Sun 23 Dec 22:22:49 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir Job ID is set to 2o1q-2.0-parrot-hancs X-ray data file /users/emra500/scratch/Dataset/hancsChltofom/2o1q-2.0-parrot-hancs.mtz Sequence file /users/emra500/scratch/Dataset/hancsChltofom/2o1q-2.0-parrot-hancs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2o1q-2.0-parrot-hancs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2o1q-2.0-parrot-hancs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2o1q-2.0-parrot-hancs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Sun 23 Dec 22:22:59 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2o1q-2.0-parrot-hancs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2o1q-2.0-parrot-hancs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 245 and 0 Target number of residues in the AU: 245 Target solvent content: 0.4812 Checking the provided sequence file Detected sequence length: 145 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 2 Adjusted target number of residues: 290 Adjusted target solvent content: 0.39 Input MTZ file: 2o1q-2.0-parrot-hancs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 92 Cell parameters: 61.883 61.883 133.475 90.000 90.000 90.000 Input sequence file: 2o1q-2.0-parrot-hancs.fasta_lf Building free atoms model in initial map for 2320 target number of atoms Had to go as low as 0.40 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 1 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 56.142 2.000 Wilson plot Bfac: 19.83 18257 reflections ( 99.95 % complete ) and 0 restraints for refining 2585 atoms. Observations/parameters ratio is 1.77 ------------------------------------------------------ Starting model: R = 0.3138 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.2562 (Rfree = 0.000) for 2585 atoms. Found 78 (88 requested) and removed 46 (44 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 1.95 2.26 NCS extension: 0 residues added, 2617 seeds are put forward Round 1: 231 peptides, 18 chains. Longest chain 30 peptides. Score 0.862 Round 2: 251 peptides, 13 chains. Longest chain 35 peptides. Score 0.920 Round 3: 258 peptides, 8 chains. Longest chain 74 peptides. Score 0.950 Round 4: 263 peptides, 8 chains. Longest chain 77 peptides. Score 0.953 Round 5: 258 peptides, 8 chains. Longest chain 77 peptides. Score 0.950 Taking the results from Round 4 Chains 8, Residues 255, Estimated correctness of the model 99.7 % 5 chains (240 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 24 B and 27 B 7 chains (257 residues) following loop building 4 chains (242 residues) in sequence following loop building ------------------------------------------------------ 18257 reflections ( 99.95 % complete ) and 2703 restraints for refining 2385 atoms. 688 conditional restraints added. Observations/parameters ratio is 1.91 ------------------------------------------------------ Cycle 1: After refmac, R = 0.2639 (Rfree = 0.000) for 2385 atoms. Found 69 (81 requested) and removed 43 (40 requested) atoms. Cycle 2: After refmac, R = 0.2249 (Rfree = 0.000) for 2399 atoms. Found 58 (80 requested) and removed 23 (41 requested) atoms. Cycle 3: After refmac, R = 0.2066 (Rfree = 0.000) for 2419 atoms. Found 58 (81 requested) and removed 18 (41 requested) atoms. Cycle 4: After refmac, R = 0.1934 (Rfree = 0.000) for 2451 atoms. Found 52 (82 requested) and removed 22 (42 requested) atoms. Cycle 5: After refmac, R = 0.1857 (Rfree = 0.000) for 2474 atoms. Found 52 (82 requested) and removed 27 (42 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 1.92 2.23 NCS extension: 64 residues added (9 deleted due to clashes), 2563 seeds are put forward Round 1: 264 peptides, 8 chains. Longest chain 77 peptides. Score 0.953 Round 2: 268 peptides, 7 chains. Longest chain 77 peptides. Score 0.959 Round 3: 