) have been docked in sequence ------------------------------------------------------ 13772 reflections ( 99.14 % complete ) and 10918 restraints for refining 5229 atoms. 9319 conditional restraints added. Observations/parameters ratio is 0.66 ------------------------------------------------------ Cycle 41: After refmac, R = 0.2144 (Rfree = 0.000) for 5229 atoms. Found 11 (23 requested) and removed 31 (23 requested) atoms. Cycle 42: After refmac, R = 0.2059 (Rfree = 0.000) for 5196 atoms. Found 14 (23 requested) and removed 26 (23 requested) atoms. Cycle 43: After refmac, R = 0.2006 (Rfree = 0.000) for 5181 atoms. Found 4 (23 requested) and removed 23 (23 requested) atoms. Cycle 44: After refmac, R = 0.1989 (Rfree = 0.000) for 5159 atoms. Found 5 (23 requested) and removed 23 (23 requested) atoms. Cycle 45: After refmac, R = 0.2188 (Rfree = 0.000) for 5136 atoms. Found 19 (23 requested) and removed 28 (23 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 3.17 3.09 Search for helices and strands: 0 residues in 0 chains, 5267 seeds are put forward NCS extension: 20 residues added (1 deleted due to clashes), 5287 seeds are put forward Round 1: 345 peptides, 53 chains. Longest chain 19 peptides. Score 0.458 Round 2: 384 peptides, 49 chains. Longest chain 24 peptides. Score 0.557 Round 3: 389 peptides, 50 chains. Longest chain 26 peptides. Score 0.558 Round 4: 383 peptides, 48 chains. Longest chain 27 peptides. Score 0.563 Round 5: 392 peptides, 48 chains. Longest chain 28 peptides. Score 0.577 Taking the results from Round 5 Last building cycle: Chain fragments will be rearranged Chains 49, Residues 344, Estimated correctness of the model 34.7 % 5 chains (66 residues) have been docked in sequence Sequence coverage is 19 % Consider running further cycles of model building using 2oh1-3_warpNtrace.pdb as input Building loops using Loopy2018 49 chains (344 residues) following loop building 5 chains (66 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. No atom update. 13772 reflections ( 99.14 % complete ) and 10889 restraints for refining 5229 atoms. 9281 conditional restraints added. Observations/parameters ratio is 0.66 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2148 (Rfree = 0.000) for 5229 atoms. Found 0 (23 requested) and removed 23 (23 requested) atoms. Cycle 47: After refmac, R = 0.2249 (Rfree = 0.000) for 5202 atoms. Found 0 (23 requested) and removed 23 (23 requested) atoms. Cycle 48: After refmac, R = 0.1986 (Rfree = 0.000) for 5171 atoms. Found 0 (23 requested) and removed 20 (23 requested) atoms. Cycle 49: After refmac, R = 0.2253 (Rfree = 0.000) for 5147 atoms. Found 0 (23 requested) and removed 23 (23 requested) atoms. Writing output files ... Failed to save intermediate PDB TimeTaking 82.23