null Sun 23 Dec 22:22:59 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir Job ID is set to 2o8q-2.5-parrot-hancs X-ray data file /users/emra500/scratch/Dataset/hancsChltofom/2o8q-2.5-parrot-hancs.mtz Sequence file /users/emra500/scratch/Dataset/hancsChltofom/2o8q-2.5-parrot-hancs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2o8q-2.5-parrot-hancs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2o8q-2.5-parrot-hancs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2o8q-2.5-parrot-hancs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Sun 23 Dec 22:23:07 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2o8q-2.5-parrot-hancs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/2o8q-2.5-parrot-hancs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 347 and 0 Target number of residues in the AU: 347 Target solvent content: 0.5275 Checking the provided sequence file Detected sequence length: 134 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 2 Adjusted target number of residues: 268 Adjusted target solvent content: 0.64 Input MTZ file: 2o8q-2.5-parrot-hancs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 94 Cell parameters: 103.170 103.170 74.680 90.000 90.000 90.000 Input sequence file: 2o8q-2.5-parrot-hancs.fasta_lf Building free atoms model in initial map for 2144 target number of atoms Had to go as low as 0.75 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 3 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 29.026 2.501 Wilson plot Bfac: 29.54 14305 reflections ( 98.97 % complete ) and 0 restraints for refining 2375 atoms. Observations/parameters ratio is 1.51 ------------------------------------------------------ Starting model: R = 0.3018 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.2262 (Rfree = 0.000) for 2375 atoms. Found 39 (43 requested) and removed 27 (21 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 2.45 2.02 NCS extension: 0 residues added, 2387 seeds are put forward Round 1: 195 peptides, 22 chains. Longest chain 34 peptides. Score 0.606 Round 2: 208 peptides, 19 chains. Longest chain 29 peptides. Score 0.681 Round 3: 213 peptides, 15 chains. Longest chain 35 peptides. Score 0.739 Round 4: 225 peptides, 10 chains. Longest chain 78 peptides. Score 0.811 Round 5: 228 peptides, 7 chains. Longest chain 101 peptides. Score 0.842 Taking the results from Round 5 Chains 7, Residues 221, Estimated correctness of the model 95.1 % 4 chains (197 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 49 A and 57 A Built loop between residues 116 A and 119 A 5 chains (230 residues) following loop building 2 chains (206 residues) in sequence following loop building ------------------------------------------------------ 14305 reflections ( 98.97 % complete ) and 2463 restraints for refining 2199 atoms. 707 conditional restraints added. Observations/parameters ratio is 1.63 ------------------------------------------------------ Cycle 1: After refmac, R = 0.2432 (Rfree = 0.000) for 2199 atoms. Found 39 (39 requested) and removed 29 (19 requested) atoms. Cycle 2: After refmac, R = 0.2162 (Rfree = 0.000) for 2176 atoms. Found 39 (39 requested) and removed 24 (20 requested) atoms. Cycle 3: After refmac, R = 0.2061 (Rfree = 0.000) for 2180 atoms. Found 31 (37 requested) and removed 22 (19 requested) atoms. Cycle 4: After refmac, R = 0.2014 (Rfree = 0.000) for 2182 atoms. Found 33 (37 requested) and removed 19 (19 requested) atoms. Cycle 5: After refmac, R = 0.1943 (Rfree = 0.000) for 2191 atoms. Found 30 (36 requested) and removed 19 (19 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 2.43 2.01 NCS extension: 4 residues added (1 deleted due to clashes), 2216 seeds are put forward Round 1: 231 peptides, 7 chains. Longest chain 93 peptides. Score 0.846 Round 