Tue 25 Dec 19:27:57 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir Job ID is set to 2od6-3.2-parrot-hancs X-ray data file /users/emra500/scratch/Dataset/hancsChltofom/2od6-3.2-parrot-hancs.mtz Sequence file /users/emra500/scratch/Dataset/hancsChltofom/2od6-3.2-parrot-hancs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2od6-3.2-parrot-hancs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2od6-3.2-parrot-hancs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2od6-3.2-parrot-hancs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Tue 25 Dec 19:28:06 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2od6-3.2-parrot-hancs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2od6-3.2-parrot-hancs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 333 and 0 Target number of residues in the AU: 333 Target solvent content: 0.6318 Checking the provided sequence file Detected sequence length: 110 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 4 Adjusted target number of residues: 440 Adjusted target solvent content: 0.51 Input MTZ file: 2od6-3.2-parrot-hancs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 19 Cell parameters: 36.403 92.834 144.837 90.000 90.000 90.000 Input sequence file: 2od6-3.2-parrot-hancs.fasta_lf Building free atoms model in initial map for 3520 target number of atoms Had to go as low as 0.70 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 3 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 29.066 3.200 Wilson plot Bfac: 67.36 8570 reflections ( 99.12 % complete ) and 0 restraints for refining 3880 atoms. Observations/parameters ratio is 0.55 ------------------------------------------------------ Starting model: R = 0.3303 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.3183 (Rfree = 0.000) for 3880 atoms. Found 27 (34 requested) and removed 30 (17 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 3.23 3.19 Search for helices and strands: 0 residues in 0 chains, 3984 seeds are put forward NCS extension: 0 residues added, 3984 seeds are put forward Round 1: 220 peptides, 37 chains. Longest chain 11 peptides. Score 0.425 Round 2: 279 peptides, 41 chains. Longest chain 22 peptides. Score 0.546 Round 3: 289 peptides, 36 chains. Longest chain 28 peptides. Score 0.626 Round 4: 298 peptides, 36 chains. Longest chain 29 peptides. Score 0.646 Round 5: 316 peptides, 33 chains. Longest chain 33 peptides. Score 0.710 Taking the results from Round 5 Chains 34, Residues 283, Estimated correctness of the model 67.3 % 3 chains (76 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 35 B and 38 B 34 chains (283 residues) following loop building 2 chains (78 residues) in sequence following loop building ------------------------------------------------------ 8570 reflections ( 99.12 % complete ) and 5992 restraints for refining 3211 atoms. 4552 conditional restraints added. Observations/parameters ratio is 0.67 ------------------------------------------------------ Cycle 1: After refmac, R = 0.2998 (Rfree = 0.000) for 3211 atoms. Found 16 (28 requested) and removed 26 (14 requested) atoms. Cycle 2: After refmac, R = 0.2795 (Rfree = 0.000) for 3141 atoms. Found 5 (27 requested) and removed 20 (14 requested) atoms. Cycle 3: After refmac, R = 0.2585 (Rfree = 0.000) for 3109 atoms. Found 10 (27 requested) and removed 17 (14 requested) atoms. Cycle 4: After refmac, R = 0.2596 (Rfree = 0.000) for 3097 atoms. Found 3 (25 requested) and removed 20 (13 requested) atoms. Failed to save intermediate PDB Cycle 5: After refmac, R = 0.2553 (Rfree = 0.000) for 3071 atoms. Found 6 (25 requested) and removed 17 (13 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 3.23 3.19 Search for helices and strands: 0 residues in 0 chains, 3201 seeds are put forward NCS extension: 56 residues added (30 deleted due to clashes), 3257 seeds