Tue 25 Dec 19:27:57 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir Job ID is set to 2od6-1.8-parrot-hancs X-ray data file /users/emra500/scratch/Dataset/hancsChltofom/2od6-1.8-parrot-hancs.mtz Sequence file /users/emra500/scratch/Dataset/hancsChltofom/2od6-1.8-parrot-hancs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2od6-1.8-parrot-hancs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2od6-1.8-parrot-hancs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2od6-1.8-parrot-hancs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Tue 25 Dec 19:28:07 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2od6-1.8-parrot-hancs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2od6-1.8-parrot-hancs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 437 and 0 Target number of residues in the AU: 437 Target solvent content: 0.5168 Checking the provided sequence file Detected sequence length: 110 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 4 Adjusted target number of residues: 440 Adjusted target solvent content: 0.51 Input MTZ file: 2od6-1.8-parrot-hancs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 19 Cell parameters: 36.403 92.834 144.837 90.000 90.000 90.000 Input sequence file: 2od6-1.8-parrot-hancs.fasta_lf Building free atoms model in initial map for 3520 target number of atoms Had to go as low as 0.45 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 1 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 29.066 1.849 Wilson plot Bfac: 27.04 42951 reflections ( 99.78 % complete ) and 0 restraints for refining 3911 atoms. Observations/parameters ratio is 2.75 ------------------------------------------------------ Starting model: R = 0.3368 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.2892 (Rfree = 0.000) for 3911 atoms. Found 105 (167 requested) and removed 85 (83 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 2.13 2.10 NCS extension: 0 residues added, 3931 seeds are put forward Round 1: 335 peptides, 28 chains. Longest chain 45 peptides. Score 0.780 Round 2: 369 peptides, 23 chains. Longest chain 54 peptides. Score 0.851 Round 3: 399 peptides, 17 chains. Longest chain 75 peptides. Score 0.903 Round 4: 412 peptides, 7 chains. Longest chain 105 peptides. Score 0.945 Round 5: 405 peptides, 13 chains. Longest chain 81 peptides. Score 0.921 Taking the results from Round 4 Chains 8, Residues 405, Estimated correctness of the model 99.7 % 7 chains (391 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 18 A and 21 A 7 chains (407 residues) following loop building 6 chains (393 residues) in sequence following loop building ------------------------------------------------------ 42951 reflections ( 99.78 % complete ) and 3968 restraints for refining 3800 atoms. 627 conditional restraints added. Observations/parameters ratio is 2.83 ------------------------------------------------------ Cycle 1: After refmac, R = 0.2901 (Rfree = 0.000) for 3800 atoms. Found 112 (162 requested) and removed 87 (81 requested) atoms. Cycle 2: After refmac, R = 0.2647 (Rfree = 0.000) for 3808 atoms. Found 92 (160 requested) and removed 58 (81 requested) atoms. Failed to save intermediate PDB Cycle 3: After refmac, R = 0.2522 (Rfree = 0.000) for 3825 atoms. Found 90 (157 requested) and removed 32 (82 requested) atoms. Cycle 4: After refmac, R = 0.2416 (Rfree = 0.000) for 3871 atoms. Found 77 (159 requested) and removed 25 (83 requested) atoms. Cycle 5: After refmac, R = 0.2338 (Rfree = 0.000) for 3911 atoms. Found 70 (161 requested) and removed 40 (84 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 2.13 2.10 NCS extension: 13 residues added (37 deleted due to clashes), 3956 seeds are put forward Round 1: 408 peptides, 5 chains. Longest chain 105 peptides. Score 0.949 Round 2: 409 peptides, 6 chains. Longest chain 106 