null Sun 23 Dec 22:22:44 GMT 2018 ############### You are running ARP/wARP 8.0 ############### Working directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir Job ID is set to 1ztc-3.2-parrot-hancs X-ray data file /users/emra500/scratch/Dataset/hancsChltofom/1ztc-3.2-parrot-hancs.mtz Sequence file /users/emra500/scratch/Dataset/hancsChltofom/1ztc-3.2-parrot-hancs.fasta Tracing mode WARPNTRACEPHASES Creating directory /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/1ztc-3.2-parrot-hancs Accepted label: fp=FP Accepted label: sigfp=SIGFP Accepted label: phibest=hltofom.Phi_fom.phi Accepted label: fom=hltofom.Phi_fom.fom mtz labels taken: FP SIGFP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Parameter file /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/1ztc-3.2-parrot-hancs/arp_warp_classic.par Job launched in /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/1ztc-3.2-parrot-hancs ############### You are running ARP/wARP 8.0 ############### ============================================================ Please cite at least one of the publications below for the ARP/wARP application you are using: Lamzin VS, Wilson KS. (1993) Automated refinement of protein models. Acta Crystallogr D Biol Crystallogr. 49, 129-147 Morris RJ, Perrakis A. Lamzin VS. (2003) ARP/wARP and automatic interpretation of protein electron density maps. In Methods Enzymol. (Carter, C.W. & Sweet, R.M. eds.) 374, 229-244 Langer GG, Hazledine S, Wiegels T, Carolan C, Lamzin VS. (2013) Visual automated macromolecular model building. Acta Crystallogr D Biol Crystallogr. 69, 635-641 Use of Refmac is an essential part of model building with ARP/wARP. Please acknowledge the use of Refmac by citing the following publication: Murshudov GN, Skubak P, Lebedev AA, Pannu NS, Steiner RA, Nicholls RA, Winn MD, Long F, Vagin AA. (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 67, 355-367 ============================================================ Sun 23 Dec 22:22:52 GMT 2018 You are running ARP/wARP version 8.0 Entering warp_tracing.sh from COMMAND_LINE_SUBMISSION The working directory: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/1ztc-3.2-parrot-hancs ARP/wARP software version: 8.0 The parameter file: /mnt/lustre/users/emra500/PipeliensFastaNoRFree/hancs/ArpWArp/wArpResults/WorkingDir/1ztc-3.2-parrot-hancs/arp_warp_classic.par ARP/wARP will run in subdirectory: temp_tracing Checking the estimated solvent content Computed solvent content is outside allowed range. Target number of residues/nucleotides is changed to 1182 and 0 Target number of residues in the AU: 1182 Target solvent content: 0.6324 Checking the provided sequence file Detected sequence length: 221 Maximum limit of NCS related copies: 60 Number of NCS-related molecules: 4 Adjusted target number of residues: 884 Adjusted target solvent content: 0.73 Input MTZ file: 1ztc-3.2-parrot-hancs.mtz.cad Column labels for model refinement: FP SIGFP Column labels to obtain initial map: FP hltofom.Phi_fom.phi hltofom.Phi_fom.fom Space group number: 19 Cell parameters: 89.783 127.343 152.224 90.000 90.000 90.000 Input sequence file: 1ztc-3.2-parrot-hancs.fasta_lf Building free atoms model in initial map for 7072 target number of atoms Had to go as low as 1.10 sigma, to complete atoms search. ARP/wARP will be iterated with REFMAC5 50 refinement / model update cycles will be run in total. Atoms will be removed below 1.0 sigma in 2mFoDFc map and added above 3.2 sigma in mFoDFc map. Refmac Refinement Parameters 3 REFMAC cycle(s) with 1.0 1.0 fractional shifts damping in each cycle. Conditional restraints will be used Weight for restraints is set to AUTO Solvent mask correction will be used Anisotropic scaling with no bulk solvent correction Model Building