273 peptides, 7 chains. Longest chain 88 peptides. Score 0.962 Round 4: 273 peptides, 6 chains. Longest chain 82 peptides. Score 0.965 Round 5: 273 peptides, 6 chains. Longest chain 82 peptides. Score 0.965 Taking the results from Round 5 Chains 6, Residues 267, Estimated correctness of the model 99.8 % 4 chains (259 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 83 A and 91 A 4 chains (271 residues) following loop building 3 chains (266 residues) in sequence following loop building ------------------------------------------------------ 18257 reflections ( 99.95 % complete ) and 2576 restraints for refining 2454 atoms. 425 conditional restraints added. Observations/parameters ratio is 1.86 ------------------------------------------------------ Cycle 6: After refmac, R = 0.1992 (Rfree = 0.000) for 2454 atoms. Found 71 (81 requested) and removed 43 (42 requested) atoms. Cycle 7: After refmac, R = 0.1862 (Rfree = 0.000) for 2479 atoms. Found 61 (79 requested) and removed 27 (42 requested) atoms. Cycle 8: After refmac, R = 0.1767 (Rfree = 0.000) for 2510 atoms. Found 53 (81 requested) and removed 27 (43 requested) atoms. Failed to save intermediate PDB Cycle 9: After refmac, R = 0.1717 (Rfree = 0.000) for 2534 atoms. Found 51 (79 requested) and removed 36 (43 requested) atoms. Cycle 10: After refmac, R = 0.1702 (Rfree = 0.000) for 2546 atoms. Found 55 (78 requested) and removed 38 (43 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 1.92 2.23 NCS extension: 14 residues added (142 deleted due to clashes), 2577 seeds are put forward Round 1: 275 peptides, 6 chains. Longest chain 87 peptides. Score 0.966 Round 2: 277 peptides, 6 chains. Longest chain 88 peptides. Score 0.967 Round 3: 278 peptides, 6 chains. Longest chain 88 peptides. Score 0.967 Round 4: 279 peptides, 5 chains. Longest chain 88 peptides. Score 0.971 Round 5: 279 peptides, 6 chains. Longest chain 81 peptides. Score 0.968 Taking the results from Round 4 Chains 6, Residues 274, Estimated correctness of the model 99.9 % 4 chains (261 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 87 A and 94 A 4 chains (277 residues) following loop building 3 chains (267 residues) in sequence following loop building ------------------------------------------------------ 18257 reflections ( 99.95 % complete ) and 2602 restraints for refining 2480 atoms. 427 conditional restraints added. Observations/parameters ratio is 1.84 ------------------------------------------------------ Cycle 11: After refmac, R = 0.1872 (Rfree = 0.000) for 2480 atoms. Found 74 (74 requested) and removed 49 (42 requested) atoms. Cycle 12: After refmac, R = 0.1760 (Rfree = 0.000) for 2502 atoms. Found 72 (73 requested) and removed 27 (42 requested) atoms. Cycle 13: After refmac, R = 0.1672 (Rfree = 0.000) for 2547 atoms. Found 40 (75 requested) and removed 27 (43 requested) atoms. Cycle 14: After refmac, R = 0.1629 (Rfree = 0.000) for 2555 atoms. Found 52 (73 requested) and removed 17 (43 requested) atoms. Cycle 15: After refmac, R = 0.1591 (Rfree = 0.000) for 2586 atoms. Found 34 (74 requested) and removed 44 (44 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 1.93 2.24 NCS extension: 7 residues added (147 deleted due to clashes), 2583 seeds are put forward Round 1: 276 peptides, 6 chains. Longest chain 87 peptides. Score 0.967 Round 2: 278 peptides, 6 chains. Longest chain 88 peptides. Score 0.967 Round 3: 279 peptides, 5 chains. Longest chain 88 peptides. Score 0.971 Round 4: 280 peptides, 5 chains. Longest chain 88 peptides. Score 0.971 Round 5: 278 peptides, 7 chains. Longest chain 81 peptides. Score 0.964 Taking the results from Round 4 Chains 7, Residues 275, Estimated correctness of the model 99.9 % 5 chains (269 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 76 A and 81 A Built loop between residues 88 A and 91 A Built loop between residues 87 B and 94 B 2 chains (281 residues) following loop building 2 chains (281 residues) in sequence following loop building ------------------------------------------------------ 18257 reflections ( 99.95 % complete ) and 2502 restraints for refining 2486 atoms. 