Sun 23 Dec 22:22:43 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir Job ID is set to 2f4p-1.9-parrot-hancs X-ray data file /users/emra500/scratch/Dataset/hancsChltofom/2f4p-1.9-parrot-hancs.mtz Sequence file /users/emra500/scratch/Dataset/hancsChltofom/2f4p-1.9-parrot-hancs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2f4p-1.9-parrot-hancs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2f4p-1.9-parrot-hancs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2f4p-1.9-parrot-hancs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Sun 23 Dec 22:22:52 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2f4p-1.9-parrot-hancs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2f4p-1.9-parrot-hancs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 529 and 0 Target number of residues in the AU: 529 Target solvent content: 0.4892 Checking the provided sequence file Detected sequence length: 147 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 4 Adjusted target number of residues: 588 Adjusted target solvent content: 0.43 Input MTZ file: 2f4p-1.9-parrot-hancs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 170 Cell parameters: 71.746 71.746 188.600 90.000 90.000 120.000 Input sequence file: 2f4p-1.9-parrot-hancs.fasta_lf Building free atoms model in initial map for 4704 target number of atoms Had to go as low as 0.40 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 1 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 21.543 1.900 Wilson plot Bfac: 21.23 43044 reflections ( 99.90 % complete ) and 0 restraints for refining 5189 atoms. Observations/parameters ratio is 2.07 ------------------------------------------------------ Starting model: R = 0.3363 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.2851 (Rfree = 0.000) for 5189 atoms. Found 193 (205 requested) and removed 108 (102 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 1.95 2.14 NCS extension: 0 residues added, 5274 seeds are put forward Round 1: 425 peptides, 31 chains. Longest chain 58 peptides. Score 0.831 Round 2: 454 peptides, 21 chains. Longest chain 60 peptides. Score 0.896 Round 3: 487 peptides, 15 chains. Longest chain 107 peptides. Score 0.931 Round 4: 498 peptides, 9 chains. Longest chain 100 peptides. Score 0.951 Round 5: 499 peptides, 10 chains. Longest chain 128 peptides. Score 0.948 Taking the results from Round 4 Chains 14, Residues 489, Estimated correctness of the model 99.8 % 9 chains (479 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 41 A and 46 A Built loop between residues 41 C and 48 C Built loop between residues 42 B and 50 B Built loop between residues 62 D and 65 D Built loop between residues 107 D and 110 D 8 chains (506 residues) following loop building 4 chains (500 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4749 restraints for refining 4736 atoms. 656 conditional restraints added. Observations/parameters ratio is 2.27 ------------------------------------------------------ Cycle 1: After refmac, R = 0.3099 (Rfree = 0.000) for 4736 atoms. Found 162 (187 requested) and removed 107 (93 requested) atoms. Cycle 2: After refmac, R = 0.2876 (Rfree = 0.000) for 4780 atoms. Found 102 (185 requested) and removed 93 (94 requested) atoms. Cycle 3: After refmac, R = 0.2594 (Rfree = 0.000) for 4770 atoms. Found 113 (181 requested) and removed 54 (94 requested) atoms. Cycle 4: After refmac, R = 0.2441 (Rfree = 0.000) for 4791 atoms. Found 105 (183 requested) and removed 33 (95 requested) atoms. Cycle 5: After refmac, R = 0.2338 (Rfree = 0.000) for 4833 atoms. Found 118 (184 requested) and removed 30 (96 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 1.93 2.12 NCS extension: 4 residues added (70 deleted due to clashes), 4935 seeds are put forward Round 1: 501 peptides, 8 chains. Longest chain 129 peptides. Score 0.954 Round 2: 504 peptides, 9 chains. Longest chain 100 peptides. Score 0.952 Round 3: 501 peptides, 10 chains. Longest chain 129 peptides. Score 0.949 Round 4: 506 peptides, 9 chains. Longest chain 116 peptides. Score 0.953 Round 5: 505 peptides, 9 chains. Longest chain 124 peptides. Score 0.953 Taking the results from Round 1 Chains 11, Residues 493, Estimated correctness of the model 99.8 % 8 chains (488 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 41 A and 46 A Built loop between residues 42 D and 49 D Built loop between residues 67 D and 71 D 7 chains (505 residues) following loop building 5 chains (501 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4805 restraints for refining 4801 atoms. 