Parameters Main chain tracing will take place every 5 refinement / model update cycles. Side chain docking will be performed from autobuilding cycle number 1 NCS extension will be performed at every building cycle. NCS restraints will be applied at each refinement cycle. Loops will be built when appropriate and at the last cycle Resolution range: 97.673 3.200 Wilson plot Bfac: 67.79 29180 reflections ( 98.97 % complete ) and 0 restraints for refining 7811 atoms. Observations/parameters ratio is 0.93 ------------------------------------------------------ Starting model: R = 0.3178 (Rfree = 0.000). ------------------------------------------------------ Cycle 0: After refmac, R = 0.2893 (Rfree = 0.000) for 7811 atoms. Found 70 (70 requested) and removed 41 (35 requested) atoms. ------------------------------------------------------ Building Cycle 1 Atomic shape factors 3.20 2.30 Search for helices and strands: 0 residues in 0 chains, 7997 seeds are put forward NCS extension: 0 residues added, 7997 seeds are put forward Round 1: 634 peptides, 72 chains. Longest chain 28 peptides. Score 0.526 Round 2: 691 peptides, 64 chains. Longest chain 31 peptides. Score 0.599 Round 3: 709 peptides, 54 chains. Longest chain 37 peptides. Score 0.645 Round 4: 683 peptides, 60 chains. Longest chain 33 peptides. Score 0.607 Round 5: 712 peptides, 55 chains. Longest chain 44 peptides. Score 0.644 Taking the results from Round 3 Chains 60, Residues 655, Estimated correctness of the model 52.6 % 8 chains (158 residues) have been docked in sequence ------------------------------------------------------ 29180 reflections ( 98.97 % complete ) and 12638 restraints for refining 6874 atoms. 9324 conditional restraints added. Observations/parameters ratio is 1.06 ------------------------------------------------------ Cycle 1: After refmac, R = 0.2681 (Rfree = 0.000) for 6874 atoms. Found 61 (61 requested) and removed 59 (30 requested) atoms. Cycle 2: After refmac, R = 0.2510 (Rfree = 0.000) for 6748 atoms. Found 61 (61 requested) and removed 39 (30 requested) atoms. Cycle 3: After refmac, R = 0.2418 (Rfree = 0.000) for 6730 atoms. Found 56 (60 requested) and removed 52 (30 requested) atoms. Cycle 4: After refmac, R = 0.2354 (Rfree = 0.000) for 6715 atoms. Found 38 (60 requested) and removed 33 (30 requested) atoms. Cycle 5: After refmac, R = 0.2330 (Rfree = 0.000) for 6705 atoms. Found 41 (60 requested) and removed 34 (30 requested) atoms. ------------------------------------------------------ Building Cycle 2 Atomic shape factors 3.22 2.31 Search for helices and strands: 0 residues in 0 chains, 6899 seeds are put forward NCS extension: 11 residues added (9 deleted due to clashes), 6910 seeds are put forward Round 1: 693 peptides, 58 chains. Longest chain 34 peptides. Score 0.621 Round 2: 728 peptides, 54 chains. Longest chain 48 peptides. Score 0.657 Round 3: 708 peptides, 57 chains. Longest chain 47 peptides. Score 0.634 Round 4: 713 peptides, 57 chains. Longest chain 45 peptides. Score 0.638 Round 5: 715 peptides, 47 chains. Longest chain 53 peptides. Score 0.671 Taking the results from Round 5 Chains 55, Residues 668, Estimated correctness of the model 58.8 % 11 chains (290 residues) have been docked in sequence ------------------------------------------------------ 29180 reflections ( 98.97 % complete ) and 10749 restraints for refining 6617 atoms. 