are put forward Round 1: 283 peptides, 34 chains. Longest chain 17 peptides. Score 0.633 Round 2: 312 peptides, 31 chains. Longest chain 27 peptides. Score 0.719 Round 3: 324 peptides, 33 chains. Longest chain 28 peptides. Score 0.724 Round 4: 319 peptides, 29 chains. Longest chain 36 peptides. Score 0.748 Round 5: 317 peptides, 31 chains. Longest chain 38 peptides. Score 0.728 Taking the results from Round 4 Chains 30, Residues 290, Estimated correctness of the model 74.7 % 3 chains (75 residues) have been docked in sequence Building loops using Loopy2018 30 chains (290 residues) following loop building 3 chains (75 residues) in sequence following loop building ------------------------------------------------------ Failed to save intermediate PDB 8570 reflections ( 99.12 % complete ) and 5974 restraints for refining 3213 atoms. 4504 conditional restraints added. Observations/parameters ratio is 0.67 ------------------------------------------------------ Cycle 6: After refmac, R = 0.2871 (Rfree = 0.000) for 3213 atoms. Found 16 (25 requested) and removed 27 (14 requested) atoms. Cycle 7: After refmac, R = 0.2657 (Rfree = 0.000) for 3190 atoms. Found 7 (25 requested) and removed 15 (14 requested) atoms. Failed to save intermediate PDB Cycle 8: After refmac, R = 0.2608 (Rfree = 0.000) for 3178 atoms. Found 6 (24 requested) and removed 24 (14 requested) atoms. Cycle 9: After refmac, R = 0.2739 (Rfree = 0.000) for 3156 atoms. Found 10 (24 requested) and removed 16 (14 requested) atoms. Failed to save intermediate PDB Cycle 10: After refmac, R = 0.2454 (Rfree = 0.000) for 3141 atoms. Found 6 (23 requested) and removed 16 (14 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 3.25 3.21 Search for helices and strands: 0 residues in 0 chains, 3216 seeds are put forward NCS extension: 17 residues added (19 deleted due to clashes), 3233 seeds are put forward Round 1: 298 peptides, 35 chains. Longest chain 24 peptides. Score 0.656 Round 2: 307 peptides, 31 chains. Longest chain 24 peptides. Score 0.710 Round 3: 311 peptides, 32 chains. Longest chain 24 peptides. Score 0.709 Round 4: 307 peptides, 33 chains. Longest chain 29 peptides. Score 0.693 Round 5: 319 peptides, 33 chains. Longest chain 28 peptides. Score 0.715 Taking the results from Round 5 Chains 34, Residues 286, Estimated correctness of the model 68.3 % 2 chains (42 residues) have been docked in sequence Building loops using Loopy2018 34 chains (286 residues) following loop building 2 chains (42 residues) in sequence following loop building ------------------------------------------------------ 8570 reflections ( 99.12 % complete ) and 6309 restraints for refining 3212 atoms. 5009 conditional restraints added. Observations/parameters ratio is 0.67 ------------------------------------------------------ Cycle 11: After refmac, R = 0.2914 (Rfree = 0.000) for 3212 atoms. Found 19 (22 requested) and removed 19 (14 requested) atoms. Failed to save intermediate PDB Cycle 12: After refmac, R = 0.2637 (Rfree = 0.000) for 3192 atoms. Found 10 (22 requested) and removed 17 (14 requested) atoms. Failed to save intermediate PDB Cycle 13: After refmac, R = 0.2663 (Rfree = 0.000) for 3181 atoms. Found 11 (21 requested) and removed 20 (14 requested) atoms. Failed to save intermediate PDB Cycle 14: After refmac, R = 0.2628 (Rfree = 0.000) for 3167 atoms. Found 13 (21 requested) and removed 18 (14 requested) atoms. Failed to save intermediate PDB Cycle 15: After refmac, R = 0.2545 (Rfree = 0.000) for 3161 atoms. Found 9 (20 requested) and removed 18 (14 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 3.25 3.21 Search for helices and strands: 0 residues in 0 chains, 3263 seeds are put forward NCS extension: 18 residues added (9 deleted due to clashes), 3281 seeds are put forward Round 1: 277 peptides, 33 chains. Longest chain 