716 conditional restraints added. Observations/parameters ratio is 2.24 ------------------------------------------------------ Cycle 6: After refmac, R = 0.2382 (Rfree = 0.000) for 4801 atoms. Found 137 (182 requested) and removed 101 (95 requested) atoms. Cycle 7: After refmac, R = 0.2229 (Rfree = 0.000) for 4828 atoms. Found 123 (180 requested) and removed 48 (95 requested) atoms. Cycle 8: After refmac, R = 0.2123 (Rfree = 0.000) for 4895 atoms. Found 113 (182 requested) and removed 28 (97 requested) atoms. Cycle 9: After refmac, R = 0.2054 (Rfree = 0.000) for 4967 atoms. Found 98 (185 requested) and removed 33 (98 requested) atoms. Cycle 10: After refmac, R = 0.2005 (Rfree = 0.000) for 5021 atoms. Found 96 (187 requested) and removed 41 (99 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 1.92 2.11 NCS extension: 10 residues added (52 deleted due to clashes), 5091 seeds are put forward Round 1: 510 peptides, 6 chains. Longest chain 129 peptides. Score 0.961 Round 2: 507 peptides, 8 chains. Longest chain 128 peptides. Score 0.956 Round 3: 507 peptides, 10 chains. Longest chain 100 peptides. Score 0.951 Round 4: 506 peptides, 10 chains. Longest chain 100 peptides. Score 0.951 Round 5: 502 peptides, 10 chains. Longest chain 128 peptides. Score 0.949 Taking the results from Round 1 Chains 8, Residues 504, Estimated correctness of the model 99.8 % 6 chains (502 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 42 A and 46 A Built loop between residues 41 C and 46 C 5 chains (510 residues) following loop building 5 chains (510 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4708 restraints for refining 4897 atoms. 559 conditional restraints added. Observations/parameters ratio is 2.20 ------------------------------------------------------ Cycle 11: After refmac, R = 0.2163 (Rfree = 0.000) for 4897 atoms. Found 142 (182 requested) and removed 101 (97 requested) atoms. Cycle 12: After refmac, R = 0.2072 (Rfree = 0.000) for 4934 atoms. Found 104 (179 requested) and removed 34 (97 requested) atoms. Cycle 13: After refmac, R = 0.2007 (Rfree = 0.000) for 4994 atoms. Found 96 (182 requested) and removed 20 (99 requested) atoms. Cycle 14: After refmac, R = 0.1960 (Rfree = 0.000) for 5065 atoms. Found 80 (185 requested) and removed 33 (100 requested) atoms. Cycle 15: After refmac, R = 0.1935 (Rfree = 0.000) for 5104 atoms. Found 59 (186 requested) and removed 37 (101 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 1.92 2.11 NCS extension: 0 residues added, 5126 seeds are put forward Round 1: 510 peptides, 6 chains. Longest chain 128 peptides. Score 0.961 Round 2: 510 peptides, 7 chains. Longest chain 128 peptides. Score 0.959 Round 3: 511 peptides, 8 chains. Longest chain 100 peptides. Score 0.957 Round 4: 511 peptides, 6 chains. Longest chain 129 peptides. Score 0.961 Round 5: 509 peptides, 9 chains. Longest chain 129 peptides. Score 0.954 Taking the results from Round 4 Chains 6, Residues 505, Estimated correctness of the model 99.8 % 6 chains (505 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 75 A and 78 A Built loop between residues 42 C and 46 C 4 chains (510 residues) following loop building 4 chains (510 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4747 restraints for refining 4918 atoms. 593 conditional restraints added. Observations/parameters ratio is 2.19 ------------------------------------------------------ Cycle 16: After refmac, R = 0.2111 (Rfree = 0.000) for 4918 atoms. Found 174 (175 requested) and removed 88 (97 requested) atoms. Cycle 17: After refmac, R = 0.2039 (Rfree = 0.000) for 4995 atoms. Found 105 (174 requested) and removed 39 (99 requested) atoms. Cycle 18: After refmac, R = 0.1976 (Rfree = 0.000) for 5049 atoms. Found 83 (176 requested) and removed 18 (100 requested) atoms. Cycle 19: After refmac, R = 0.1930 (Rfree = 0.000) for 5107 atoms. Found 67 (178 requested) and removed 28 (101 requested) atoms. Cycle 20: After refmac, R = 0.1896 (Rfree = 0.000) for 5131 atoms. Found 91 (180 requested) and removed 26 (102 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 1.92 2.11 NCS extension: 0 residues added, 5197 seeds are put forward Round 1: 514 peptides, 5 chains. Longest chain 130 peptides. Score 0.964 Round 2: 510 peptides, 7 chains. Longest chain 128 peptides. Score 0.959 Round 3: 510 peptides, 8 chains. Longest chain 128 peptides. Score 0.956 Round 4: 511 peptides, 9 chains. Longest chain 130 peptides. Score 0.954 Round 5: 509 peptides, 9 chains. Longest chain 128 peptides. Score 0.954 Taking the results from Round 1 Chains 6, Residues 509, Estimated correctness of the model 99.9 % 5 chains (506 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 42 D and 46 D 5 chains (512 residues) following loop building 4 chains (509 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4744 restraints for refining 4952 atoms. 