276 conditional restraints added. Observations/parameters ratio is 1.84 ------------------------------------------------------ Cycle 16: After refmac, R = 0.1829 (Rfree = 0.000) for 2486 atoms. Found 70 (70 requested) and removed 43 (42 requested) atoms. Cycle 17: After refmac, R = 0.1680 (Rfree = 0.000) for 2512 atoms. Found 59 (69 requested) and removed 12 (43 requested) atoms. Cycle 18: After refmac, R = 0.1589 (Rfree = 0.000) for 2556 atoms. Found 46 (70 requested) and removed 32 (43 requested) atoms. Cycle 19: After refmac, R = 0.1559 (Rfree = 0.000) for 2568 atoms. Found 51 (68 requested) and removed 26 (43 requested) atoms. Cycle 20: After refmac, R = 0.1544 (Rfree = 0.000) for 2592 atoms. Found 45 (67 requested) and removed 47 (44 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 1.93 2.24 NCS extension: 0 residues added, 2590 seeds are put forward Round 1: 278 peptides, 5 chains. Longest chain 88 peptides. Score 0.971 Round 2: 279 peptides, 5 chains. Longest chain 89 peptides. Score 0.971 Round 3: 279 peptides, 5 chains. Longest chain 89 peptides. Score 0.971 Round 4: 280 peptides, 4 chains. Longest chain 89 peptides. Score 0.975 Round 5: 278 peptides, 6 chains. Longest chain 89 peptides. Score 0.967 Taking the results from Round 4 Chains 4, Residues 276, Estimated correctness of the model 99.9 % 4 chains (276 residues) have been docked in sequence Building loops using Loopy2018 4 chains (276 residues) following loop building 4 chains (276 residues) in sequence following loop building ------------------------------------------------------ 18257 reflections ( 99.95 % complete ) and 2602 restraints for refining 2500 atoms. 420 conditional restraints added. Observations/parameters ratio is 1.83 ------------------------------------------------------ Cycle 21: After refmac, R = 0.1750 (Rfree = 0.000) for 2500 atoms. Found 63 (63 requested) and removed 43 (42 requested) atoms. Cycle 22: After refmac, R = 0.1664 (Rfree = 0.000) for 2518 atoms. Found 59 (62 requested) and removed 13 (43 requested) atoms. Cycle 23: After refmac, R = 0.1588 (Rfree = 0.000) for 2564 atoms. Found 47 (63 requested) and removed 22 (43 requested) atoms. Failed to save intermediate PDB Cycle 24: After refmac, R = 0.1548 (Rfree = 0.000) for 2587 atoms. Found 42 (63 requested) and removed 33 (44 requested) atoms. Cycle 25: After refmac, R = 0.1543 (Rfree = 0.000) for 2593 atoms. Found 48 (62 requested) and removed 41 (44 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 1.92 2.23 NCS extension: 0 residues added (7 deleted due to clashes), 2600 seeds are put forward Round 1: 280 peptides, 5 chains. Longest chain 89 peptides. Score 0.971 Round 2: 279 peptides, 5 chains. Longest chain 89 peptides. Score 0.971 Round 3: 279 peptides, 5 chains. Longest chain 89 peptides. Score 0.971 Round 4: 280 peptides, 4 chains. Longest chain 89 peptides. Score 0.975 Round 5: 279 peptides, 5 chains. Longest chain 89 peptides. Score 0.971 Taking the results from Round 4 Chains 4, Residues 276, Estimated correctness of the model 99.9 % 4 chains (276 residues) have been docked in sequence Building loops using Loopy2018 4 chains (276 residues) following loop building 4 chains (276 residues) in sequence following loop building ------------------------------------------------------ 18257 reflections ( 99.95 % complete ) and 2602 restraints for refining 2497 atoms. 