18 peptides. Score 0.629 Round 2: 290 peptides, 34 chains. Longest chain 24 peptides. Score 0.648 Round 3: 298 peptides, 34 chains. Longest chain 20 peptides. Score 0.665 Round 4: 295 peptides, 31 chains. Longest chain 30 peptides. Score 0.687 Round 5: 295 peptides, 28 chains. Longest chain 31 peptides. Score 0.714 Taking the results from Round 5 Chains 28, Residues 267, Estimated correctness of the model 68.1 % 1 chains (30 residues) have been docked in sequence ------------------------------------------------------ Failed to save intermediate PDB 8570 reflections ( 99.12 % complete ) and 6617 restraints for refining 3212 atoms. 5437 conditional restraints added. Observations/parameters ratio is 0.67 ------------------------------------------------------ Cycle 16: After refmac, R = 0.2733 (Rfree = 0.000) for 3212 atoms. Found 13 (20 requested) and removed 18 (14 requested) atoms. Cycle 17: After refmac, R = 0.2619 (Rfree = 0.000) for 3202 atoms. Found 6 (19 requested) and removed 16 (14 requested) atoms. Failed to save intermediate PDB Cycle 18: After refmac, R = 0.2603 (Rfree = 0.000) for 3185 atoms. Found 11 (18 requested) and removed 18 (14 requested) atoms. Failed to save intermediate PDB Cycle 19: After refmac, R = 0.2516 (Rfree = 0.000) for 3176 atoms. Found 7 (18 requested) and removed 15 (14 requested) atoms. Cycle 20: After refmac, R = 0.2400 (Rfree = 0.000) for 3166 atoms. Found 7 (17 requested) and removed 15 (14 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 3.24 3.20 Search for helices and strands: 0 residues in 0 chains, 3265 seeds are put forward NCS extension: 27 residues added (6 deleted due to clashes), 3292 seeds are put forward Round 1: 271 peptides, 36 chains. Longest chain 21 peptides. Score 0.583 Round 2: 288 peptides, 29 chains. Longest chain 27 peptides. Score 0.692 Round 3: 293 peptides, 33 chains. Longest chain 27 peptides. Score 0.664 Round 4: 296 peptides, 34 chains. Longest chain 23 peptides. Score 0.661 Round 5: 282 peptides, 28 chains. Longest chain 26 peptides. Score 0.689 Taking the results from Round 2 Chains 29, Residues 259, Estimated correctness of the model 63.5 % 0 chains (0 residues) have been docked in sequence ------------------------------------------------------ Failed to save intermediate PDB 8570 reflections ( 99.12 % complete ) and 6828 restraints for refining 3212 atoms. 5821 conditional restraints added. Observations/parameters ratio is 0.67 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2533 (Rfree = 0.000) for 3212 atoms. Found 11 (17 requested) and removed 17 (14 requested) atoms. Cycle 22: After refmac, R = 0.2551 (Rfree = 0.000) for 3204 atoms. Found 5 (17 requested) and removed 14 (14 requested) atoms. Failed to save intermediate PDB Cycle 23: After refmac, R = 0.2355 (Rfree = 0.000) for 3192 atoms. Found 2 (17 requested) and removed 14 (14 requested) atoms. Cycle 24: After refmac, R = 0.2296 (Rfree = 0.000) for 3178 atoms. Found 5 (17 requested) and removed 14 (14 requested) atoms. Cycle 25: After refmac, R = 0.2567 (Rfree = 0.000) for 3168 atoms. Found 15 (17 requested) and removed 14 (14 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 3.27 3.23 Search for helices and strands: 0 residues in 0 chains, 3261 seeds are put forward NCS extension: 19 residues added (14 deleted due to clashes), 3280 seeds are put forward Round 1: 247 peptides, 35 chains. Longest chain 17 peptides. Score 0.531 Round 2: 263 peptides, 31 chains. Longest chain 23 peptides. Score 0.618 Round 3: 279 peptides, 31 chains. Longest chain 25 peptides. Score 0.654 Round 4: 277 peptides, 34 chains. Longest chain 21 peptides. Score 0.619 Round 5: 261 peptides, 32 chains. Longest chain 22 peptides. Score 0.602 Taking the results from Round 3 Chains 33, Residues 248, Estimated correctness of the model 54.8 % 1 chains (20 residues) have been docked in sequence ------------------------------------------------------ 8570 reflections ( 99.12 % complete ) and 6702 restraints for refining 3213 atoms. 