peptides. Score 0.947 Round 3: 404 peptides, 10 chains. Longest chain 107 peptides. Score 0.931 Round 4: 409 peptides, 9 chains. Longest chain 107 peptides. Score 0.937 Round 5: 405 peptides, 11 chains. Longest chain 107 peptides. Score 0.928 Taking the results from Round 1 Chains 5, Residues 403, Estimated correctness of the model 99.8 % 5 chains (403 residues) have been docked in sequence Building loops using Loopy2018 5 chains (403 residues) following loop building 5 chains (403 residues) in sequence following loop building ------------------------------------------------------ Failed to save intermediate PDB 42951 reflections ( 99.78 % complete ) and 3935 restraints for refining 3874 atoms. 567 conditional restraints added. Observations/parameters ratio is 2.77 ------------------------------------------------------ Cycle 6: After refmac, R = 0.2449 (Rfree = 0.000) for 3874 atoms. Found 106 (155 requested) and removed 84 (82 requested) atoms. Cycle 7: After refmac, R = 0.2350 (Rfree = 0.000) for 3892 atoms. Found 92 (153 requested) and removed 31 (83 requested) atoms. Cycle 8: After refmac, R = 0.2273 (Rfree = 0.000) for 3947 atoms. Found 65 (155 requested) and removed 20 (84 requested) atoms. Cycle 9: After refmac, R = 0.2217 (Rfree = 0.000) for 3987 atoms. Found 52 (157 requested) and removed 30 (85 requested) atoms. Failed to save intermediate PDB Cycle 10: After refmac, R = 0.2200 (Rfree = 0.000) for 4001 atoms. Found 64 (154 requested) and removed 22 (85 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 2.13 2.10 NCS extension: 13 residues added (13 deleted due to clashes), 4056 seeds are put forward Round 1: 414 peptides, 7 chains. Longest chain 107 peptides. Score 0.945 Round 2: 414 peptides, 6 chains. Longest chain 106 peptides. Score 0.948 Round 3: 410 peptides, 10 chains. Longest chain 105 peptides. Score 0.934 Round 4: 410 peptides, 10 chains. Longest chain 105 peptides. Score 0.934 Round 5: 411 peptides, 10 chains. Longest chain 107 peptides. Score 0.935 Taking the results from Round 2 Chains 6, Residues 408, Estimated correctness of the model 99.8 % 5 chains (405 residues) have been docked in sequence Building loops using Loopy2018 6 chains (408 residues) following loop building 5 chains (405 residues) in sequence following loop building ------------------------------------------------------ 42951 reflections ( 99.78 % complete ) and 3916 restraints for refining 3917 atoms. 520 conditional restraints added. Observations/parameters ratio is 2.74 ------------------------------------------------------ Cycle 11: After refmac, R = 0.2366 (Rfree = 0.000) for 3917 atoms. Found 113 (150 requested) and removed 71 (83 requested) atoms. Cycle 12: After refmac, R = 0.2260 (Rfree = 0.000) for 3953 atoms. Found 81 (149 requested) and removed 44 (84 requested) atoms. Cycle 13: After refmac, R = 0.2203 (Rfree = 0.000) for 3983 atoms. Found 93 (146 requested) and removed 23 (85 requested) atoms. Cycle 14: After refmac, R = 0.2152 (Rfree = 0.000) for 4047 atoms. Found 69 (149 requested) and removed 28 (86 requested) atoms. Cycle 15: After refmac, R = 0.2135 (Rfree = 0.000) for 4078 atoms. Found 72 (150 requested) and removed 31 (87 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 2.13 2.10 NCS extension: 13 residues added (13 deleted due to clashes), 4132 seeds are put forward Round 1: 411 peptides, 9 chains. Longest chain 107 peptides. Score 0.938 Round 2: 404 peptides, 10 chains. Longest chain 81 peptides. Score 0.931 Round 3: 408 peptides, 6 chains. Longest chain 107 peptides. Score 0.946 Round 4: 407 peptides, 11 chains. Longest chain 107 peptides. Score 0.929 Round 5: 409 peptides, 9 chains. Longest chain 106 peptides. Score 0.937 Taking the results from Round 3 Chains 6, Residues 402, Estimated correctness of the model 99.7 % 6 chains (402 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 48 D and 53 D 5 chains (406 residues) following loop building 5 chains (406 residues) in sequence following loop building ------------------------------------------------------ 42951 reflections ( 99.78 % complete ) and 3910 restraints for refining 3974 atoms. 