6856 conditional restraints added. Observations/parameters ratio is 1.10 ------------------------------------------------------ Cycle 6: After refmac, R = 0.2567 (Rfree = 0.000) for 6617 atoms. Found 59 (59 requested) and removed 64 (29 requested) atoms. Cycle 7: After refmac, R = 0.2524 (Rfree = 0.000) for 6553 atoms. Found 59 (59 requested) and removed 41 (29 requested) atoms. Cycle 8: After refmac, R = 0.2258 (Rfree = 0.000) for 6526 atoms. Found 45 (58 requested) and removed 33 (29 requested) atoms. Cycle 9: After refmac, R = 0.2138 (Rfree = 0.000) for 6497 atoms. Found 36 (58 requested) and removed 34 (29 requested) atoms. Cycle 10: After refmac, R = 0.2074 (Rfree = 0.000) for 6483 atoms. Found 28 (58 requested) and removed 35 (29 requested) atoms. ------------------------------------------------------ Building Cycle 3 Atomic shape factors 3.20 2.30 Search for helices and strands: 0 residues in 0 chains, 6670 seeds are put forward NCS extension: 22 residues added (26 deleted due to clashes), 6692 seeds are put forward Round 1: 707 peptides, 54 chains. Longest chain 33 peptides. Score 0.644 Round 2: 743 peptides, 52 chains. Longest chain 50 peptides. Score 0.673 Round 3: 730 peptides, 51 chains. Longest chain 51 peptides. Score 0.668 Round 4: 734 peptides, 44 chains. Longest chain 59 peptides. Score 0.692 Round 5: 730 peptides, 48 chains. Longest chain 65 peptides. Score 0.677 Taking the results from Round 4 Chains 50, Residues 690, Estimated correctness of the model 63.5 % 12 chains (353 residues) have been docked in sequence ------------------------------------------------------ 29180 reflections ( 98.97 % complete ) and 10986 restraints for refining 6861 atoms. 6716 conditional restraints added. Observations/parameters ratio is 1.06 ------------------------------------------------------ Cycle 11: After refmac, R = 0.2551 (Rfree = 0.000) for 6861 atoms. Found 61 (61 requested) and removed 70 (30 requested) atoms. Cycle 12: After refmac, R = 0.2506 (Rfree = 0.000) for 6801 atoms. Found 61 (61 requested) and removed 45 (30 requested) atoms. Cycle 13: After refmac, R = 0.2454 (Rfree = 0.000) for 6772 atoms. Found 61 (61 requested) and removed 46 (30 requested) atoms. Cycle 14: After refmac, R = 0.2414 (Rfree = 0.000) for 6754 atoms. Found 60 (60 requested) and removed 45 (30 requested) atoms. Cycle 15: After refmac, R = 0.2376 (Rfree = 0.000) for 6737 atoms. Found 60 (60 requested) and removed 46 (30 requested) atoms. ------------------------------------------------------ Building Cycle 4 Atomic shape factors 3.19 2.29 Search for helices and strands: 0 residues in 0 chains, 6947 seeds are put forward NCS extension: 12 residues added (51 deleted due to clashes), 6959 seeds are put forward Round 1: 720 peptides, 56 chains. Longest chain 57 peptides. Score 0.645 Round 2: 731 peptides, 49 chains. Longest chain 63 peptides. Score 0.675 Round 3: 736 peptides, 51 chains. Longest chain 73 peptides. Score 0.671 Round 4: 737 peptides, 47 chains. Longest chain 44 peptides. Score 0.684 Round 5: 749 peptides, 44 chains. Longest chain 51 peptides. Score 0.700 Taking the results from Round 5 Chains 49, Residues 705, Estimated correctness of the model 65.2 % 14 chains (417 residues) have been docked in sequence ------------------------------------------------------ 29180 reflections ( 98.97 % complete ) and 10209 restraints for refining 6787 atoms. 5609 conditional restraints added. Observations/parameters ratio is 1.07 ------------------------------------------------------ Cycle 16: After refmac, R = 0.2649 (Rfree = 0.000) for 6787 atoms. Found 60 (60 requested) and removed 84 (30 requested) atoms. Cycle 17: After refmac, R = 0.2485 (Rfree = 0.000) for 6729 atoms. Found 60 (60 requested) and removed 51 (30 requested) atoms. Cycle 18: After refmac, R = 0.2448 (Rfree = 0.000) for 6711 atoms. Found 60 (60 requested) and removed 54 (30 requested) atoms. Cycle 19: After refmac, R = 0.2329 (Rfree = 0.000) for 6698 atoms. Found 60 (60 requested) and removed 43 (30 requested) atoms. Cycle 20: After refmac, R = 0.2319 (Rfree = 0.000) for 6698 atoms. Found 60 (60 requested) and removed 