2: 234 peptides, 7 chains. Longest chain 94 peptides. Score 0.850 Round 3: 232 peptides, 10 chains. Longest chain 74 peptides. Score 0.823 Round 4: 233 peptides, 7 chains. Longest chain 98 peptides. Score 0.849 Round 5: 235 peptides, 7 chains. Longest chain 100 peptides. Score 0.852 Taking the results from Round 5 Chains 7, Residues 228, Estimated correctness of the model 95.6 % 2 chains (196 residues) have been docked in sequence Building loops using Loopy2018 7 chains (228 residues) following loop building 2 chains (196 residues) in sequence following loop building ------------------------------------------------------ 14305 reflections ( 98.97 % complete ) and 2476 restraints for refining 2113 atoms. 747 conditional restraints added. Observations/parameters ratio is 1.69 ------------------------------------------------------ Cycle 6: After refmac, R = 0.2130 (Rfree = 0.000) for 2113 atoms. Found 34 (34 requested) and removed 20 (19 requested) atoms. Cycle 7: After refmac, R = 0.1964 (Rfree = 0.000) for 2117 atoms. Found 33 (33 requested) and removed 20 (19 requested) atoms. Cycle 8: After refmac, R = 0.1896 (Rfree = 0.000) for 2119 atoms. Found 23 (33 requested) and removed 19 (19 requested) atoms. Cycle 9: After refmac, R = 0.1833 (Rfree = 0.000) for 2119 atoms. Found 21 (32 requested) and removed 21 (19 requested) atoms. Cycle 10: After refmac, R = 0.1823 (Rfree = 0.000) for 2118 atoms. Found 22 (31 requested) and removed 19 (19 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 2.39 1.97 NCS extension: 54 residues added (44 deleted due to clashes), 2181 seeds are put forward Round 1: 229 peptides, 9 chains. Longest chain 68 peptides. Score 0.827 Round 2: 234 peptides, 8 chains. Longest chain 104 peptides. Score 0.842 Round 3: 233 peptides, 7 chains. Longest chain 97 peptides. Score 0.849 Round 4: 241 peptides, 5 chains. Longest chain 103 peptides. Score 0.874 Round 5: 242 peptides, 7 chains. Longest chain 110 peptides. Score 0.861 Taking the results from Round 4 Chains 5, Residues 236, Estimated correctness of the model 96.7 % 3 chains (222 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 96 A and 99 A 4 chains (238 residues) following loop building 2 chains (224 residues) in sequence following loop building ------------------------------------------------------ 14305 reflections ( 98.97 % complete ) and 2317 restraints for refining 2144 atoms. 436 conditional restraints added. Observations/parameters ratio is 1.67 ------------------------------------------------------ Cycle 11: After refmac, R = 0.2185 (Rfree = 0.000) for 2144 atoms. Found 30 (30 requested) and removed 23 (19 requested) atoms. Cycle 12: After refmac, R = 0.1946 (Rfree = 0.000) for 2143 atoms. Found 30 (30 requested) and removed 21 (19 requested) atoms. Cycle 13: After refmac, R = 0.1868 (Rfree = 0.000) for 2152 atoms. Found 21 (29 requested) and removed 19 (19 requested) atoms. Cycle 14: After refmac, R = 0.1845 (Rfree = 0.000) for 2148 atoms. Found 29 (29 requested) and removed 22 (19 requested) atoms. Cycle 15: After refmac, R = 0.1808 (Rfree = 0.000) for 2155 atoms. Found 28 (28 requested) and removed 20 (19 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 2.46 2.03 NCS extension: 79 residues added (44 deleted due to clashes), 2247 seeds are put forward Round 1: 233 peptides, 9 chains. Longest chain 65 peptides. Score 0.833 Round 2: 238 peptides, 7 chains. Longest chain 70 peptides. Score 0.856 Round 3: 241 peptides, 6 chains. Longest chain 117 peptides. Score 0.867 Round 4: 237 peptides, 8 chains. Longest chain 69 peptides. Score 0.846 Round 5: 242 peptides, 5 chains. Longest chain 92 peptides. Score 0.875 Taking the results from Round 5 Chains 5, Residues 237, Estimated correctness of the model 96.8 % 4 chains (220 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 94 A and 98 A Built loop between residues 53 B and 57 B 3 chains (243 residues) following loop building 2 chains (226 residues) in sequence following loop building ------------------------------------------------------ 14305 reflections ( 98.97 % complete ) and 2256 restraints for refining 2134 atoms. 