516 conditional restraints added. Observations/parameters ratio is 2.70 ------------------------------------------------------ Cycle 16: After refmac, R = 0.2329 (Rfree = 0.000) for 3974 atoms. Found 122 (146 requested) and removed 78 (85 requested) atoms. Cycle 17: After refmac, R = 0.2233 (Rfree = 0.000) for 4011 atoms. Found 83 (144 requested) and removed 34 (86 requested) atoms. Failed to save intermediate PDB Cycle 18: After refmac, R = 0.2153 (Rfree = 0.000) for 4055 atoms. Found 71 (145 requested) and removed 14 (86 requested) atoms. Failed to save intermediate PDB Cycle 19: After refmac, R = 0.2118 (Rfree = 0.000) for 4104 atoms. Found 69 (148 requested) and removed 34 (88 requested) atoms. Cycle 20: After refmac, R = 0.2082 (Rfree = 0.000) for 4130 atoms. Found 69 (149 requested) and removed 44 (88 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 2.12 2.09 NCS extension: 10 residues added (16 deleted due to clashes), 4165 seeds are put forward Round 1: 414 peptides, 7 chains. Longest chain 107 peptides. Score 0.945 Round 2: 415 peptides, 7 chains. Longest chain 107 peptides. Score 0.946 Round 3: 410 peptides, 12 chains. Longest chain 90 peptides. Score 0.927 Round 4: 417 peptides, 9 chains. Longest chain 107 peptides. Score 0.940 Round 5: 419 peptides, 8 chains. Longest chain 107 peptides. Score 0.944 Taking the results from Round 2 Chains 7, Residues 408, Estimated correctness of the model 99.7 % 6 chains (405 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 31 D and 34 D 6 chains (410 residues) following loop building 5 chains (407 residues) in sequence following loop building ------------------------------------------------------ Failed to save intermediate PDB 42951 reflections ( 99.78 % complete ) and 3927 restraints for refining 3993 atoms. 514 conditional restraints added. Observations/parameters ratio is 2.69 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2293 (Rfree = 0.000) for 3993 atoms. Found 129 (140 requested) and removed 71 (85 requested) atoms. Failed to save intermediate PDB Cycle 22: After refmac, R = 0.2197 (Rfree = 0.000) for 4044 atoms. Found 104 (138 requested) and removed 40 (86 requested) atoms. Cycle 23: After refmac, R = 0.2121 (Rfree = 0.000) for 4100 atoms. Found 98 (141 requested) and removed 38 (88 requested) atoms. Failed to save intermediate PDB Cycle 24: After refmac, R = 0.2095 (Rfree = 0.000) for 4154 atoms. Found 72 (142 requested) and removed 42 (89 requested) atoms. Cycle 25: After refmac, R = 0.2047 (Rfree = 0.000) for 4178 atoms. Found 80 (140 requested) and removed 41 (89 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 2.12 2.09 NCS extension: 12 residues added (14 deleted due to clashes), 4229 seeds are put forward Round 1: 418 peptides, 6 chains. Longest chain 107 peptides. Score 0.950 Round 2: 415 peptides, 7 chains. Longest chain 107 peptides. Score 0.946 Round 3: 405 peptides, 11 chains. Longest chain 107 peptides. Score 0.928 Round 4: 418 peptides, 7 chains. Longest chain 107 peptides. Score 0.947 Round 5: 413 peptides, 10 chains. Longest chain 107 peptides. Score 0.935 Taking the results from Round 1 Chains 6, Residues 412, Estimated correctness of the model 99.8 % 5 chains (409 residues) have been docked in sequence Building loops using Loopy2018 6 chains (412 residues) following loop building 5 chains (409 residues) in sequence following loop building ------------------------------------------------------ 42951 reflections ( 99.78 % complete ) and 3926 restraints for refining 4019 atoms. 