54 (30 requested) atoms. ------------------------------------------------------ Building Cycle 5 Atomic shape factors 3.21 2.30 Search for helices and strands: 0 residues in 0 chains, 6861 seeds are put forward NCS extension: 14 residues added (38 deleted due to clashes), 6875 seeds are put forward Round 1: 744 peptides, 51 chains. Longest chain 50 peptides. Score 0.676 Round 2: 758 peptides, 48 chains. Longest chain 51 peptides. Score 0.694 Round 3: 743 peptides, 40 chains. Longest chain 53 peptides. Score 0.709 Round 4: 721 peptides, 52 chains. Longest chain 38 peptides. Score 0.659 Round 5: 742 peptides, 50 chains. Longest chain 55 peptides. Score 0.678 Taking the results from Round 3 Chains 51, Residues 703, Estimated correctness of the model 67.1 % 17 chains (423 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 140 C and 144 C Built loop between residues 195 C and 199 C Built loop between residues 148 D and 155 D 46 chains (710 residues) following loop building 14 chains (435 residues) in sequence following loop building ------------------------------------------------------ 29180 reflections ( 98.97 % complete ) and 10054 restraints for refining 6743 atoms. 5373 conditional restraints added. Observations/parameters ratio is 1.08 ------------------------------------------------------ Cycle 21: After refmac, R = 0.2406 (Rfree = 0.000) for 6743 atoms. Found 60 (60 requested) and removed 60 (30 requested) atoms. Cycle 22: After refmac, R = 0.2217 (Rfree = 0.000) for 6703 atoms. Found 55 (59 requested) and removed 41 (30 requested) atoms. Cycle 23: After refmac, R = 0.2146 (Rfree = 0.000) for 6697 atoms. Found 38 (58 requested) and removed 35 (30 requested) atoms. Cycle 24: After refmac, R = 0.2099 (Rfree = 0.000) for 6682 atoms. Found 51 (56 requested) and removed 36 (30 requested) atoms. Cycle 25: After refmac, R = 0.2166 (Rfree = 0.000) for 6686 atoms. Found 55 (55 requested) and removed 37 (30 requested) atoms. ------------------------------------------------------ Building Cycle 6 Atomic shape factors 3.21 2.30 Search for helices and strands: 0 residues in 0 chains, 6849 seeds are put forward NCS extension: 14 residues added (55 deleted due to clashes), 6863 seeds are put forward Round 1: 719 peptides, 55 chains. Longest chain 50 peptides. Score 0.648 Round 2: 731 peptides, 54 chains. Longest chain 49 peptides. Score 0.659 Round 3: 738 peptides, 53 chains. Longest chain 52 peptides. Score 0.666 Round 4: 737 peptides, 41 chains. Longest chain 51 peptides. Score 0.702 Round 5: 717 peptides, 44 chains. Longest chain 51 peptides. Score 0.682 Taking the results from Round 4 Chains 53, Residues 696, Estimated correctness of the model 65.6 % 17 chains (453 residues) have been docked in sequence Building loops using Loopy2018 Built loop between residues 168 A and 177 A Built loop between residues 192 A and 196 A Built loop between residues 26 B and 29 B 48 chains (706 residues) following loop building 14 chains (466 residues) in sequence following loop building ------------------------------------------------------ 29180 reflections ( 98.97 % complete ) and 10092 restraints for refining 6893 atoms. 5256 conditional restraints added. Observations/parameters ratio is 1.06 ------------------------------------------------------ Cycle 26: After refmac, R = 0.2562 (Rfree = 0.000) for 6893 atoms. Found 55 (55 requested) and removed 65 (30 requested) atoms. Cycle 27: After refmac, R = 0.2437 (Rfree = 0.000) for 6843 atoms. Found 54 (54 requested) and removed 49 (30 requested) atoms. Cycle 28: After refmac, R = 0.2421 (Rfree = 0.000) for 6824 atoms. Found 52 (52 requested) and removed 44 (30 requested) atoms. Cycle 29: After refmac, R = 0.2381 (Rfree = 0.000) for 6807 atoms. Found 51 (51 requested) and