356 conditional restraints added. Observations/parameters ratio is 1.68 ------------------------------------------------------ Cycle 16: After refmac, R = 0.2093 (Rfree = 0.000) for 2134 atoms. Found 27 (27 requested) and removed 23 (19 requested) atoms. Cycle 17: After refmac, R = 0.1919 (Rfree = 0.000) for 2133 atoms. Found 26 (26 requested) and removed 21 (19 requested) atoms. Cycle 18: After refmac, R = 0.1838 (Rfree = 0.000) for 2135 atoms. Found 25 (25 requested) and removed 20 (19 requested) atoms. Cycle 19: After refmac, R = 0.1803 (Rfree = 0.000) for 2140 atoms. Found 24 (24 requested) and removed 20 (19 requested) atoms. Cycle 20: After refmac, R = 0.1777 (Rfree = 0.000) for 2144 atoms. Found 24 (24 requested) and removed 19 (19 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 2.45 2.02 NCS extension: 0 residues added, 2155 seeds are put forward Round 1: 238 peptides, 8 chains. Longest chain 65 peptides. Score 0.848 Round 2: 238 peptides, 9 chains. Longest chain 63 peptides. Score 0.840 Round 3: 242 peptides, 6 chains. Longest chain 92 peptides. Score 0.868 Round 4: 239 peptides, 9 chains. Longest chain 87 peptides. Score 0.841 Round 5: 242 peptides, 7 chains. Longest chain 114 peptides. Score 0.861 Taking the results from Round 3 Chains 6, Residues 236, Estimated correctness of the model 96.4 % 5 chains (228 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 94 A and 98 A Built loop between residues 117 A and 120 A Built loop between residues 51 B and 55 B 3 chains (244 residues) following loop building 2 chains (236 residues) in sequence following loop building ------------------------------------------------------ 14305 reflections ( 98.97 % complete ) and 2247 restraints for refining 2158 atoms. 311 conditional restraints added. Observations/parameters ratio is 1.66 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2036 (Rfree = 0.000) for 2158 atoms. Found 23 (23 requested) and removed 21 (19 requested) atoms. Cycle 22: After refmac, R = 0.1899 (Rfree = 0.000) for 2158 atoms. Found 23 (23 requested) and removed 21 (19 requested) atoms. Cycle 23: After refmac, R = 0.1852 (Rfree = 0.000) for 2158 atoms. Found 22 (22 requested) and removed 20 (19 requested) atoms. Cycle 24: After refmac, R = 0.1795 (Rfree = 0.000) for 2160 atoms. Found 21 (21 requested) and removed 20 (19 requested) atoms. Cycle 25: After refmac, R = 0.1776 (Rfree = 0.000) for 2157 atoms. Found 20 (20 requested) and removed 20 (19 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 2.44 2.01 NCS extension: 85 residues added (49 deleted due to clashes), 2247 seeds are put forward Round 1: 240 peptides, 8 chains. Longest chain 68 peptides. Score 0.850 Round 2: 241 peptides, 8 chains. Longest chain 104 peptides. Score 0.852 Round 3: 238 peptides, 10 chains. Longest chain 88 peptides. Score 0.832 Round 4: 241 peptides, 7 chains. Longest chain 107 peptides. Score 0.859 Round 5: 235 peptides, 10 chains. Longest chain 64 peptides. Score 0.827 Taking the results from Round 4 Chains 9, Residues 234, Estimated correctness of the model 96.0 % 4 chains (201 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 94 B and 99 B 7 chains (236 residues) following loop building 3 chains (205 residues) in sequence following loop building ------------------------------------------------------ 14305 reflections ( 98.97 % complete ) and 2452 restraints for refining 2145 atoms. 