501 conditional restraints added. Observations/parameters ratio is 2.67 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2255 (Rfree = 0.000) for 4019 atoms. Found 128 (131 requested) and removed 69 (86 requested) atoms. Failed to save intermediate PDB Cycle 27: After refmac, R = 0.2172 (Rfree = 0.000) for 4075 atoms. Found 79 (129 requested) and removed 24 (87 requested) atoms. Cycle 28: After refmac, R = 0.2116 (Rfree = 0.000) for 4123 atoms. Found 67 (130 requested) and removed 33 (88 requested) atoms. Failed to save intermediate PDB Cycle 29: After refmac, R = 0.2079 (Rfree = 0.000) for 4148 atoms. Found 78 (132 requested) and removed 32 (89 requested) atoms. Cycle 30: After refmac, R = 0.2057 (Rfree = 0.000) for 4190 atoms. Found 70 (132 requested) and removed 33 (89 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 2.12 2.09 NCS extension: 15 residues added (14 deleted due to clashes), 4243 seeds are put forward Round 1: 414 peptides, 7 chains. Longest chain 107 peptides. Score 0.945 Round 2: 414 peptides, 9 chains. Longest chain 107 peptides. Score 0.939 Round 3: 414 peptides, 8 chains. Longest chain 107 peptides. Score 0.942 Round 4: 410 peptides, 9 chains. Longest chain 107 peptides. Score 0.937 Round 5: 410 peptides, 12 chains. Longest chain 107 peptides. Score 0.927 Taking the results from Round 1 Chains 7, Residues 407, Estimated correctness of the model 99.7 % 6 chains (404 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 94 A and 98 A 6 chains (410 residues) following loop building 5 chains (407 residues) in sequence following loop building ------------------------------------------------------ Failed to save intermediate PDB 42951 reflections ( 99.78 % complete ) and 3973 restraints for refining 4021 atoms. 564 conditional restraints added. Observations/parameters ratio is 2.67 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2274 (Rfree = 0.000) for 4021 atoms. Found 123 (127 requested) and removed 75 (86 requested) atoms. Failed to save intermediate PDB Cycle 32: After refmac, R = 0.2191 (Rfree = 0.000) for 4065 atoms. Found 99 (125 requested) and removed 37 (87 requested) atoms. Cycle 33: After refmac, R = 0.2139 (Rfree = 0.000) for 4120 atoms. Found 88 (127 requested) and removed 37 (88 requested) atoms. Cycle 34: After refmac, R = 0.2099 (Rfree = 0.000) for 4163 atoms. Found 78 (128 requested) and removed 40 (89 requested) atoms. Cycle 35: After refmac, R = 0.2060 (Rfree = 0.000) for 4194 atoms. Found 74 (126 requested) and removed 36 (90 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 2.12 2.09 NCS extension: 27 residues added (27 deleted due to clashes), 4259 seeds are put forward Round 1: 413 peptides, 8 chains. Longest chain 107 peptides. Score 0.942 Round 2: 413 peptides, 8 chains. Longest chain 107 peptides. Score 0.942 Round 3: 411 peptides, 8 chains. Longest chain 107 peptides. Score 0.941 Round 4: 409 peptides, 11 chains. Longest chain 107 peptides. Score 0.930 Round 5: 412 peptides, 9 chains. Longest chain 107 peptides. Score 0.938 Taking the results from Round 2 Chains 8, Residues 405, Estimated correctness of the model 99.7 % 6 chains (397 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 90 A and 93 A 7 chains (407 residues) following loop building 5 chains (399 residues) in sequence following loop building ------------------------------------------------------ 42951 reflections ( 99.78 % complete ) and 4059 restraints for refining 4036 atoms. 