5655 conditional restraints added. Observations/parameters ratio is 0.67 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2540 (Rfree = 0.000) for 3213 atoms. Found 8 (17 requested) and removed 16 (14 requested) atoms. Failed to save intermediate PDB Cycle 27: After refmac, R = 0.2474 (Rfree = 0.000) for 3194 atoms. Found 10 (17 requested) and removed 17 (14 requested) atoms. Cycle 28: After refmac, R = 0.2608 (Rfree = 0.000) for 3184 atoms. Found 10 (17 requested) and removed 14 (14 requested) atoms. Cycle 29: After refmac, R = 0.2545 (Rfree = 0.000) for 3173 atoms. Found 9 (17 requested) and removed 18 (14 requested) atoms. Cycle 30: After refmac, R = 0.2906 (Rfree = 0.000) for 3161 atoms. Found 17 (17 requested) and removed 20 (14 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 3.23 3.19 Search for helices and strands: 0 residues in 0 chains, 3263 seeds are put forward NCS extension: 17 residues added (10 deleted due to clashes), 3280 seeds are put forward Round 1: 251 peptides, 33 chains. Longest chain 21 peptides. Score 0.566 Round 2: 277 peptides, 32 chains. Longest chain 20 peptides. Score 0.640 Round 3: 273 peptides, 30 chains. Longest chain 22 peptides. Score 0.651 Round 4: 281 peptides, 32 chains. Longest chain 21 peptides. Score 0.648 Round 5: 277 peptides, 28 chains. Longest chain 23 peptides. Score 0.679 Taking the results from Round 5 Chains 28, Residues 249, Estimated correctness of the model 60.6 % 1 chains (12 residues) have been docked in sequence ------------------------------------------------------ 8570 reflections ( 99.12 % complete ) and 6708 restraints for refining 3213 atoms. 5687 conditional restraints added. Observations/parameters ratio is 0.67 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2516 (Rfree = 0.000) for 3213 atoms. Found 15 (17 requested) and removed 20 (14 requested) atoms. Failed to save intermediate PDB Cycle 32: After refmac, R = 0.2375 (Rfree = 0.000) for 3196 atoms. Found 15 (17 requested) and removed 14 (14 requested) atoms. Cycle 33: After refmac, R = 0.2265 (Rfree = 0.000) for 3189 atoms. Found 7 (17 requested) and removed 14 (14 requested) atoms. Cycle 34: After refmac, R = 0.2255 (Rfree = 0.000) for 3177 atoms. Found 8 (17 requested) and removed 14 (14 requested) atoms. Failed to save intermediate PDB Cycle 35: After refmac, R = 0.2325 (Rfree = 0.000) for 3169 atoms. Found 17 (17 requested) and removed 15 (14 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 3.26 3.22 Search for helices and strands: 0 residues in 0 chains, 3297 seeds are put forward NCS extension: 18 residues added (1 deleted due to clashes), 3315 seeds are put forward Round 1: 229 peptides, 33 chains. Longest chain 18 peptides. Score 0.505 Round 2: 267 peptides, 34 chains. Longest chain 18 peptides. Score 0.595 Round 3: 283 peptides, 34 chains. Longest chain 18 peptides. Score 0.633 Round 4: 263 peptides, 33 chains. Longest chain 21 peptides. Score 0.596 Round 5: 269 peptides, 32 chains. Longest chain 22 peptides. Score 0.621 Taking the results from Round 3 Chains 35, Residues 249, Estimated correctness of the model 49.7 % 1 chains (11 residues) have been docked in sequence ------------------------------------------------------ Failed to save intermediate PDB 8570 reflections ( 99.12 % complete ) and 6681 restraints for refining 3213 atoms. 