655 conditional restraints added. Observations/parameters ratio is 1.67 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2059 (Rfree = 0.000) for 2145 atoms. Found 19 (19 requested) and removed 25 (19 requested) atoms. Cycle 27: After refmac, R = 0.1899 (Rfree = 0.000) for 2136 atoms. Found 19 (19 requested) and removed 20 (19 requested) atoms. Cycle 28: After refmac, R = 0.1843 (Rfree = 0.000) for 2132 atoms. Found 19 (19 requested) and removed 19 (19 requested) atoms. Cycle 29: After refmac, R = 0.1797 (Rfree = 0.000) for 2130 atoms. Found 19 (19 requested) and removed 19 (19 requested) atoms. Cycle 30: After refmac, R = 0.1794 (Rfree = 0.000) for 2127 atoms. Found 19 (19 requested) and removed 20 (19 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 2.44 2.01 NCS extension: 79 residues added (27 deleted due to clashes), 2210 seeds are put forward Round 1: 229 peptides, 8 chains. Longest chain 63 peptides. Score 0.835 Round 2: 231 peptides, 9 chains. Longest chain 75 peptides. Score 0.830 Round 3: 236 peptides, 7 chains. Longest chain 95 peptides. Score 0.853 Round 4: 235 peptides, 9 chains. Longest chain 94 peptides. Score 0.836 Round 5: 234 peptides, 10 chains. Longest chain 80 peptides. Score 0.826 Taking the results from Round 3 Chains 9, Residues 229, Estimated correctness of the model 95.7 % 6 chains (210 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 16 A and 20 A Built loop between residues 20 B and 23 B 7 chains (233 residues) following loop building 4 chains (215 residues) in sequence following loop building ------------------------------------------------------ 14305 reflections ( 98.97 % complete ) and 2427 restraints for refining 2124 atoms. 628 conditional restraints added. Observations/parameters ratio is 1.68 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2144 (Rfree = 0.000) for 2124 atoms. Found 19 (19 requested) and removed 19 (19 requested) atoms. Cycle 32: After refmac, R = 0.1996 (Rfree = 0.000) for 2124 atoms. Found 19 (19 requested) and removed 19 (19 requested) atoms. Cycle 33: After refmac, R = 0.1906 (Rfree = 0.000) for 2123 atoms. Found 19 (19 requested) and removed 21 (19 requested) atoms. Cycle 34: After refmac, R = 0.1852 (Rfree = 0.000) for 2120 atoms. Found 19 (19 requested) and removed 21 (19 requested) atoms. Cycle 35: After refmac, R = 0.1840 (Rfree = 0.000) for 2117 atoms. Found 19 (19 requested) and removed 21 (19 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 2.43 2.01 NCS extension: 125 residues added (90 deleted due to clashes), 2251 seeds are put forward Round 1: 229 peptides, 10 chains. Longest chain 65 peptides. Score 0.818 Round 2: 234 peptides, 9 chains. Longest chain 94 peptides. Score 0.834 Round 3: 237 peptides, 7 chains. Longest chain 81 peptides. Score 0.854 Round 4: 234 peptides, 8 chains. Longest chain 87 peptides. Score 0.842 Round 5: 240 peptides, 7 chains. Longest chain 101 peptides. Score 0.858 Taking the results from Round 5 Chains 9, Residues 233, Estimated correctness of the model 95.9 % 4 chains (199 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 115 A and 119 A 7 chains (235 residues) following loop building 3 chains (202 residues) in sequence following loop building ------------------------------------------------------ 14305 reflections ( 98.97 % complete ) and 2492 restraints for refining 2109 atoms. 