697 conditional restraints added. Observations/parameters ratio is 2.66 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2266 (Rfree = 0.000) for 4036 atoms. Found 120 (120 requested) and removed 62 (86 requested) atoms. Cycle 37: After refmac, R = 0.2189 (Rfree = 0.000) for 4090 atoms. Found 87 (119 requested) and removed 30 (87 requested) atoms. Cycle 38: After refmac, R = 0.2133 (Rfree = 0.000) for 4138 atoms. Found 68 (120 requested) and removed 26 (88 requested) atoms. Failed to save intermediate PDB Cycle 39: After refmac, R = 0.2081 (Rfree = 0.000) for 4174 atoms. Found 65 (122 requested) and removed 31 (89 requested) atoms. Cycle 40: After refmac, R = 0.2061 (Rfree = 0.000) for 4194 atoms. Found 60 (122 requested) and removed 42 (90 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 2.12 2.09 NCS extension: 36 residues added (34 deleted due to clashes), 4249 seeds are put forward Round 1: 413 peptides, 7 chains. Longest chain 107 peptides. Score 0.945 Round 2: 409 peptides, 10 chains. Longest chain 107 peptides. Score 0.934 Round 3: 413 peptides, 7 chains. Longest chain 107 peptides. Score 0.945 Round 4: 410 peptides, 9 chains. Longest chain 107 peptides. Score 0.937 Round 5: 414 peptides, 7 chains. Longest chain 107 peptides. Score 0.945 Taking the results from Round 5 Chains 7, Residues 407, Estimated correctness of the model 99.7 % 5 chains (393 residues) have been docked in sequence Building loops using Loopy2018 7 chains (407 residues) following loop building 5 chains (393 residues) in sequence following loop building ------------------------------------------------------ Failed to save intermediate PDB 42951 reflections ( 99.78 % complete ) and 4104 restraints for refining 4029 atoms. 763 conditional restraints added. Observations/parameters ratio is 2.67 ------------------------------------------------------ Cycle 41: After refmac, R = 0.2292 (Rfree = 0.000) for 4029 atoms. Found 114 (114 requested) and removed 70 (86 requested) atoms. Cycle 42: After refmac, R = 0.2201 (Rfree = 0.000) for 4073 atoms. Found 76 (111 requested) and removed 26 (87 requested) atoms. Cycle 43: After refmac, R = 0.2131 (Rfree = 0.000) for 4120 atoms. Found 69 (113 requested) and removed 22 (88 requested) atoms. Cycle 44: After refmac, R = 0.2090 (Rfree = 0.000) for 4158 atoms. Found 72 (114 requested) and removed 34 (89 requested) atoms. Cycle 45: After refmac, R = 0.2067 (Rfree = 0.000) for 4193 atoms. Found 61 (115 requested) and removed 26 (89 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 2.13 2.10 NCS extension: 20 residues added (20 deleted due to clashes), 4248 seeds are put forward Round 1: 410 peptides, 6 chains. Longest chain 107 peptides. Score 0.947 Round 2: 412 peptides, 5 chains. Longest chain 107 peptides. Score 0.951 Round 3: 409 peptides, 7 chains. Longest chain 107 peptides. Score 0.943 Round 4: 403 peptides, 12 chains. Longest chain 90 peptides. Score 0.924 Round 5: 410 peptides, 7 chains. Longest chain 107 peptides. Score 0.944 Taking the results from Round 2 Last building cycle: Chain fragments will be rearranged Chains 5, Residues 407, Estimated correctness of the model 99.8 % 5 chains (407 residues) have been docked in sequence Sequence coverage is 99 % All DUM atoms will be removed Building loops using Loopy2018 5 chains (407 residues) following loop building 5 chains (407 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. Update only waters. 42951 reflections ( 99.78 % complete ) and 3403 restraints for refining 3331 atoms. Observations/parameters ratio is 3.22 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2842 (Rfree = 0.000) for 3331 atoms. Found 50 (91 requested) and removed 0 (91 requested) atoms. Cycle 47: After refmac, R = 0.2691 (Rfree = 0.000) for 3331 atoms. Found 29 (92 requested) and removed 0 (72 requested) atoms. Cycle 48: After refmac, R = 0.2573 (Rfree = 0.000) for 3331 atoms. Found 19 (93 requested) and removed 0 (73 requested) atoms. Failed to save intermediate PDB Cycle 49: After refmac, R = 0.2513 (Rfree = 0.000) for 3331 atoms. Found 13 (93 requested) and removed 2 (73 requested) atoms. Writing output files ... Failed to save intermediate PDB TimeTaking 76.92