420 conditional restraints added. Observations/parameters ratio is 1.83 ------------------------------------------------------ Cycle 26: After refmac, R = 0.1747 (Rfree = 0.000) for 2497 atoms. Found 58 (58 requested) and removed 42 (42 requested) atoms. Cycle 27: After refmac, R = 0.1644 (Rfree = 0.000) for 2508 atoms. Found 57 (57 requested) and removed 20 (43 requested) atoms. Cycle 28: After refmac, R = 0.1594 (Rfree = 0.000) for 2544 atoms. Found 51 (57 requested) and removed 32 (43 requested) atoms. Cycle 29: After refmac, R = 0.1591 (Rfree = 0.000) for 2560 atoms. Found 46 (56 requested) and removed 43 (43 requested) atoms. Cycle 30: After refmac, R = 0.1562 (Rfree = 0.000) for 2561 atoms. Found 54 (54 requested) and removed 36 (43 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 1.92 2.23 NCS extension: 1 residues added (6 deleted due to clashes), 2580 seeds are put forward Round 1: 279 peptides, 6 chains. Longest chain 89 peptides. Score 0.968 Round 2: 278 peptides, 5 chains. Longest chain 89 peptides. Score 0.971 Round 3: 277 peptides, 7 chains. Longest chain 89 peptides. Score 0.963 Round 4: 279 peptides, 5 chains. Longest chain 89 peptides. Score 0.971 Round 5: 280 peptides, 4 chains. Longest chain 89 peptides. Score 0.975 Taking the results from Round 5 Chains 4, Residues 276, Estimated correctness of the model 99.9 % 4 chains (276 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 90 A and 94 A Built loop between residues 90 B and 94 B 2 chains (282 residues) following loop building 2 chains (282 residues) in sequence following loop building ------------------------------------------------------ 18257 reflections ( 99.95 % complete ) and 2531 restraints for refining 2507 atoms. 297 conditional restraints added. Observations/parameters ratio is 1.82 ------------------------------------------------------ Cycle 31: After refmac, R = 0.1782 (Rfree = 0.000) for 2507 atoms. Found 51 (51 requested) and removed 44 (42 requested) atoms. Cycle 32: After refmac, R = 0.1657 (Rfree = 0.000) for 2511 atoms. Found 50 (50 requested) and removed 19 (43 requested) atoms. Cycle 33: After refmac, R = 0.1608 (Rfree = 0.000) for 2541 atoms. Found 50 (50 requested) and removed 20 (43 requested) atoms. Cycle 34: After refmac, R = 0.1574 (Rfree = 0.000) for 2567 atoms. Found 42 (50 requested) and removed 27 (43 requested) atoms. Cycle 35: After refmac, R = 0.1560 (Rfree = 0.000) for 2580 atoms. Found 36 (49 requested) and removed 32 (44 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 1.94 2.25 NCS extension: 4 residues added (138 deleted due to clashes), 2590 seeds are put forward Round 1: 277 peptides, 6 chains. Longest chain 88 peptides. Score 0.967 Round 2: 278 peptides, 5 chains. Longest chain 89 peptides. Score 0.971 Round 3: 278 peptides, 6 chains. Longest chain 89 peptides. Score 0.967 Round 4: 278 peptides, 6 chains. Longest chain 89 peptides. Score 0.967 Round 5: 277 peptides, 6 chains. Longest chain 89 peptides. Score 0.967 Taking the results from Round 2 Chains 6, Residues 273, Estimated correctness of the model 99.9 % 5 chains (270 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 87 A and 94 A Built loop between residues 106 A and 109 A Built loop between residues 90 B and 94 B 2 chains (281 residues) following loop building 2 chains (281 residues) in sequence following loop building ------------------------------------------------------ Failed to save intermediate PDB 18257 reflections ( 99.95 % complete ) and 2516 restraints for refining 2497 atoms. 