586 conditional restraints added. Observations/parameters ratio is 2.17 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2064 (Rfree = 0.000) for 4952 atoms. Found 168 (172 requested) and removed 84 (98 requested) atoms. Cycle 22: After refmac, R = 0.1984 (Rfree = 0.000) for 5025 atoms. Found 92 (175 requested) and removed 35 (99 requested) atoms. Cycle 23: After refmac, R = 0.1933 (Rfree = 0.000) for 5075 atoms. Found 88 (177 requested) and removed 27 (100 requested) atoms. Cycle 24: After refmac, R = 0.1895 (Rfree = 0.000) for 5132 atoms. Found 79 (179 requested) and removed 44 (101 requested) atoms. Cycle 25: After refmac, R = 0.1878 (Rfree = 0.000) for 5162 atoms. Found 79 (175 requested) and removed 46 (102 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 1.91 2.10 NCS extension: 0 residues added, 5196 seeds are put forward Round 1: 515 peptides, 5 chains. Longest chain 131 peptides. Score 0.964 Round 2: 515 peptides, 6 chains. Longest chain 131 peptides. Score 0.962 Round 3: 510 peptides, 11 chains. Longest chain 100 peptides. Score 0.949 Round 4: 512 peptides, 8 chains. Longest chain 130 peptides. Score 0.957 Round 5: 513 peptides, 10 chains. Longest chain 100 peptides. Score 0.953 Taking the results from Round 1 Chains 5, Residues 510, Estimated correctness of the model 99.9 % 5 chains (510 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 42 D and 46 D 4 chains (513 residues) following loop building 4 chains (513 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4803 restraints for refining 5001 atoms. 626 conditional restraints added. Observations/parameters ratio is 2.15 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2027 (Rfree = 0.000) for 5001 atoms. Found 164 (166 requested) and removed 85 (99 requested) atoms. Cycle 27: After refmac, R = 0.1967 (Rfree = 0.000) for 5066 atoms. Found 116 (165 requested) and removed 37 (100 requested) atoms. Cycle 28: After refmac, R = 0.1916 (Rfree = 0.000) for 5138 atoms. Found 92 (167 requested) and removed 54 (102 requested) atoms. Cycle 29: After refmac, R = 0.1875 (Rfree = 0.000) for 5173 atoms. Found 94 (164 requested) and removed 42 (102 requested) atoms. Cycle 30: After refmac, R = 0.1856 (Rfree = 0.000) for 5222 atoms. Found 77 (166 requested) and removed 59 (103 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 1.91 2.10 NCS extension: 0 residues added, 5240 seeds are put forward Round 1: 512 peptides, 5 chains. Longest chain 129 peptides. Score 0.964 Round 2: 512 peptides, 8 chains. Longest chain 131 peptides. Score 0.957 Round 3: 508 peptides, 9 chains. Longest chain 91 peptides. Score 0.954 Round 4: 508 peptides, 9 chains. Longest chain 103 peptides. Score 0.954 Round 5: 510 peptides, 8 chains. Longest chain 100 peptides. Score 0.956 Taking the results from Round 1 Chains 5, Residues 507, Estimated correctness of the model 99.9 % 5 chains (507 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 42 A and 46 A 4 chains (510 residues) following loop building 4 chains (510 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4796 restraints for refining 5013 atoms. 642 conditional restraints added. Observations/parameters ratio is 2.15 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2002 (Rfree = 0.000) for 5013 atoms. Found 154 (154 requested) and removed 83 (99 requested) atoms. Cycle 32: After refmac, R = 0.1949 (Rfree = 0.000) for 5076 atoms. Found 102 (153 requested) and removed 36 (100 requested) atoms. Cycle 33: After refmac, R = 0.1893 (Rfree = 0.000) for 5139 atoms. Found 83 (154 requested) and removed 35 (101 requested) atoms. Cycle 34: After refmac, R = 0.1858 (Rfree = 0.000) for 5181 atoms. Found 70 (156 requested) and removed 39 (102 requested) atoms. Cycle 35: After refmac, R = 0.1837 (Rfree = 0.000) for 5206 atoms. Found 75 (152 requested) and removed 47 (103 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 1.91 2.10 NCS extension: 0 residues added, 5235 seeds are put forward Round 1: 512 peptides, 5 chains. Longest chain 128 peptides. Score 0.964 Round 2: 511 peptides, 7 chains. Longest chain 127 peptides. Score 0.959 Round 3: 509 peptides, 9 chains. Longest chain 100 peptides. Score 0.954 Round 4: 510 peptides, 8 chains. Longest chain 100 peptides. Score 0.956 Round 5: 508 peptides, 9 chains. Longest chain 129 peptides. Score 0.954 Taking the results from Round 1 Chains 6, Residues 507, Estimated correctness of the model 99.9 % 5 chains (504 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 42 D and 46 D 5 chains (510 residues) following loop building 4 chains (507 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4800 restraints for refining 5011 atoms. 