removed 44 (30 requested) atoms. Cycle 30: After refmac, R = 0.2337 (Rfree = 0.000) for 6794 atoms. Found 50 (50 requested) and removed 37 (30 requested) atoms. ------------------------------------------------------ Building Cycle 7 Atomic shape factors 3.21 2.30 Search for helices and strands: 0 residues in 0 chains, 6983 seeds are put forward NCS extension: 15 residues added (47 deleted due to clashes), 6998 seeds are put forward Round 1: 718 peptides, 49 chains. Longest chain 39 peptides. Score 0.667 Round 2: 744 peptides, 46 chains. Longest chain 79 peptides. Score 0.692 Round 3: 733 peptides, 50 chains. Longest chain 59 peptides. Score 0.673 Round 4: 738 peptides, 53 chains. Longest chain 38 peptides. Score 0.666 Round 5: 740 peptides, 47 chains. Longest chain 54 peptides. Score 0.686 Taking the results from Round 2 Chains 55, Residues 698, Estimated correctness of the model 63.5 % 14 chains (375 residues) have been docked in sequence ------------------------------------------------------ 29180 reflections ( 98.97 % complete ) and 10537 restraints for refining 6693 atoms. 6163 conditional restraints added. Observations/parameters ratio is 1.09 ------------------------------------------------------ Cycle 31: After refmac, R = 0.2578 (Rfree = 0.000) for 6693 atoms. Found 48 (48 requested) and removed 83 (30 requested) atoms. Cycle 32: After refmac, R = 0.2452 (Rfree = 0.000) for 6627 atoms. Found 47 (47 requested) and removed 43 (29 requested) atoms. Cycle 33: After refmac, R = 0.2336 (Rfree = 0.000) for 6608 atoms. Found 47 (47 requested) and removed 55 (29 requested) atoms. Cycle 34: After refmac, R = 0.2325 (Rfree = 0.000) for 6578 atoms. Found 47 (47 requested) and removed 56 (29 requested) atoms. Cycle 35: After refmac, R = 0.2285 (Rfree = 0.000) for 6553 atoms. Found 46 (46 requested) and removed 49 (29 requested) atoms. ------------------------------------------------------ Building Cycle 8 Atomic shape factors 3.17 2.28 Search for helices and strands: 0 residues in 0 chains, 6673 seeds are put forward NCS extension: 9 residues added (24 deleted due to clashes), 6682 seeds are put forward Round 1: 712 peptides, 49 chains. Longest chain 51 peptides. Score 0.663 Round 2: 735 peptides, 45 chains. Longest chain 51 peptides. Score 0.689 Round 3: 745 peptides, 48 chains. Longest chain 54 peptides. Score 0.686 Round 4: 741 peptides, 44 chains. Longest chain 50 peptides. Score 0.696 Round 5: 742 peptides, 50 chains. Longest chain 41 peptides. Score 0.678 Taking the results from Round 4 Chains 52, Residues 697, Estimated correctness of the model 64.3 % 13 chains (347 residues) have been docked in sequence ------------------------------------------------------ 29180 reflections ( 98.97 % complete ) and 9958 restraints for refining 6467 atoms. 5679 conditional restraints added. Observations/parameters ratio is 1.13 ------------------------------------------------------ Cycle 36: After refmac, R = 0.2561 (Rfree = 0.000) for 6467 atoms. Found 46 (46 requested) and removed 68 (29 requested) atoms. Cycle 37: After refmac, R = 0.2464 (Rfree = 0.000) for 6403 atoms. Found 46 (46 requested) and removed 48 (28 requested) atoms. Cycle 38: After refmac, R = 0.2408 (Rfree = 0.000) for 6389 atoms. Found 46 (46 requested) and removed 47 (28 requested) atoms. Cycle 39: After refmac, R = 0.2346 (Rfree = 0.000) for 6367 atoms. Found 46 (46 requested) and removed 40 (28 requested) atoms. Cycle 40: After refmac, R = 0.2303 (Rfree = 0.000) for 6363 atoms. Found 46 (46 requested) and removed 35 (28 requested) atoms. ------------------------------------------------------ Building Cycle 9 Atomic shape factors 3.21 2.30 Search for helices and strands: 0 residues in 0 chains, 6584 seeds are put forward NCS