724 conditional restraints added. Observations/parameters ratio is 1.70 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2023 (Rfree = 0.000) for 2109 atoms. Found 19 (19 requested) and removed 21 (19 requested) atoms. Cycle 37: After refmac, R = 0.1882 (Rfree = 0.000) for 2105 atoms. Found 19 (19 requested) and removed 20 (19 requested) atoms. Cycle 38: After refmac, R = 0.1853 (Rfree = 0.000) for 2103 atoms. Found 19 (19 requested) and removed 19 (19 requested) atoms. Cycle 39: After refmac, R = 0.1808 (Rfree = 0.000) for 2103 atoms. Found 19 (19 requested) and removed 17 (19 requested) atoms. Cycle 40: After refmac, R = 0.1789 (Rfree = 0.000) for 2104 atoms. Found 19 (19 requested) and removed 21 (19 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 2.44 2.01 NCS extension: 11 residues added (53 deleted due to clashes), 2120 seeds are put forward Round 1: 239 peptides, 7 chains. Longest chain 103 peptides. Score 0.857 Round 2: 239 peptides, 7 chains. Longest chain 73 peptides. Score 0.857 Round 3: 237 peptides, 7 chains. Longest chain 95 peptides. Score 0.854 Round 4: 241 peptides, 5 chains. Longest chain 101 peptides. Score 0.874 Round 5: 238 peptides, 8 chains. Longest chain 86 peptides. Score 0.848 Taking the results from Round 4 Chains 5, Residues 236, Estimated correctness of the model 96.7 % 3 chains (221 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 96 A and 99 A 4 chains (238 residues) following loop building 2 chains (223 residues) in sequence following loop building ------------------------------------------------------ 14305 reflections ( 98.97 % complete ) and 2355 restraints for refining 2141 atoms. 477 conditional restraints added. Observations/parameters ratio is 1.67 ------------------------------------------------------ Cycle 41: After refmac, R = 0.1995 (Rfree = 0.000) for 2141 atoms. Found 19 (19 requested) and removed 23 (19 requested) atoms. Cycle 42: After refmac, R = 0.1841 (Rfree = 0.000) for 2137 atoms. Found 19 (19 requested) and removed 20 (19 requested) atoms. Cycle 43: After refmac, R = 0.1819 (Rfree = 0.000) for 2134 atoms. Found 19 (19 requested) and removed 20 (19 requested) atoms. Cycle 44: After refmac, R = 0.1812 (Rfree = 0.000) for 2132 atoms. Found 19 (19 requested) and removed 21 (19 requested) atoms. Cycle 45: After refmac, R = 0.1801 (Rfree = 0.000) for 2130 atoms. Found 19 (19 requested) and removed 20 (19 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 2.43 2.01 NCS extension: 110 residues added (51 deleted due to clashes), 2246 seeds are put forward Round 1: 236 peptides, 6 chains. Longest chain 109 peptides. Score 0.861 Round 2: 236 peptides, 8 chains. Longest chain 101 peptides. Score 0.845 Round 3: 238 peptides, 7 chains. Longest chain 80 peptides. Score 0.856 Round 4: 239 peptides, 5 chains. Longest chain 110 peptides. Score 0.872 Round 5: 238 peptides, 10 chains. Longest chain 62 peptides. Score 0.832 Taking the results from Round 4 Last building cycle: Chain fragments will be rearranged Chains 5, Residues 234, Estimated correctness of the model 96.6 % 4 chains (224 residues) have been docked in sequence Sequence coverage is 95 % All DUM atoms will be removed Building loops using Loopy2018 Built loop between residues 49 A and 55 A Built loop between residues 115 A and 119 A 3 chains (242 residues) following loop building 2 chains (232 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. Update only waters. 14305 reflections ( 98.97 % complete ) and 1919 restraints for refining 1868 atoms. Observations/parameters ratio is 1.91 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2771 (Rfree = 0.000) for 1868 atoms. Found 14 (16 requested) and removed 0 (16 requested) atoms. Cycle 47: After refmac, R = 0.2500 (Rfree = 0.000) for 1868 atoms. Found 17 (17 requested) and removed 5 (17 requested) atoms. Cycle 48: After refmac, R = 0.2398 (Rfree = 0.000) for 1868 atoms. Found 12 (17 requested) and removed 6 (17 requested) atoms. Cycle 49: After refmac, R = 0.2340 (Rfree = 0.000) for 1868 atoms. Found 12 (17 requested) and removed 5 (17 requested) atoms. Writing output files ... Normal termination of warpNtrace Sun 23 Dec 23:15:29 GMT 2018 Job finished. TimeTaking 52.5 Used memory is bytes: 9489880