5672 conditional restraints added. Observations/parameters ratio is 0.67 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2353 (Rfree = 0.000) for 3213 atoms. Found 11 (17 requested) and removed 16 (14 requested) atoms. Cycle 37: After refmac, R = 0.2288 (Rfree = 0.000) for 3202 atoms. Found 7 (17 requested) and removed 16 (14 requested) atoms. Failed to save intermediate PDB Cycle 38: After refmac, R = 0.2294 (Rfree = 0.000) for 3191 atoms. Found 7 (17 requested) and removed 14 (14 requested) atoms. Cycle 39: After refmac, R = 0.2282 (Rfree = 0.000) for 3183 atoms. Found 3 (17 requested) and removed 14 (14 requested) atoms. Cycle 40: After refmac, R = 0.2238 (Rfree = 0.000) for 3172 atoms. Found 1 (17 requested) and removed 14 (14 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 3.25 3.21 Search for helices and strands: 0 residues in 0 chains, 3247 seeds are put forward NCS extension: 15 residues added (1 deleted due to clashes), 3262 seeds are put forward Round 1: 222 peptides, 34 chains. Longest chain 19 peptides. Score 0.472 Round 2: 241 peptides, 31 chains. Longest chain 16 peptides. Score 0.563 Round 3: 244 peptides, 30 chains. Longest chain 21 peptides. Score 0.583 Round 4: 232 peptides, 26 chains. Longest chain 22 peptides. Score 0.599 Round 5: 245 peptides, 29 chains. Longest chain 22 peptides. Score 0.597 Taking the results from Round 4 Chains 26, Residues 206, Estimated correctness of the model 40.8 % 0 chains (0 residues) have been docked in sequence ------------------------------------------------------ 8570 reflections ( 99.12 % complete ) and 7028 restraints for refining 3213 atoms. 6230 conditional restraints added. Observations/parameters ratio is 0.67 ------------------------------------------------------ Cycle 41: After refmac, R = 0.2347 (Rfree = 0.000) for 3213 atoms. Found 14 (17 requested) and removed 14 (14 requested) atoms. Cycle 42: After refmac, R = 0.2324 (Rfree = 0.000) for 3212 atoms. Found 7 (17 requested) and removed 14 (14 requested) atoms. Cycle 43: After refmac, R = 0.2170 (Rfree = 0.000) for 3205 atoms. Found 7 (17 requested) and removed 14 (14 requested) atoms. Cycle 44: After refmac, R = 0.2250 (Rfree = 0.000) for 3197 atoms. Found 10 (17 requested) and removed 14 (14 requested) atoms. Cycle 45: After refmac, R = 0.2080 (Rfree = 0.000) for 3191 atoms. Found 1 (17 requested) and removed 14 (14 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 3.28 3.24 Search for helices and strands: 0 residues in 0 chains, 3258 seeds are put forward NCS extension: 16 residues added (0 deleted due to clashes), 3274 seeds are put forward Round 1: 227 peptides, 35 chains. Longest chain 17 peptides. Score 0.474 Round 2: 239 peptides, 27 chains. Longest chain 19 peptides. Score 0.604 Round 3: 243 peptides, 27 chains. Longest chain 20 peptides. Score 0.614 Round 4: 245 peptides, 29 chains. Longest chain 27 peptides. Score 0.597 Round 5: 241 peptides, 28 chains. Longest chain 24 peptides. Score 0.598 Taking the results from Round 3 Last building cycle: Chain fragments will be rearranged Chains 27, Residues 216, Estimated correctness of the model 44.8 % 0 chains (0 residues) have been docked in sequence Sequence coverage is 0 % Consider running further cycles of model building using 2od6-3_warpNtrace.pdb as input ------------------------------------------------------ ---> Final restrained refinement block. No atom update. 8570 reflections ( 99.12 % complete ) and 6934 restraints for refining 3213 atoms. 6097 conditional restraints added. Observations/parameters ratio is 0.67 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2349 (Rfree = 0.000) for 3213 atoms. Found 0 (17 requested) and removed 14 (14 requested) atoms. Cycle 47: After refmac, R = 0.2370 (Rfree = 0.000) for 3194 atoms. Found 0 (17 requested) and removed 9 (14 requested) atoms. Cycle 48: After refmac, R = 0.2211 (Rfree = 0.000) for 3184 atoms. Found 0 (17 requested) and removed 4 (14 requested) atoms. Cycle 49: After refmac, R = 0.2278 (Rfree = 0.000) for 3177 atoms. Found 0 (17 requested) and removed 7 (14 requested) atoms. Writing output files ... Failed to save intermediate PDB TimeTaking 60.37