extension: 12 residues added (39 deleted due to clashes), 6596 seeds are put forward Round 1: 711 peptides, 58 chains. Longest chain 52 peptides. Score 0.633 Round 2: 731 peptides, 47 chains. Longest chain 54 peptides. Score 0.681 Round 3: 729 peptides, 45 chains. Longest chain 51 peptides. Score 0.686 Round 4: 738 peptides, 43 chains. Longest chain 54 peptides. Score 0.697 Round 5: 729 peptides, 47 chains. Longest chain 42 peptides. Score 0.680 Taking the results from Round 4 Chains 52, Residues 695, Estimated correctness of the model 64.5 % 16 chains (428 residues) have been docked in sequence ------------------------------------------------------ 29180 reflections ( 98.97 % complete ) and 9914 restraints for refining 6689 atoms. 5264 conditional restraints added. Observations/parameters ratio is 1.09 ------------------------------------------------------ Cycle 41: After refmac, R = 0.2557 (Rfree = 0.000) for 6689 atoms. Found 48 (48 requested) and removed 51 (30 requested) atoms. Cycle 42: After refmac, R = 0.2434 (Rfree = 0.000) for 6649 atoms. Found 48 (48 requested) and removed 42 (30 requested) atoms. Cycle 43: After refmac, R = 0.2363 (Rfree = 0.000) for 6628 atoms. Found 47 (47 requested) and removed 36 (29 requested) atoms. Cycle 44: After refmac, R = 0.2332 (Rfree = 0.000) for 6622 atoms. Found 47 (47 requested) and removed 43 (29 requested) atoms. Cycle 45: After refmac, R = 0.2332 (Rfree = 0.000) for 6611 atoms. Found 47 (47 requested) and removed 39 (29 requested) atoms. ------------------------------------------------------ Building Cycle 10 Atomic shape factors 3.20 2.30 Search for helices and strands: 0 residues in 0 chains, 6814 seeds are put forward NCS extension: 9 residues added (18 deleted due to clashes), 6823 seeds are put forward Round 1: 678 peptides, 61 chains. Longest chain 37 peptides. Score 0.600 Round 2: 731 peptides, 55 chains. Longest chain 40 peptides. Score 0.656 Round 3: 736 peptides, 45 chains. Longest chain 65 peptides. Score 0.690 Round 4: 720 peptides, 49 chains. Longest chain 58 peptides. Score 0.668 Round 5: 710 peptides, 45 chains. Longest chain 54 peptides. Score 0.674 Taking the results from Round 3 Last building cycle: Chain fragments will be rearranged Chains 54, Residues 691, Estimated correctness of the model 63.0 % 15 chains (440 residues) have been docked in sequence Sequence coverage is 63 % Consider running further cycles of model building using 1ztc-3_warpNtrace.pdb as input Building loops using Loopy2018 Built loop between residues 41 C and 45 C Built loop between residues 107 C and 113 C Built loop between residues 182 C and 191 C Built loop between residues 102 D and 107 D 47 chains (702 residues) following loop building 11 chains (460 residues) in sequence following loop building ------------------------------------------------------ ---> Final restrained refinement block. No atom update. 29180 reflections ( 98.97 % complete ) and 9988 restraints for refining 6790 atoms. 5218 conditional restraints added. Observations/parameters ratio is 1.07 ------------------------------------------------------ Cycle 46: After refmac, R = 0.2449 (Rfree = 0.000) for 6790 atoms. Found 0 (48 requested) and removed 30 (30 requested) atoms. Cycle 47: After refmac, R = 0.2375 (Rfree = 0.000) for 6734 atoms. Found 0 (48 requested) and removed 24 (30 requested) atoms. Cycle 48: After refmac, R = 0.2334 (Rfree = 0.000) for 6687 atoms. Found 0 (48 requested) and removed 29 (30 requested) atoms. Cycle 49: After refmac, R = 0.2289 (Rfree = 0.000) for 6651 atoms. Found 0 (47 requested) and removed 24 (29 requested) atoms. Writing output files ... Normal termination of warpNtrace Mon 24 Dec 00:08:56 GMT 2018 Job finished. TimeTaking 106.22 Used memory is bytes: 6020464