290 conditional restraints added. Observations/parameters ratio is 1.83 ------------------------------------------------------ Cycle 36: After refmac, R = 0.1773 (Rfree = 0.000) for 2497 atoms. Found 46 (46 requested) and removed 48 (42 requested) atoms. Cycle 37: After refmac, R = 0.1685 (Rfree = 0.000) for 2491 atoms. Found 44 (44 requested) and removed 8 (42 requested) atoms. Cycle 38: After refmac, R = 0.1616 (Rfree = 0.000) for 2526 atoms. Found 45 (45 requested) and removed 18 (43 requested) atoms. Cycle 39: After refmac, R = 0.1584 (Rfree = 0.000) for 2551 atoms. Found 44 (45 requested) and removed 27 (43 requested) atoms. Cycle 40: After refmac, R = 0.1572 (Rfree = 0.000) for 2566 atoms. Found 42 (43 requested) and removed 30 (43 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 1.93 2.24 NCS extension: 3 residues added (138 deleted due to clashes), 2583 seeds are put forward Round 1: 277 peptides, 6 chains. Longest chain 89 peptides. Score 0.967 Round 2: 277 peptides, 5 chains. Longest chain 89 peptides. Score 0.970 Round 3: 277 peptides, 5 chains. Longest chain 89 peptides. Score 0.970 Round 4: 277 peptides, 5 chains. Longest chain 89 peptides. Score 0.970 Round 5: 275 peptides, 6 chains. Longest chain 89 peptides. Score 0.966 Taking the results from Round 4 Chains 5, Residues 272, Estimated correctness of the model 99.9 % 5 chains (272 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 89 A and 94 A Built loop between residues 106 A and 109 A Built loop between residues 90 B and 94 B 2 chains (281 residues) following loop building 2 chains (281 residues) in sequence following loop building ------------------------------------------------------ 18257 reflections ( 99.95 % complete ) and 2514 restraints for refining 2492 atoms. 288 conditional restraints added. Observations/parameters ratio is 1.83 ------------------------------------------------------ Cycle 41: After refmac, R = 0.1735 (Rfree = 0.000) for 2492 atoms. Found 42 (42 requested) and removed 36 (42 requested) atoms. Cycle 42: After refmac, R = 0.1649 (Rfree = 0.000) for 2496 atoms. Found 42 (42 requested) and removed 10 (42 requested) atoms. Cycle 43: After refmac, R = 0.1597 (Rfree = 0.000) for 2526 atoms. Found 39 (43 requested) and removed 15 (43 requested) atoms. Cycle 44: After refmac, R = 0.1557 (Rfree = 0.000) for 2547 atoms. Found 43 (43 requested) and removed 22 (43 requested) atoms. Cycle 45: After refmac, R = 0.1542 (Rfree = 0.000) for 2568 atoms. Found 40 (43 requested) and removed 30 (43 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 1.93 2.24 NCS extension: 4 residues added (137 deleted due to clashes), 2584 seeds are put forward Round 1: 275 peptides, 8 chains. Longest chain 87 peptides. Score 0.959 Round 2: 277 peptides, 6 chains. Longest chain 88 peptides. Score 0.967 Round 3: 277 peptides, 6 chains. Longest chain 88 peptides. Score 0.967 Round 4: 278 peptides, 6 chains. Longest chain 77 peptides. Score 0.967 Round 5: 279 peptides, 5 chains. Longest chain 89 peptides. Score 0.971 Taking the results from Round 5 Last building cycle: Chain fragments will be rearranged Chains 5, Residues 274, Estimated correctness of the model 99.9 % 5 chains (274 residues) have been docked in sequence Sequence coverage is 99 % All DUM atoms will be removed Building loops using Loopy2018 Built loop between residues 78 A and 81 A Built loop between residues 90 A and 94 A Built loop between residues 90 B and 94 B 2 chains (282 residues) following loop building 2 chains (282 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. Update only waters. 18257 reflections ( 99.95 % complete ) and 2234 restraints for refining 2160 atoms. Observations/parameters ratio is 2.11 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2558 (Rfree = 0.000) for 2160 atoms. Found 36 (36 requested) and removed 0 (36 requested) atoms. Cycle 47: After refmac, R = 0.2356 (Rfree = 0.000) for 2160 atoms. Found 29 (37 requested) and removed 0 (37 requested) atoms. Cycle 48: After refmac, R = 0.2196 (Rfree = 0.000) for 2160 atoms. Found 20 (38 requested) and removed 8 (38 requested) atoms. Cycle 49: After refmac, R = 0.2213 (Rfree = 0.000) for 2160 atoms. TimeTaking 52.75