657 conditional restraints added. Observations/parameters ratio is 2.15 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2005 (Rfree = 0.000) for 5011 atoms. Found 143 (143 requested) and removed 73 (99 requested) atoms. Cycle 37: After refmac, R = 0.1943 (Rfree = 0.000) for 5076 atoms. Found 110 (141 requested) and removed 30 (100 requested) atoms. Cycle 38: After refmac, R = 0.1893 (Rfree = 0.000) for 5149 atoms. Found 73 (143 requested) and removed 24 (102 requested) atoms. Cycle 39: After refmac, R = 0.1859 (Rfree = 0.000) for 5192 atoms. Found 72 (144 requested) and removed 36 (103 requested) atoms. Cycle 40: After refmac, R = 0.1836 (Rfree = 0.000) for 5223 atoms. Found 77 (145 requested) and removed 33 (103 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 1.91 2.10 NCS extension: 0 residues added (3 deleted due to clashes), 5268 seeds are put forward Round 1: 507 peptides, 8 chains. Longest chain 116 peptides. Score 0.956 Round 2: 510 peptides, 10 chains. Longest chain 100 peptides. Score 0.952 Round 3: 510 peptides, 11 chains. Longest chain 100 peptides. Score 0.949 Round 4: 510 peptides, 9 chains. Longest chain 128 peptides. Score 0.954 Round 5: 507 peptides, 12 chains. Longest chain 67 peptides. Score 0.946 Taking the results from Round 1 Chains 9, Residues 499, Estimated correctness of the model 99.8 % 7 chains (489 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 42 A and 46 A Built loop between residues 42 C and 46 C Built loop between residues 68 B and 71 B 6 chains (507 residues) following loop building 4 chains (497 residues) in sequence following loop building ------------------------------------------------------ 43044 reflections ( 99.90 % complete ) and 4928 restraints for refining 5008 atoms. 845 conditional restraints added. Observations/parameters ratio is 2.15 ------------------------------------------------------ Cycle 41: After refmac, R = 0.2024 (Rfree = 0.000) for 5008 atoms. Found 139 (139 requested) and removed 67 (99 requested) atoms. Cycle 42: After refmac, R = 0.1950 (Rfree = 0.000) for 5075 atoms. Found 91 (141 requested) and removed 29 (100 requested) atoms. Cycle 43: After refmac, R = 0.1906 (Rfree = 0.000) for 5134 atoms. Found 87 (142 requested) and removed 30 (101 requested) atoms. Cycle 44: After refmac, R = 0.1878 (Rfree = 0.000) for 5187 atoms. Found 80 (143 requested) and removed 42 (102 requested) atoms. Cycle 45: After refmac, R = 0.1855 (Rfree = 0.000) for 5220 atoms. Found 74 (141 requested) and removed 44 (103 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 1.91 2.10 NCS extension: 0 residues added (23 deleted due to clashes), 5250 seeds are put forward Round 1: 509 peptides, 7 chains. Longest chain 126 peptides. Score 0.958 Round 2: 507 peptides, 9 chains. Longest chain 100 peptides. Score 0.953 Round 3: 507 peptides, 10 chains. Longest chain 127 peptides. Score 0.951 Round 4: 510 peptides, 8 chains. Longest chain 127 peptides. Score 0.956 Round 5: 507 peptides, 10 chains. Longest chain 100 peptides. Score 0.951 Taking the results from Round 1 Last building cycle: Chain fragments will be rearranged Chains 7, Residues 502, Estimated correctness of the model 99.8 % 6 chains (492 residues) have been docked in sequence Sequence coverage is 97 % All DUM atoms will be removed Building loops using Loopy2018 Built loop between residues 42 C and 46 C Built loop between residues 42 D and 46 D 5 chains (508 residues) following loop building 4 chains (498 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. Update only waters. Failed to save intermediate PDB 43044 reflections ( 99.90 % complete ) and 4092 restraints for refining 3975 atoms. Observations/parameters ratio is 2.71 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2731 (Rfree = 0.000) for 3975 atoms. Found 103 (104 requested) and removed 0 (104 requested) atoms. Cycle 47: After refmac, R = 0.2544 (Rfree = 0.000) for 3975 atoms. Found 56 (106 requested) and removed 0 (80 requested) atoms. Cycle 48: After refmac, R = 0.2400 (Rfree = 0.000) for 3975 atoms. Found 34 (108 requested) and removed 0 (81 requested) atoms. Cycle 49: After refmac, R = 0.2332 (Rfree = 0.000) for 3975 atoms. TimeTaking 81.48